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Comparison of human myofibrillar protein catabolic rate derived from 3-methylhistidine excretion with synthetic rate from muscle biopsies during L-(. cap alpha. -/sup 15/N)lysine infusion

Journal Article:

Abstract

Urine was collected in five healthy men over 10 to 14 days, with fasting blood samples on days 1, 5 and 10, whilst they consumed a standard creatine-free diet, which was quantitatively related to their body surface area. The urinary excretion of 3-methylhistidine fell to a plateau by day 5 in all subjects. Myofibrillar protein catabolic rate calculated from the mean value of 3-methylhistidine excretion from day 5 to day 10 averaged 1.21 g day/sup -1/ kg/sup -1/ body weight. The average turnover of muscle myofibrillar protein was calculated to be 2.16%/day. From a previous study using continuous intravenous infusion of L-(a-/sup 15/N)lysine with serial muscle biopsies on the same subjects, the mean myofibrillar protein synthetic rate was calculated to be 0.82 g day/sup -1/ kg/sup -1/ body weight, and the mean turnover rate was 1.47%/day of total muscle myofibrillar protein. The estimations of myofibrillar protein turnover rate derived from the two methods are compared and the differences discussed.
Authors:
McKeran, R O; Halliday, D; Purkiss, P [1] 
  1. Clinical Research Centre, Harrow (UK). Div. of Inherited Metabolic Diseases and Clinical Investigation
Publication Date:
May 01, 1978
Product Type:
Journal Article
Reference Number:
AIX-10-426355; EDB-79-107168
Resource Relation:
Journal Name: Clin. Sci. Mol. Med.; (United Kingdom); Journal Volume: 54:5
Subject:
59 BASIC BIOLOGICAL SCIENCES; MUSCLES; BIOCHEMICAL REACTION KINETICS; PROTEINS; METABOLISM; AMINO ACIDS; ANIMALS; AZOLES; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; BODY FLUIDS; CARBOXYLIC ACIDS; CHEMICAL REACTIONS; CLEARANCE; DECOMPOSITION; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; IMIDAZOLES; KINETICS; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PRIMATES; REACTION KINETICS; VERTEBRATES; WASTES; 550501* - Metabolism- Tracer Techniques
OSTI ID:
6253673
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: CSMMC
Submitting Site:
INIS
Size:
Pages: 471-475
Announcement Date:
Apr 01, 1979

Journal Article:

Citation Formats

McKeran, R O, Halliday, D, and Purkiss, P. Comparison of human myofibrillar protein catabolic rate derived from 3-methylhistidine excretion with synthetic rate from muscle biopsies during L-(. cap alpha. -/sup 15/N)lysine infusion. United Kingdom: N. p., 1978. Web.
McKeran, R O, Halliday, D, & Purkiss, P. Comparison of human myofibrillar protein catabolic rate derived from 3-methylhistidine excretion with synthetic rate from muscle biopsies during L-(. cap alpha. -/sup 15/N)lysine infusion. United Kingdom.
McKeran, R O, Halliday, D, and Purkiss, P. 1978. "Comparison of human myofibrillar protein catabolic rate derived from 3-methylhistidine excretion with synthetic rate from muscle biopsies during L-(. cap alpha. -/sup 15/N)lysine infusion." United Kingdom.
@misc{etde_6253673,
title = {Comparison of human myofibrillar protein catabolic rate derived from 3-methylhistidine excretion with synthetic rate from muscle biopsies during L-(. cap alpha. -/sup 15/N)lysine infusion}
author = {McKeran, R O, Halliday, D, and Purkiss, P}
abstractNote = {Urine was collected in five healthy men over 10 to 14 days, with fasting blood samples on days 1, 5 and 10, whilst they consumed a standard creatine-free diet, which was quantitatively related to their body surface area. The urinary excretion of 3-methylhistidine fell to a plateau by day 5 in all subjects. Myofibrillar protein catabolic rate calculated from the mean value of 3-methylhistidine excretion from day 5 to day 10 averaged 1.21 g day/sup -1/ kg/sup -1/ body weight. The average turnover of muscle myofibrillar protein was calculated to be 2.16%/day. From a previous study using continuous intravenous infusion of L-(a-/sup 15/N)lysine with serial muscle biopsies on the same subjects, the mean myofibrillar protein synthetic rate was calculated to be 0.82 g day/sup -1/ kg/sup -1/ body weight, and the mean turnover rate was 1.47%/day of total muscle myofibrillar protein. The estimations of myofibrillar protein turnover rate derived from the two methods are compared and the differences discussed.}
journal = {Clin. Sci. Mol. Med.; (United Kingdom)}
volume = {54:5}
journal type = {AC}
place = {United Kingdom}
year = {1978}
month = {May}
}