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Interference of aldehyde metabolizing enzyme with diamine oxidase/histaminase/activity as determined by /sup 14/C putrescine method

Abstract

The ..delta../sup 1/ pyrroline formation, as an indicator of diamine oxidase activity according to Okuyama and Kobayashi /sup 14/C putrescine test (1961, Archs Biochem. Biophys., vol.95, 242), has been investigated in several tissue homogenates. When guinea pig liver homogenate was used as a source of enzyme in the presence of aldehyde dehydrogenase inhibitors chlorate hydrate and acetaldehyde the level of formation ..delta../sup 1/ pyrroline was strongly increased in a dose-dependent manner. Also inhibition of aldehyde reductase by phenobarbital enhanced ..delta../sup 1/ pyrroline formation, but to a lesser degree. In other tissues, with very high initial diamine oxidase activity (rat intestine, dog kidney) or with very low diamine oxidase activity (guinea pig skin, dog liver) the influence of these inhibitors was only slight. Pyrazole, an inhibitor of alcohol dehydrogenase exerted only a small effect on ..delta../sup 1/ pyrroline formation. All aldehyde-metabolizing enzymes inhibitors, except pyrazole, were without effect on purified pea seddling and hog kidney diamine oxidases. The use of aldehyde-metabolizing enzymes inhibitors may help to reveal the real values of diamine oxidase activity, when tissues homogenates are used as a source of enzyme.
Authors:
Fogel, W A; [1]  Bieganski, T; Wozniak, J; Maslinski, C
  1. Polish Academy of Sciences, Cracow (Poland). Inst. of Pharmacology
Publication Date:
Jan 01, 1978
Product Type:
Journal Article
Reference Number:
AIX-10-426393; EDB-79-107177
Resource Relation:
Journal Name: Biochem. Pharmacol.; (United Kingdom); Journal Volume: 27:8
Subject:
59 BASIC BIOLOGICAL SCIENCES; HISTAMINASE; BIOCHEMICAL REACTION KINETICS; OXIDASES; CARBON 14 COMPOUNDS; CATALYSIS; COMPARATIVE EVALUATIONS; DEHYDROGENASES; ERRORS; INHIBITION; INTESTINES; KIDNEYS; LIVER; METABOLISM; PUTRESCINE; PYRROLES; QUANTITY RATIO; RESPONSE MODIFYING FACTORS; SKIN; SPECIFICITY; SUBSTRATES; TISSUES; TRACER TECHNIQUES; AMINES; AZOLES; BODY; DIGESTIVE SYSTEM; ENZYMES; GASTROINTESTINAL TRACT; GLANDS; HETEROCYCLIC COMPOUNDS; ISOTOPE APPLICATIONS; KINETICS; LABELLED COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; OXIDOREDUCTASES; REACTION KINETICS; 550501* - Metabolism- Tracer Techniques
OSTI ID:
6253564
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BCPCA
Submitting Site:
INIS
Size:
Pages: 1159-1162
Announcement Date:

Citation Formats

Fogel, W A, Bieganski, T, Wozniak, J, and Maslinski, C. Interference of aldehyde metabolizing enzyme with diamine oxidase/histaminase/activity as determined by /sup 14/C putrescine method. United Kingdom: N. p., 1978. Web. doi:10.1016/0006-2952(78)90445-8.
Fogel, W A, Bieganski, T, Wozniak, J, & Maslinski, C. Interference of aldehyde metabolizing enzyme with diamine oxidase/histaminase/activity as determined by /sup 14/C putrescine method. United Kingdom. doi:10.1016/0006-2952(78)90445-8.
Fogel, W A, Bieganski, T, Wozniak, J, and Maslinski, C. 1978. "Interference of aldehyde metabolizing enzyme with diamine oxidase/histaminase/activity as determined by /sup 14/C putrescine method." United Kingdom. doi:10.1016/0006-2952(78)90445-8. https://www.osti.gov/servlets/purl/10.1016/0006-2952(78)90445-8.
@misc{etde_6253564,
title = {Interference of aldehyde metabolizing enzyme with diamine oxidase/histaminase/activity as determined by /sup 14/C putrescine method}
author = {Fogel, W A, Bieganski, T, Wozniak, J, and Maslinski, C}
abstractNote = {The ..delta../sup 1/ pyrroline formation, as an indicator of diamine oxidase activity according to Okuyama and Kobayashi /sup 14/C putrescine test (1961, Archs Biochem. Biophys., vol.95, 242), has been investigated in several tissue homogenates. When guinea pig liver homogenate was used as a source of enzyme in the presence of aldehyde dehydrogenase inhibitors chlorate hydrate and acetaldehyde the level of formation ..delta../sup 1/ pyrroline was strongly increased in a dose-dependent manner. Also inhibition of aldehyde reductase by phenobarbital enhanced ..delta../sup 1/ pyrroline formation, but to a lesser degree. In other tissues, with very high initial diamine oxidase activity (rat intestine, dog kidney) or with very low diamine oxidase activity (guinea pig skin, dog liver) the influence of these inhibitors was only slight. Pyrazole, an inhibitor of alcohol dehydrogenase exerted only a small effect on ..delta../sup 1/ pyrroline formation. All aldehyde-metabolizing enzymes inhibitors, except pyrazole, were without effect on purified pea seddling and hog kidney diamine oxidases. The use of aldehyde-metabolizing enzymes inhibitors may help to reveal the real values of diamine oxidase activity, when tissues homogenates are used as a source of enzyme.}
doi = {10.1016/0006-2952(78)90445-8}
journal = {Biochem. Pharmacol.; (United Kingdom)}
volume = {27:8}
journal type = {AC}
place = {United Kingdom}
year = {1978}
month = {Jan}
}