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Solid-phase radioimmunoassay of immunoglobulins G, A and M: applicability in analysis of sucrose gradients

Journal Article:

Abstract

A simple and sensitive solid-phase radioimmunoassay for the detection of immunoglobulins G, A and M in sucrose gradients is described. The solid-phase consisted of immunoglobulins adsorbed to polystyrene tubes. Using buffers without detergent and /sup 125/I-labeled sheep anti-rabbit IgA as radioligand, the assay was able to detect 0.8 ng per tube in the IgG assay and 1.6 ng per tube in the IgA and IgM assays. Standard curves with antigen dissolved in 10% and 32% sucrose were superimposable and did not deviate from standard curves with antigen dissolved in buffer without sucrose. Using these techniques on ultracentrifugation samples from patients with systemic lupus erythematosus, Schoenlein-Henoch nephritis and IgA glorulonephritis is was possible to detect both immunoglobulin fragments and immunoglobulin aggregates at the same time without prior dialysis of the samples.
Authors:
Eriksen, E F; Danielsen, H; [1]  Johansen, A S; [2]  Larsson, L I [3] 
  1. Aarhus Kommunehospital (Denmark). Medical Department C
  2. Aarhus Univ. (Denmark). Institute of Medical Biochemistry
  3. Unit of Histochemistry, University Institute of Pathology, Copenhagen, Denmark
Publication Date:
Jan 01, 1984
Product Type:
Journal Article
Reference Number:
AIX-15-070066; EDB-85-029693
Resource Relation:
Journal Name: J. Immunol. Methods; (Netherlands); Journal Volume: 71:2
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; IMMUNOGLOBULINS; RADIOIMMUNOASSAY; IODINE COMPOUNDS; LABELLED COMPOUNDS; POLYSTYRENE; SACCHAROSE; SOLID CLUSTERS; ULTRACENTRIFUGATION; CARBOHYDRATES; CENTRIFUGATION; DISACCHARIDES; GLOBULINS; HALOGEN COMPOUNDS; ISOTOPE APPLICATIONS; MATERIALS; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; ORGANIC POLYMERS; PETROCHEMICALS; PETROLEUM PRODUCTS; PLASTICS; POLYMERS; POLYOLEFINS; POLYVINYLS; PROTEINS; RADIOASSAY; SACCHARIDES; SEPARATION PROCESSES; SYNTHETIC MATERIALS; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
6239772
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JIMMB
Submitting Site:
HEDB
Size:
Pages: 127-132
Announcement Date:

Journal Article:

Citation Formats

Eriksen, E F, Danielsen, H, Johansen, A S, and Larsson, L I. Solid-phase radioimmunoassay of immunoglobulins G, A and M: applicability in analysis of sucrose gradients. Netherlands: N. p., 1984. Web.
Eriksen, E F, Danielsen, H, Johansen, A S, & Larsson, L I. Solid-phase radioimmunoassay of immunoglobulins G, A and M: applicability in analysis of sucrose gradients. Netherlands.
Eriksen, E F, Danielsen, H, Johansen, A S, and Larsson, L I. 1984. "Solid-phase radioimmunoassay of immunoglobulins G, A and M: applicability in analysis of sucrose gradients." Netherlands.
@misc{etde_6239772,
title = {Solid-phase radioimmunoassay of immunoglobulins G, A and M: applicability in analysis of sucrose gradients}
author = {Eriksen, E F, Danielsen, H, Johansen, A S, and Larsson, L I}
abstractNote = {A simple and sensitive solid-phase radioimmunoassay for the detection of immunoglobulins G, A and M in sucrose gradients is described. The solid-phase consisted of immunoglobulins adsorbed to polystyrene tubes. Using buffers without detergent and /sup 125/I-labeled sheep anti-rabbit IgA as radioligand, the assay was able to detect 0.8 ng per tube in the IgG assay and 1.6 ng per tube in the IgA and IgM assays. Standard curves with antigen dissolved in 10% and 32% sucrose were superimposable and did not deviate from standard curves with antigen dissolved in buffer without sucrose. Using these techniques on ultracentrifugation samples from patients with systemic lupus erythematosus, Schoenlein-Henoch nephritis and IgA glorulonephritis is was possible to detect both immunoglobulin fragments and immunoglobulin aggregates at the same time without prior dialysis of the samples.}
journal = {J. Immunol. Methods; (Netherlands)}
volume = {71:2}
journal type = {AC}
place = {Netherlands}
year = {1984}
month = {Jan}
}