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Effect of gonadotrophin inhibiting material isolated from human urine on action of prolactin at rat Leydig cell

Journal Article:

Abstract

To study the effect of gonadotropin inhibiting material (GIM) on the binding of prolactin to Leydig cell receptors isolated Leydig cells were incubated with sup(125)I-prolactin. Presence of GIM in the incubation mixture did not inhibit the binding of sup(125)I-prolactin to Leydig cells whereas unlabelled prolactin significantly reduced the binding. In another experiment, testicular cells were incubated with FITC-tagged GIM. Binding of GIM to Leydig cells was seen by the presence of fluorescence on these cells. This binding could be inhibited by untagged GIM but not by prolactin. The results suggest the presence of separate receptors for GIM and prolactin on the Leydig cells and indicate that termination of pregnancy by GIM is not due to any interference with prolactin binding to its receptors.
Authors:
Bagli, N P; Rajendran, K G; Shah, P N [1] 
  1. Cancer Research Inst., Bombay (India). Div. of Endocrinology
Publication Date:
May 01, 1980
Product Type:
Journal Article
Reference Number:
AIX-11-562170; EDB-81-112037
Resource Relation:
Journal Name: Indian J. Exp. Biol.; (India); Journal Volume: 18:5
Subject:
59 BASIC BIOLOGICAL SCIENCES; LTH; RECEPTORS; PREGNANCY; ANIMAL CELLS; CHEMICAL BONDS; GONADOTROPINS; IN VITRO; INHIBITION; IODINE 125; LABELLED COMPOUNDS; RATS; TESTES; TRACER TECHNIQUES; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; GONADS; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MALE GENITALS; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; ORGANS; PEPTIDE HORMONES; PITUITARY HORMONES; RADIOISOTOPES; RODENTS; VERTEBRATES; 551001* - Physiological Systems- Tracer Techniques
OSTI ID:
6230089
Country of Origin:
India
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: IJEBA
Submitting Site:
INIS
Size:
Pages: 456-458
Announcement Date:
Dec 01, 1980

Journal Article:

Citation Formats

Bagli, N P, Rajendran, K G, and Shah, P N. Effect of gonadotrophin inhibiting material isolated from human urine on action of prolactin at rat Leydig cell. India: N. p., 1980. Web.
Bagli, N P, Rajendran, K G, & Shah, P N. Effect of gonadotrophin inhibiting material isolated from human urine on action of prolactin at rat Leydig cell. India.
Bagli, N P, Rajendran, K G, and Shah, P N. 1980. "Effect of gonadotrophin inhibiting material isolated from human urine on action of prolactin at rat Leydig cell." India.
@misc{etde_6230089,
title = {Effect of gonadotrophin inhibiting material isolated from human urine on action of prolactin at rat Leydig cell}
author = {Bagli, N P, Rajendran, K G, and Shah, P N}
abstractNote = {To study the effect of gonadotropin inhibiting material (GIM) on the binding of prolactin to Leydig cell receptors isolated Leydig cells were incubated with sup(125)I-prolactin. Presence of GIM in the incubation mixture did not inhibit the binding of sup(125)I-prolactin to Leydig cells whereas unlabelled prolactin significantly reduced the binding. In another experiment, testicular cells were incubated with FITC-tagged GIM. Binding of GIM to Leydig cells was seen by the presence of fluorescence on these cells. This binding could be inhibited by untagged GIM but not by prolactin. The results suggest the presence of separate receptors for GIM and prolactin on the Leydig cells and indicate that termination of pregnancy by GIM is not due to any interference with prolactin binding to its receptors.}
journal = {Indian J. Exp. Biol.; (India)}
volume = {18:5}
journal type = {AC}
place = {India}
year = {1980}
month = {May}
}