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Radioimmunoassay of human cardiac tropomyosin in acute myocardial infarction

Journal Article:

Abstract

Tropomyosin was prepared from fresh human myocardium and antisera raised in rabbits. A sensitive radioimmunoassay was developed for the detection of human cardiac /sup 125/I-labelled tropomyosin in human sera down to levels of 1 ng/ml. Values for human cardiac tropomyosin in normal patients ranged from less than 1 to 3 ng/ml. In 18 patients with acute myocardial infarction all had elevated tropomyosin levels ranging from 41 to above 200 ng/ml with a mean peak level of 101 ng/ml. In this study there were no false positive or false negative results. In the initial stages of infarction the time course of appearance and peak levels of cardiac tropomyosin, total creatine kinase and creatine kinase MB isoenzyme were similar. Although total creatine kinase and creatine kinase MB isoenzyme levels were normal after 72 h in patients with single, uncomplicated infarction, cardiac tropomyosin levels were still significantly elevated above normal after this time, being 30-60% of peak valuctional hourly rate of absorption and the plasma /sup 32/P radioactivity at 60 min corrected for extracellular fluid volume provided the best app elements, the characteristics of which are determined by employing the Lagrange multiplier concept. Unknowns of the resulting simultaneous equation consist of usual nodal  More>>
Authors:
Cummins, P; McGurk, B; Littler, W A [1] 
  1. Queen Elizabeth Hospital, Birmingham (UK)
Publication Date:
Mar 01, 1981
Product Type:
Journal Article
Reference Number:
AIX-12-632607; EDB-81-123731
Resource Relation:
Journal Name: Clin. Sci.; (United Kingdom); Journal Volume: 60:3
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; MYOCARDIAL INFARCTION; DIAGNOSIS; MYOSIN; RADIOIMMUNOASSAY; BLOOD SERUM; IODINE 125; MYOCARDIUM; TIME DEPENDENCE; BETA DECAY RADIOISOTOPES; BODY; CARDIOVASCULAR DISEASES; CARDIOVASCULAR SYSTEM; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; GLOBULINS; HEART; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MUSCLES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOASSAY; RADIOISOTOPES; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
6222644
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: CSCIA
Submitting Site:
INIS
Size:
Pages: 251-259
Announcement Date:
Oct 01, 1981

Journal Article:

Citation Formats

Cummins, P, McGurk, B, and Littler, W A. Radioimmunoassay of human cardiac tropomyosin in acute myocardial infarction. United Kingdom: N. p., 1981. Web.
Cummins, P, McGurk, B, & Littler, W A. Radioimmunoassay of human cardiac tropomyosin in acute myocardial infarction. United Kingdom.
Cummins, P, McGurk, B, and Littler, W A. 1981. "Radioimmunoassay of human cardiac tropomyosin in acute myocardial infarction." United Kingdom.
@misc{etde_6222644,
title = {Radioimmunoassay of human cardiac tropomyosin in acute myocardial infarction}
author = {Cummins, P, McGurk, B, and Littler, W A}
abstractNote = {Tropomyosin was prepared from fresh human myocardium and antisera raised in rabbits. A sensitive radioimmunoassay was developed for the detection of human cardiac /sup 125/I-labelled tropomyosin in human sera down to levels of 1 ng/ml. Values for human cardiac tropomyosin in normal patients ranged from less than 1 to 3 ng/ml. In 18 patients with acute myocardial infarction all had elevated tropomyosin levels ranging from 41 to above 200 ng/ml with a mean peak level of 101 ng/ml. In this study there were no false positive or false negative results. In the initial stages of infarction the time course of appearance and peak levels of cardiac tropomyosin, total creatine kinase and creatine kinase MB isoenzyme were similar. Although total creatine kinase and creatine kinase MB isoenzyme levels were normal after 72 h in patients with single, uncomplicated infarction, cardiac tropomyosin levels were still significantly elevated above normal after this time, being 30-60% of peak valuctional hourly rate of absorption and the plasma /sup 32/P radioactivity at 60 min corrected for extracellular fluid volume provided the best app elements, the characteristics of which are determined by employing the Lagrange multiplier concept. Unknowns of the resulting simultaneous equation consist of usual nodal displacements of the whole stru element codes. Therefore, FAST should be useful in several areas for which all other codes are too unwieldy and expensivnt makers was established, in which the investigations and studies have started.}
journal = {Clin. Sci.; (United Kingdom)}
volume = {60:3}
journal type = {AC}
place = {United Kingdom}
year = {1981}
month = {Mar}
}