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Retinal uptake and release of (/sup 3/H)DABA

Journal Article:

Abstract

The uptake of (/sup 3/H)DABA was studied in rat, guinea-pig and cat retinas in vivo and in rabbit retinas both in vivo and in vitro by autoradiography. (/sup 3/H)DABA was preferentially accumulated by a type of amacrine cell but after brief incubation or shortly after intravitreal injections there was not only a neuronal uptake but also a glial one. In guinea-pigs the glial labelling in vivo was significant also after 4 hr. The glial uptake (rabbits) was found to be saturable and temperature dependent as expected for an active uptake mechanism. The Ksub(m) of DABA uptake was 2.11 x 10/sup -5/ m and Vsub(max) 2.38 x 10/sup -5/ mol/mg/min. GABA competitively inhibited the DABA uptake. The uptake was not statistically significantly influenced by OMEGA-amino acids such as glycine, ..beta..-alanine, ..cap alpha..-alanine or epsilon-aminocaproic acid. The effect of different stimuli on the spontaneous efflux of radioactivity from (/sup 3/H)DABA preloaded rabbit retinas was studied with (/sup 3/H)DABA localized to neurons. Light flashes evoked a small but not statistically significant increased release whereas 40mM-K/sup +/ evoked an immediate and large increase. Unlabelled DABA, GABA and ..beta..-alanine (10/sup -5/M) increased the spontaneous efflux of (/sup 3/H)DABA but not glycine. It is concluded that  More>>
Authors:
Bauer, B; Ehinger, B [1] 
  1. Lund Univ. (Sweden)
Publication Date:
Mar 01, 1978
Product Type:
Journal Article
Reference Number:
AIX-10-420935; EDB-79-051990
Resource Relation:
Journal Name: Exp. Eye Res.; (United Kingdom); Journal Volume: 26:3
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; AMINOBUTYRIC ACID; BIOLOGICAL LOCALIZATION; RETINA; SPECIFICITY; TRITIUM COMPOUNDS; UPTAKE; CATS; COMPARATIVE EVALUATIONS; GUINEA PIGS; LABELLED COMPOUNDS; NERVE CELLS; POTASSIUM COMPOUNDS; RABBITS; RADIONUCLIDE KINETICS; RATS; RESPONSE MODIFYING FACTORS; TEMPERATURE DEPENDENCE; TRACER TECHNIQUES; ALKALI METAL COMPOUNDS; AMINO ACIDS; ANIMAL CELLS; ANIMALS; BODY; CARBOXYLIC ACIDS; EYES; ISOTOPE APPLICATIONS; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; RODENTS; SENSE ORGANS; SOMATIC CELLS; VERTEBRATES; 560172* - Radiation Effects- Nuclide Kinetics & Toxicology- Animals- (-1987); 551000 - Physiological Systems
OSTI ID:
6201859
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: EXERA
Submitting Site:
INIS
Size:
Pages: 275-289
Announcement Date:

Journal Article:

Citation Formats

Bauer, B, and Ehinger, B. Retinal uptake and release of (/sup 3/H)DABA. United Kingdom: N. p., 1978. Web.
Bauer, B, & Ehinger, B. Retinal uptake and release of (/sup 3/H)DABA. United Kingdom.
Bauer, B, and Ehinger, B. 1978. "Retinal uptake and release of (/sup 3/H)DABA." United Kingdom.
@misc{etde_6201859,
title = {Retinal uptake and release of (/sup 3/H)DABA}
author = {Bauer, B, and Ehinger, B}
abstractNote = {The uptake of (/sup 3/H)DABA was studied in rat, guinea-pig and cat retinas in vivo and in rabbit retinas both in vivo and in vitro by autoradiography. (/sup 3/H)DABA was preferentially accumulated by a type of amacrine cell but after brief incubation or shortly after intravitreal injections there was not only a neuronal uptake but also a glial one. In guinea-pigs the glial labelling in vivo was significant also after 4 hr. The glial uptake (rabbits) was found to be saturable and temperature dependent as expected for an active uptake mechanism. The Ksub(m) of DABA uptake was 2.11 x 10/sup -5/ m and Vsub(max) 2.38 x 10/sup -5/ mol/mg/min. GABA competitively inhibited the DABA uptake. The uptake was not statistically significantly influenced by OMEGA-amino acids such as glycine, ..beta..-alanine, ..cap alpha..-alanine or epsilon-aminocaproic acid. The effect of different stimuli on the spontaneous efflux of radioactivity from (/sup 3/H)DABA preloaded rabbit retinas was studied with (/sup 3/H)DABA localized to neurons. Light flashes evoked a small but not statistically significant increased release whereas 40mM-K/sup +/ evoked an immediate and large increase. Unlabelled DABA, GABA and ..beta..-alanine (10/sup -5/M) increased the spontaneous efflux of (/sup 3/H)DABA but not glycine. It is concluded that there is in the retina of rats, guinea-pigs, cats, and rabbits, a glial high affinity uptake mechanism in addition to the neuronal uptake. DABA seems to be transported by the same mechanism as GABA in both systems. The DABA seems to be better retained in neurons than in glia in rats, cats and rabbits, which allows it to be used as a neuronal marker.}
journal = {Exp. Eye Res.; (United Kingdom)}
volume = {26:3}
journal type = {AC}
place = {United Kingdom}
year = {1978}
month = {Mar}
}