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Effect of parenteral glutamate treatment on the localization of neurotransmitters in the mediobasal hypothalamus

Journal Article:

Abstract

The localization of cholinergic, aminergic and amino acid-ergic neurones in the mediobasal hypothalamus has been studied in normal rat brain and in brains where neurones in nucleus arcuatus were destroyed by repeated administration of 2 mg/g body weight monosodium glutamate to newborn animals. In normal animals acetylcholinesterase staining, choline acetyltransferase and aromatic L-amino acid decarboxylase were concentrated in the median eminence and the arcuate nucleus. Glutamate decarboxylase was concentrated at the boundary between the ventromedial and the arcuate nuclei, with lower activity in the arcuate nucleus and very low activity in the median eminence. Nucleus arcuatus contained an intermediate level of high affinity glutamate uptake. In the lesioned animals, there were significant decreases in choline acetyltransferase, acetylcholinesterase staining and glutamate decarboxylase in the median eminence, whereas choline acetyltransferase activity and acetylcholinesterase staining, but not glutamate decarboxylase activity, were decreased in nucleus arcuatus. Aromatic L-amino acid decarboxylase was unchanged in all regions studied. The high affinity uptakes of glutamate, dopamine and noradrenaline, and the endogenous amino acid levels were also unchanged in the treated animals. The results indicate the existence of acetylcholine- and GABA-containing elements in the tuberoinfundibular tract. They further indicate that the dopamine cells in the arcuate nucleus are  More>>
Publication Date:
Jan 01, 1978
Product Type:
Journal Article
Reference Number:
EDB-83-078759
Resource Relation:
Journal Name: Brain Res.; (Netherlands); Journal Volume: 153
Subject:
59 BASIC BIOLOGICAL SCIENCES; GLUTAMIC ACID; BIOLOGICAL EFFECTS; HYPOTHALAMUS; MORPHOLOGY; CARBON 14 COMPOUNDS; DECARBOXYLASES; NEONATES; NERVE CELLS; NEUROLOGY; RATS; RECEPTORS; TRACER TECHNIQUES; TRANSFERASES; TRITIUM COMPOUNDS; AMINO ACIDS; ANIMAL CELLS; ANIMALS; BODY; BRAIN; CARBON-CARBON LYASES; CARBOXY-LYASES; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM; ENZYMES; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LYASES; MAMMALS; MEDICINE; NERVOUS SYSTEM; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; RODENTS; SOMATIC CELLS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques; 550801 - Morphology- Tracer Techniques
OSTI ID:
6186843
Research Organizations:
Norwegian Defence Research Establishment, Kjeller
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BRREA
Submitting Site:
HEDB
Size:
Pages: 549-562
Announcement Date:

Journal Article:

Citation Formats

Walaas, I, and Fonnum, F. Effect of parenteral glutamate treatment on the localization of neurotransmitters in the mediobasal hypothalamus. Netherlands: N. p., 1978. Web.
Walaas, I, & Fonnum, F. Effect of parenteral glutamate treatment on the localization of neurotransmitters in the mediobasal hypothalamus. Netherlands.
Walaas, I, and Fonnum, F. 1978. "Effect of parenteral glutamate treatment on the localization of neurotransmitters in the mediobasal hypothalamus." Netherlands.
@misc{etde_6186843,
title = {Effect of parenteral glutamate treatment on the localization of neurotransmitters in the mediobasal hypothalamus}
author = {Walaas, I, and Fonnum, F}
abstractNote = {The localization of cholinergic, aminergic and amino acid-ergic neurones in the mediobasal hypothalamus has been studied in normal rat brain and in brains where neurones in nucleus arcuatus were destroyed by repeated administration of 2 mg/g body weight monosodium glutamate to newborn animals. In normal animals acetylcholinesterase staining, choline acetyltransferase and aromatic L-amino acid decarboxylase were concentrated in the median eminence and the arcuate nucleus. Glutamate decarboxylase was concentrated at the boundary between the ventromedial and the arcuate nuclei, with lower activity in the arcuate nucleus and very low activity in the median eminence. Nucleus arcuatus contained an intermediate level of high affinity glutamate uptake. In the lesioned animals, there were significant decreases in choline acetyltransferase, acetylcholinesterase staining and glutamate decarboxylase in the median eminence, whereas choline acetyltransferase activity and acetylcholinesterase staining, but not glutamate decarboxylase activity, were decreased in nucleus arcuatus. Aromatic L-amino acid decarboxylase was unchanged in all regions studied. The high affinity uptakes of glutamate, dopamine and noradrenaline, and the endogenous amino acid levels were also unchanged in the treated animals. The results indicate the existence of acetylcholine- and GABA-containing elements in the tuberoinfundibular tract. They further indicate that the dopamine cells in the arcuate nucleus are less sensitive to the toxic effect of glutamate than other cell types, possibly because they contain less glutamate receptors.}
journal = {Brain Res.; (Netherlands)}
volume = {153}
journal type = {AC}
place = {Netherlands}
year = {1978}
month = {Jan}
}