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Effect of nalidixic acid on repair of single-strand breaks in DNA induced by ionizing irradiation in Escherichia coli

Journal Article:

Abstract

The incidence of DNA single-strand breaks induced by /sup 60/Co irradiation and their repair in E.coli K12 (AB 1157) rec/sup +/ cells were studied by the alkaline sucrose gradient sedimentation method described by McGrath and Williams. For the quantitative analysis of sedimentation profiles we used the s 1/2 values described by Veatch and Okada. The s 1/2 value of non-irradiated controls was 22.4, and after 20 krads irradiation it was found to be 11.7. A postirradiation incubation at 37 /sup 0/C for 60 min increasedthe s 1/2 value from 11.7 to 22.1. Nalidixic acid at low concentration (20-50 ..mu..g/ml) did not block, but at 100 ..mu..g/ml extensively inhibited the above repair process, exhibiting an s 1/2 value of 14.4.
Authors:
Francia, I; [1]  Okos, A; Hernadi, F J [2] 
  1. Debreceni Orvostudomanyi Egyetem (Hungary)
  2. Institute of Pharmacology, Debrecen (Hungary)
Publication Date:
Sep 30, 1978
Product Type:
Journal Article
Reference Number:
AIX-10-448535; EDB-79-075396
Resource Relation:
Journal Name: Stud. Biophys.; (German Democratic Republic); Journal Volume: 71:3
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; COBALT 60; BIOLOGICAL RADIATION EFFECTS; DNA; BIOLOGICAL REPAIR; ANTIBIOTICS; ESCHERICHIA COLI; GAMMA RADIATION; INHIBITION; STRAND BREAKS; BACTERIA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; COBALT ISOTOPES; DRUGS; ELECTROMAGNETIC RADIATION; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MICROORGANISMS; MINUTES LIVING RADIOISOTOPES; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; RECOVERY; REPAIR; YEARS LIVING RADIOISOTOPES; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
OSTI ID:
6179928
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: STBIB
Submitting Site:
INIS
Size:
Pages: 143-149
Announcement Date:

Journal Article:

Citation Formats

Francia, I, Okos, A, and Hernadi, F J. Effect of nalidixic acid on repair of single-strand breaks in DNA induced by ionizing irradiation in Escherichia coli. Germany: N. p., 1978. Web.
Francia, I, Okos, A, & Hernadi, F J. Effect of nalidixic acid on repair of single-strand breaks in DNA induced by ionizing irradiation in Escherichia coli. Germany.
Francia, I, Okos, A, and Hernadi, F J. 1978. "Effect of nalidixic acid on repair of single-strand breaks in DNA induced by ionizing irradiation in Escherichia coli." Germany.
@misc{etde_6179928,
title = {Effect of nalidixic acid on repair of single-strand breaks in DNA induced by ionizing irradiation in Escherichia coli}
author = {Francia, I, Okos, A, and Hernadi, F J}
abstractNote = {The incidence of DNA single-strand breaks induced by /sup 60/Co irradiation and their repair in E.coli K12 (AB 1157) rec/sup +/ cells were studied by the alkaline sucrose gradient sedimentation method described by McGrath and Williams. For the quantitative analysis of sedimentation profiles we used the s 1/2 values described by Veatch and Okada. The s 1/2 value of non-irradiated controls was 22.4, and after 20 krads irradiation it was found to be 11.7. A postirradiation incubation at 37 /sup 0/C for 60 min increasedthe s 1/2 value from 11.7 to 22.1. Nalidixic acid at low concentration (20-50 ..mu..g/ml) did not block, but at 100 ..mu..g/ml extensively inhibited the above repair process, exhibiting an s 1/2 value of 14.4.}
journal = {Stud. Biophys.; (German Democratic Republic)}
volume = {71:3}
journal type = {AC}
place = {Germany}
year = {1978}
month = {Sep}
}