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Studies on insulin receptor, 2. Studies on the influence of starvation and high fat diet on insulin receptor

Journal Article:

Abstract

The present study is to investigate an influence of starvation and high fat diet on insulin receptor of the plasma membrane by means of radioreceptor assay using /sup 125/I-labelled insulin. Male guinea pigs of Hartley strain were employed for the starvation study, and /sup 125/I-insulin binding capacity on the plasma membrane of the liver and kidney was determined at 24, 48 and 72 hours of the fast after the last meal. Male rats of Wistar strain were employed for the high fat study where the diet containing 35% of butter was fed ad libitum for 38 or 68 days. The animals were killed at the fast of 12 hours, and /sup 125/I-insulin binding capacity on the plasma membrane of the liver was determined. The results obtained are summarized as follows: 1) An increase in /sup 125/I-insulin binding capacity on the plasma membrane of the liver and kidney was observed by the starvation for 24 to 72 hours. 2) The mechanism of the increase by starvation was considered to be different by the organs; it was due to an increase in number of insulin receptor in the liver, and due to an increase in affinity of insulin receptor in the kidney.  More>>
Authors:
Sakai, Y [1] 
  1. Hiroshima Univ. (Japan). School of Medicine
Publication Date:
Aug 01, 1979
Product Type:
Journal Article
Reference Number:
AIX-11-562167; EDB-81-111918
Resource Relation:
Journal Name: Med. J. Hiroshima Univ.; (Japan); Journal Volume: 27:4
Subject:
59 BASIC BIOLOGICAL SCIENCES; FASTING; BIOLOGICAL EFFECTS; BIOLOGICAL PATHWAYS; RECEPTORS; INSULIN; RADIOASSAY; AFFINITY; CARBOHYDRATES; DIET; FATS; GUINEA PIGS; IODINE 125; KIDNEYS; LIVER; METABOLISM; NUTRIENTS; NUTRITION; PROTEINS; RATS; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; ELECTRON CAPTURE RADIOISOTOPES; GLANDS; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; RADIOISOTOPES; RODENTS; VERTEBRATES; 550501* - Metabolism- Tracer Techniques; 551001 - Physiological Systems- Tracer Techniques
OSTI ID:
6163327
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: CODEN: HDIZA
Submitting Site:
INIS
Size:
Pages: 511-525
Announcement Date:

Journal Article:

Citation Formats

Sakai, Y. Studies on insulin receptor, 2. Studies on the influence of starvation and high fat diet on insulin receptor. Japan: N. p., 1979. Web.
Sakai, Y. Studies on insulin receptor, 2. Studies on the influence of starvation and high fat diet on insulin receptor. Japan.
Sakai, Y. 1979. "Studies on insulin receptor, 2. Studies on the influence of starvation and high fat diet on insulin receptor." Japan.
@misc{etde_6163327,
title = {Studies on insulin receptor, 2. Studies on the influence of starvation and high fat diet on insulin receptor}
author = {Sakai, Y}
abstractNote = {The present study is to investigate an influence of starvation and high fat diet on insulin receptor of the plasma membrane by means of radioreceptor assay using /sup 125/I-labelled insulin. Male guinea pigs of Hartley strain were employed for the starvation study, and /sup 125/I-insulin binding capacity on the plasma membrane of the liver and kidney was determined at 24, 48 and 72 hours of the fast after the last meal. Male rats of Wistar strain were employed for the high fat study where the diet containing 35% of butter was fed ad libitum for 38 or 68 days. The animals were killed at the fast of 12 hours, and /sup 125/I-insulin binding capacity on the plasma membrane of the liver was determined. The results obtained are summarized as follows: 1) An increase in /sup 125/I-insulin binding capacity on the plasma membrane of the liver and kidney was observed by the starvation for 24 to 72 hours. 2) The mechanism of the increase by starvation was considered to be different by the organs; it was due to an increase in number of insulin receptor in the liver, and due to an increase in affinity of insulin receptor in the kidney. 3) In non-obese rats fed with high fat diet, the number of insulin receptor on the liver plasma membrane showed a decrease, and this observation clearly indicated that the decrease in number of the receptor did not depend on the obesity. 4) Obese rats also fed with high fat diet presented a decrease in number of insulin receptor without an elevation of insulin levels in the circulating blood. This indicated that at least in the obese rats fed with high fat diet, the decrease in number of the receptor was not due to hyperinsulinemia.}
journal = {Med. J. Hiroshima Univ.; (Japan)}
volume = {27:4}
journal type = {AC}
place = {Japan}
year = {1979}
month = {Aug}
}