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Method of preparing methyl-alpha-D-glucopyranoside labelled with /sup 14/C radioisotope or /sup 13/C stable isotope, using labelled alpha-D-glucan. Zpusob pripravy methyl-alfa-D-glukopyranosidu znaceneho radioizotopem /sup 14/C nebo stabilnim izotopem /sup 13/C s vyuzitim znaceneho alfa-D-glukanu

Abstract

Labelled ..cap alpha..-D-glucan separated from photoautotrophic organisms is introduced into a reaction with 5 to 25% methanolic trifluoroacetic acid in the presence of 2-4% water related to the weight of the methanol. The reaction takes place in a closed system at a temperature of 85 to 100 degC over a period of 4 to 24 hours. Examples are given of the preparation of methyl-..cap alpha..-D-glucopyranoside under various reaction conditions. The use of trifluoroacetic acid at acidic methanolysis of ..cap alpha..-D-glucan is kinder than the use of the usual mineral acids. The processing of the mixture following the reaction is simpler, too - trifluoroacetic acid may be removed by vacuum distillation. Another advantage of the method is the reduction of losses connected with the decomposition of D-glucose and the product and with the processing of the reaction mixture. Labelled methanol may be used instead of labelled ..cap alpha..-D-glucan.
Authors:
Publication Date:
Jun 15, 1986
Product Type:
Patent
Report Number:
CS 231499; B
Reference Number:
EDB-87-125350
Resource Relation:
Patent File Date: Filed date 5 Jul 1983; Other Information: CS patent application PV 5098-83
Subject:
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY; CARBON 13; ISOTOPIC EXCHANGE; CARBON 14; GLYCOSIDES; CHEMICAL PREPARATION; LABELLING; LABELLED COMPOUNDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOHYDRATES; CARBON ISOTOPES; EVEN-EVEN NUCLEI; EVEN-ODD NUCLEI; ISOTOPES; LIGHT NUCLEI; NUCLEI; ORGANIC COMPOUNDS; RADIOISOTOPES; STABLE ISOTOPES; SYNTHESIS; YEARS LIVING RADIOISOTOPES; 400703* - Radiochemistry & Nuclear Chemistry- Radioisotope Production
OSTI ID:
6156498
Country of Origin:
Serbia and Montenegro
Language:
Czech
Submitting Site:
INIS
Size:
Pages: 3
Announcement Date:
May 13, 2001

Citation Formats

Skala, L. Method of preparing methyl-alpha-D-glucopyranoside labelled with /sup 14/C radioisotope or /sup 13/C stable isotope, using labelled alpha-D-glucan. Zpusob pripravy methyl-alfa-D-glukopyranosidu znaceneho radioizotopem /sup 14/C nebo stabilnim izotopem /sup 13/C s vyuzitim znaceneho alfa-D-glukanu. Serbia and Montenegro: N. p., 1986. Web.
Skala, L. Method of preparing methyl-alpha-D-glucopyranoside labelled with /sup 14/C radioisotope or /sup 13/C stable isotope, using labelled alpha-D-glucan. Zpusob pripravy methyl-alfa-D-glukopyranosidu znaceneho radioizotopem /sup 14/C nebo stabilnim izotopem /sup 13/C s vyuzitim znaceneho alfa-D-glukanu. Serbia and Montenegro.
Skala, L. 1986. "Method of preparing methyl-alpha-D-glucopyranoside labelled with /sup 14/C radioisotope or /sup 13/C stable isotope, using labelled alpha-D-glucan. Zpusob pripravy methyl-alfa-D-glukopyranosidu znaceneho radioizotopem /sup 14/C nebo stabilnim izotopem /sup 13/C s vyuzitim znaceneho alfa-D-glukanu." Serbia and Montenegro.
@misc{etde_6156498,
title = {Method of preparing methyl-alpha-D-glucopyranoside labelled with /sup 14/C radioisotope or /sup 13/C stable isotope, using labelled alpha-D-glucan. Zpusob pripravy methyl-alfa-D-glukopyranosidu znaceneho radioizotopem /sup 14/C nebo stabilnim izotopem /sup 13/C s vyuzitim znaceneho alfa-D-glukanu}
author = {Skala, L}
abstractNote = {Labelled ..cap alpha..-D-glucan separated from photoautotrophic organisms is introduced into a reaction with 5 to 25% methanolic trifluoroacetic acid in the presence of 2-4% water related to the weight of the methanol. The reaction takes place in a closed system at a temperature of 85 to 100 degC over a period of 4 to 24 hours. Examples are given of the preparation of methyl-..cap alpha..-D-glucopyranoside under various reaction conditions. The use of trifluoroacetic acid at acidic methanolysis of ..cap alpha..-D-glucan is kinder than the use of the usual mineral acids. The processing of the mixture following the reaction is simpler, too - trifluoroacetic acid may be removed by vacuum distillation. Another advantage of the method is the reduction of losses connected with the decomposition of D-glucose and the product and with the processing of the reaction mixture. Labelled methanol may be used instead of labelled ..cap alpha..-D-glucan.}
place = {Serbia and Montenegro}
year = {1986}
month = {Jun}
}