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Radioiodine labelling of insulin using dimethylsulfoxide as a labelling-aid

Journal Article:

Abstract

Using dimethylsulfoxide (DMSO) as a labelling aid, insulin /sup 126/I of radioimmunoassay use has been effectively prepared. A small amount of DMSO was added to usual labelling mixture and the reaction time was controlled. The labelled insulin obtained in such a way showed improved bindabilities to the antibody and thus expressed larger dose-gradients in the plots of standard dose-response curves even though the labelling rate was decreased to some extent. However, by extending the reaction time to about 1 min, average labelling yield of 30% could be obtained. The average increase of bindability (B/F) in definite antiserum dilution was 2.5 compared with 1.5 obtained in the absence of DMSO. Thus, the net bindability increase was 70% of those obtained in the absence of DMSO. By means of NMR spectrometry, it has been confirmed that the DMSO in the labelling mixture is converted to dimethylsulfone by chloramine-T. The results, generally agreed with the Stagg's postulation, were discussed in view of a competitive oxidation of DMSO with disulfide linkages of the insulin molecule by the chloramine-T.
Authors:
Kim, J; Kim, Y H [1] 
  1. Korea Atomic Energy Research Inst., Seoul, (Republic of Korea)
Publication Date:
Dec 01, 1977
Product Type:
Journal Article
Reference Number:
AIX-10-425670; EDB-79-069645
Resource Relation:
Journal Name: J. Korean Nucl. Soc.; (Korea, South); Journal Volume: 9:4
Subject:
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY; INSULIN; LABELLING; IODINE 126; RADIOCHEMISTRY; DMSO; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; CHEMISTRY; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HORMONES; HOURS LIVING RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PEPTIDE HORMONES; RADIOISOTOPES; SULFOXIDES; 400702* - Radiochemistry & Nuclear Chemistry- Properties of Radioactive Materials
OSTI ID:
6141098
Country of Origin:
Korea, Republic of
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: KNSJA
Submitting Site:
INIS
Size:
Pages: 203-210
Announcement Date:

Journal Article:

Citation Formats

Kim, J, and Kim, Y H. Radioiodine labelling of insulin using dimethylsulfoxide as a labelling-aid. Korea, Republic of: N. p., 1977. Web.
Kim, J, & Kim, Y H. Radioiodine labelling of insulin using dimethylsulfoxide as a labelling-aid. Korea, Republic of.
Kim, J, and Kim, Y H. 1977. "Radioiodine labelling of insulin using dimethylsulfoxide as a labelling-aid." Korea, Republic of.
@misc{etde_6141098,
title = {Radioiodine labelling of insulin using dimethylsulfoxide as a labelling-aid}
author = {Kim, J, and Kim, Y H}
abstractNote = {Using dimethylsulfoxide (DMSO) as a labelling aid, insulin /sup 126/I of radioimmunoassay use has been effectively prepared. A small amount of DMSO was added to usual labelling mixture and the reaction time was controlled. The labelled insulin obtained in such a way showed improved bindabilities to the antibody and thus expressed larger dose-gradients in the plots of standard dose-response curves even though the labelling rate was decreased to some extent. However, by extending the reaction time to about 1 min, average labelling yield of 30% could be obtained. The average increase of bindability (B/F) in definite antiserum dilution was 2.5 compared with 1.5 obtained in the absence of DMSO. Thus, the net bindability increase was 70% of those obtained in the absence of DMSO. By means of NMR spectrometry, it has been confirmed that the DMSO in the labelling mixture is converted to dimethylsulfone by chloramine-T. The results, generally agreed with the Stagg's postulation, were discussed in view of a competitive oxidation of DMSO with disulfide linkages of the insulin molecule by the chloramine-T.}
journal = {J. Korean Nucl. Soc.; (Korea, South)}
volume = {9:4}
journal type = {AC}
place = {Korea, Republic of}
year = {1977}
month = {Dec}
}