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''reverse'' solid-phase radioimmunoasssay for IgM-antibodies to hepatitis A virus

Journal Article:

Abstract

A ''reverse'' solid-phase radio-immuno-assay for IgM antibodies to hepatitis A virus (HAV) was developed. Anti-human IgM immunoglobulins were bound on the wells of polyvinylchloride microtiter plates. Serum specimens were incubated in the anti-human IgM coated wells and bound IgM antibodies were then assayed for antigen specificity by subsequent incubations with HAV antigen and /sup 125/I-labelled human anti-HAV IgG. The test showed a high sensitivity and specificity for anti-HAV IgM antibodies. No false-positive reactions were observed either in the sera from patients with hepatobiliary disorders other than HAV infection or in the sera containing both rheumatoid factor and anti-HAV IgG antibodies. In acute HAV infections specific IgM antibodies were present already in the first specimens taken within a few days after the onset of jaundice. The persistence of the IgM antibodies was from 4 to 6 months. IgM antibody titers up to 1,000,000 were observed in the acute phase of HAV infection. In routine diagnostic work the titration of the sera was not necessary, since a reliable qualitative result was obtained by testing the sera in a single dilution of 1:100. A similar reverse immuno-assay principle may be adaptable for the diagnostic determination of IgM antibodies to different viral and microbial  More>>
Authors:
Meurman, O H; Matter, L; Krishna, R V; Krech, U H [1] 
  1. Institute of Medical Microbiology, St. Gallen, Switzerland
Publication Date:
Jan 01, 1981
Product Type:
Journal Article
Reference Number:
AIX-12-616029; EDB-82-005081
Resource Relation:
Journal Name: Acta Pathol. Microbiol. Scand., Sect. C; (Denmark); Journal Volume: 89
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANTIBODIES; RADIOIMMUNOASSAY; HEPATITIS; DIAGNOSTIC TECHNIQUES; SPECIFICITY; VIRUSES; DIGESTIVE SYSTEM DISEASES; DISEASES; ISOTOPE APPLICATIONS; MICROORGANISMS; PARASITES; RADIOASSAY; TRACER TECHNIQUES; 550901* - Pathology- Tracer Techniques
OSTI ID:
6119816
Country of Origin:
Denmark
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: APSCD
Submitting Site:
INIS
Size:
Pages: 79-84
Announcement Date:
Aug 01, 1981

Journal Article:

Citation Formats

Meurman, O H, Matter, L, Krishna, R V, and Krech, U H. ''reverse'' solid-phase radioimmunoasssay for IgM-antibodies to hepatitis A virus. Denmark: N. p., 1981. Web.
Meurman, O H, Matter, L, Krishna, R V, & Krech, U H. ''reverse'' solid-phase radioimmunoasssay for IgM-antibodies to hepatitis A virus. Denmark.
Meurman, O H, Matter, L, Krishna, R V, and Krech, U H. 1981. "''reverse'' solid-phase radioimmunoasssay for IgM-antibodies to hepatitis A virus." Denmark.
@misc{etde_6119816,
title = {''reverse'' solid-phase radioimmunoasssay for IgM-antibodies to hepatitis A virus}
author = {Meurman, O H, Matter, L, Krishna, R V, and Krech, U H}
abstractNote = {A ''reverse'' solid-phase radio-immuno-assay for IgM antibodies to hepatitis A virus (HAV) was developed. Anti-human IgM immunoglobulins were bound on the wells of polyvinylchloride microtiter plates. Serum specimens were incubated in the anti-human IgM coated wells and bound IgM antibodies were then assayed for antigen specificity by subsequent incubations with HAV antigen and /sup 125/I-labelled human anti-HAV IgG. The test showed a high sensitivity and specificity for anti-HAV IgM antibodies. No false-positive reactions were observed either in the sera from patients with hepatobiliary disorders other than HAV infection or in the sera containing both rheumatoid factor and anti-HAV IgG antibodies. In acute HAV infections specific IgM antibodies were present already in the first specimens taken within a few days after the onset of jaundice. The persistence of the IgM antibodies was from 4 to 6 months. IgM antibody titers up to 1,000,000 were observed in the acute phase of HAV infection. In routine diagnostic work the titration of the sera was not necessary, since a reliable qualitative result was obtained by testing the sera in a single dilution of 1:100. A similar reverse immuno-assay principle may be adaptable for the diagnostic determination of IgM antibodies to different viral and microbial antigens.}
journal = {Acta Pathol. Microbiol. Scand., Sect. C; (Denmark)}
volume = {89}
journal type = {AC}
place = {Denmark}
year = {1981}
month = {Jan}
}