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Technetium-99m-labeled recombinant tissue plasminogen activator for the imaging of emboli in vivo

Journal Article:

Abstract

Tissue-type plasminogen activator (t-PA) effectively lyses activate thrombus by direct action. Recombinant t-PA (rt-PA) was labeled with technetium-99m ([sup 99m]Tc) to investigate the in vivo binding to fibrin clots in a feline cerebral embolism model created by insertion of an artificial fibrin clot within the carotid artery. [sup 99m]Tc-rt-PA administered intravenously provided clearer imaging of clots after priming with cold rt-PA, with uptake peaking 5-10 minutes after the injection. [sup 99m]Tc-labeled human serum albumin was not retained at clot sites. Systemically administered [sup 99m]Tc-rt-PA binds to fibrin clots within carotid arteries in our feline model. Our results suggest that the interaction of intrinsic plasminogen activator inhibitors with extrinsically administered rt-PA may regulate the demonstration of a clot, although the precise mechanism is unclear. (author).
Authors:
Takahashi, Akihiro; Itoh, Kazuo; Tsukamoto, Eriko; Furudate, Masayori; Kamiyama, Hiroyasu; Abe, Hiroshi [1] 
  1. Hokkaido Univ., Sapporo (Japan). School of Medicine
Publication Date:
Jul 01, 1993
Product Type:
Journal Article
Reference Number:
JPN-93-009257; EDB-93-137082
Resource Relation:
Journal Name: Neurologia Medico-Chirurgica; (Japan); Journal Volume: 33:7
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; CAROTID ARTERIES; SCINTISCANNING; PLASMINOGEN; LABELLING; TECHNETIUM 99; AFFINITY; ALBUMINS; CATS; CEREBRUM; EMBOLI; FIBRIN; THROMBOSIS; UPTAKE; ANIMALS; ARTERIES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BLOOD COAGULATION FACTORS; BLOOD VESSELS; BODY; BRAIN; CARDIOVASCULAR DISEASES; CARDIOVASCULAR SYSTEM; CENTRAL NERVOUS SYSTEM; COAGULANTS; COUNTING TECHNIQUES; DIAGNOSTIC TECHNIQUES; DISEASES; DRUGS; FIBRINOLYTIC AGENTS; HEMATOLOGIC AGENTS; HEMOSTATICS; HOURS LIVING RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MAMMALS; NERVOUS SYSTEM; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOISOTOPE SCANNING; RADIOISOTOPES; SCLEROPROTEINS; TECHNETIUM ISOTOPES; VASCULAR DISEASES; VERTEBRATES; YEARS LIVING RADIOI; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
6117904
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0387-2572; CODEN: NMCHBN
Submitting Site:
JPN
Size:
Pages: 415-419
Announcement Date:
Nov 01, 1993

Journal Article:

Citation Formats

Takahashi, Akihiro, Itoh, Kazuo, Tsukamoto, Eriko, Furudate, Masayori, Kamiyama, Hiroyasu, and Abe, Hiroshi. Technetium-99m-labeled recombinant tissue plasminogen activator for the imaging of emboli in vivo. Japan: N. p., 1993. Web.
Takahashi, Akihiro, Itoh, Kazuo, Tsukamoto, Eriko, Furudate, Masayori, Kamiyama, Hiroyasu, & Abe, Hiroshi. Technetium-99m-labeled recombinant tissue plasminogen activator for the imaging of emboli in vivo. Japan.
Takahashi, Akihiro, Itoh, Kazuo, Tsukamoto, Eriko, Furudate, Masayori, Kamiyama, Hiroyasu, and Abe, Hiroshi. 1993. "Technetium-99m-labeled recombinant tissue plasminogen activator for the imaging of emboli in vivo." Japan.
@misc{etde_6117904,
title = {Technetium-99m-labeled recombinant tissue plasminogen activator for the imaging of emboli in vivo}
author = {Takahashi, Akihiro, Itoh, Kazuo, Tsukamoto, Eriko, Furudate, Masayori, Kamiyama, Hiroyasu, and Abe, Hiroshi}
abstractNote = {Tissue-type plasminogen activator (t-PA) effectively lyses activate thrombus by direct action. Recombinant t-PA (rt-PA) was labeled with technetium-99m ([sup 99m]Tc) to investigate the in vivo binding to fibrin clots in a feline cerebral embolism model created by insertion of an artificial fibrin clot within the carotid artery. [sup 99m]Tc-rt-PA administered intravenously provided clearer imaging of clots after priming with cold rt-PA, with uptake peaking 5-10 minutes after the injection. [sup 99m]Tc-labeled human serum albumin was not retained at clot sites. Systemically administered [sup 99m]Tc-rt-PA binds to fibrin clots within carotid arteries in our feline model. Our results suggest that the interaction of intrinsic plasminogen activator inhibitors with extrinsically administered rt-PA may regulate the demonstration of a clot, although the precise mechanism is unclear. (author).}
journal = {Neurologia Medico-Chirurgica; (Japan)}
volume = {33:7}
journal type = {AC}
place = {Japan}
year = {1993}
month = {Jul}
}