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Two-site immunoradiometric assay for the MB isoenzyme of creatine kinase

Abstract

A two-site immunoradiometric assay for myocardial creatine kinase MB isoenzyme is described. The method utilizes immobilized anti-human creatine kinase BB antibodies and /sup 125/I-labelled anti-human creatine kinase MM antibodies and can specifically detect creatine kinase MB in the presence of approximately 1000-fold excess of creatine kinase MM or BB. Native kinase MB prepared from human heart and creatine kinase MB prepared by hybridisation of purified human creatine kinase MM and creatine kinase BB appeared to react identically in the assay. Serum estimations on patients with suspected myocardial infarction correlated with the presence of MB band on electrophoresis but preliminary results suggest that the two-site immunoradiometric assay may be more sensitive.
Authors:
Willson, V J.C.; Jones, H M; Thompson, R J [1] 
  1. Cambridge Univ. (UK). Clinical School
Publication Date:
Jun 18, 1981
Product Type:
Journal Article
Reference Number:
AIX-12-629583; EDB-81-131675
Resource Relation:
Journal Name: Clin. Chim. Acta; (Netherlands); Journal Volume: 113:2
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; CREATINE; PHOSPHOTRANSFERASES; ISOENZYMES; RADIOIMMUNOASSAY; BIOCHEMICAL REACTION KINETICS; CHEMICAL PREPARATION; IODINE 125; LABELLED COMPOUNDS; MYOCARDIAL INFARCTION; SENSITIVITY; AMINO ACIDS; BETA DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; CARDIOVASCULAR DISEASES; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; RADIOASSAY; RADIOISOTOPES; REACTION KINETICS; SYNTHESIS; TRACER TECHNIQUES; TRANSFERASES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
6117117
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: CCATA
Submitting Site:
INIS
Size:
Pages: 153-163
Announcement Date:

Citation Formats

Willson, V J.C., Jones, H M, and Thompson, R J. Two-site immunoradiometric assay for the MB isoenzyme of creatine kinase. Netherlands: N. p., 1981. Web. doi:10.1016/0009-8981(81)90149-2.
Willson, V J.C., Jones, H M, & Thompson, R J. Two-site immunoradiometric assay for the MB isoenzyme of creatine kinase. Netherlands. doi:10.1016/0009-8981(81)90149-2.
Willson, V J.C., Jones, H M, and Thompson, R J. 1981. "Two-site immunoradiometric assay for the MB isoenzyme of creatine kinase." Netherlands. doi:10.1016/0009-8981(81)90149-2. https://www.osti.gov/servlets/purl/10.1016/0009-8981(81)90149-2.
@misc{etde_6117117,
title = {Two-site immunoradiometric assay for the MB isoenzyme of creatine kinase}
author = {Willson, V J.C., Jones, H M, and Thompson, R J}
abstractNote = {A two-site immunoradiometric assay for myocardial creatine kinase MB isoenzyme is described. The method utilizes immobilized anti-human creatine kinase BB antibodies and /sup 125/I-labelled anti-human creatine kinase MM antibodies and can specifically detect creatine kinase MB in the presence of approximately 1000-fold excess of creatine kinase MM or BB. Native kinase MB prepared from human heart and creatine kinase MB prepared by hybridisation of purified human creatine kinase MM and creatine kinase BB appeared to react identically in the assay. Serum estimations on patients with suspected myocardial infarction correlated with the presence of MB band on electrophoresis but preliminary results suggest that the two-site immunoradiometric assay may be more sensitive.}
doi = {10.1016/0009-8981(81)90149-2}
journal = {Clin. Chim. Acta; (Netherlands)}
volume = {113:2}
journal type = {AC}
place = {Netherlands}
year = {1981}
month = {Jun}
}