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Detection of cryptic species

Abstract

Morphologically similar cryptic species are common in insects. In Anopheles mosquitoes morphologically described species are complexes of cryptic species. Cryptic species are of great practical importance for two reasons: first, one or more species of the complex might not be a pest and control efforts directed at the complex as a whole would therefore be partly wasted; and second, genetic (and perhaps biological) control strategies directed against one species of the complex would not affect other species of the complex. At least one SIT effort has failed because the released sterile insect were of a different species and therefore did not mate with the wild insects being targeted. We use a multidisciplinary approach for detection of cryptic species complexes, focusing first on identifying variability in wild populations using RFLPs of mitochondrial and ribosomal RNA genes (mtDNA and rDNA); followed by confirmation using a variety of other techniques. For rapid identification of wild individuals of field collections, we use a DNA dot blot assay. DNA probes can be isolated by differential screening, however we are currently focusing on the sequencing of the rDNA extragenic spacers. These regions are repeated several hundred times per genome in mosquitoes and evolve rapidly. Molecular drive  More>>
Authors:
Cockburn, A F; Jensen, T; Seawright, J A [1] 
  1. United States Dept. of Agriculture, Agricultural Research Service, Medical and Veterinary Entomology Research Lab., Gainesville, FL (United States)
Publication Date:
Jan 01, 1998
Product Type:
Conference
Report Number:
IAEA-TECDOC-993; CONF-9411348-
Reference Number:
SCA: 553003; PA: AIX-29:029254; EDB-98:063233; SN: 98001965051
Resource Relation:
Conference: Research co-ordination meeting on genetic engineering technology for the improvement of the sterile insect technique, Vienna (Austria), 21-25 Nov 1994; Other Information: DN: 11 refs, 2 figs, 2 tabs.; PBD: Jan 1998; Related Information: Is Part Of Genetic engineering technology for the improvement of the sterile insect technique. Proceedings of a final research co-ordination meeting; PB: 78 p.
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; AGRICULTURE; DEVELOPING COUNTRIES; DNA SEQUENCERS; GENETIC ENGINEERING; GENETIC VARIABILITY; INSECTS; MORPHOLOGY; MOSQUITOES; PEST CONTROL; PROBES; STERILE MALE TECHNIQUE
OSTI ID:
610879
Research Organizations:
Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna (Austria).
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 1011-4289; Other: ON: DE98624509; TRN: XA9846584029254
Availability:
INIS; OSTI as DE98624509
Submitting Site:
INIS
Size:
pp. 31-36
Announcement Date:

Citation Formats

Cockburn, A F, Jensen, T, and Seawright, J A. Detection of cryptic species. IAEA: N. p., 1998. Web.
Cockburn, A F, Jensen, T, & Seawright, J A. Detection of cryptic species. IAEA.
Cockburn, A F, Jensen, T, and Seawright, J A. 1998. "Detection of cryptic species." IAEA.
@misc{etde_610879,
title = {Detection of cryptic species}
author = {Cockburn, A F, Jensen, T, and Seawright, J A}
abstractNote = {Morphologically similar cryptic species are common in insects. In Anopheles mosquitoes morphologically described species are complexes of cryptic species. Cryptic species are of great practical importance for two reasons: first, one or more species of the complex might not be a pest and control efforts directed at the complex as a whole would therefore be partly wasted; and second, genetic (and perhaps biological) control strategies directed against one species of the complex would not affect other species of the complex. At least one SIT effort has failed because the released sterile insect were of a different species and therefore did not mate with the wild insects being targeted. We use a multidisciplinary approach for detection of cryptic species complexes, focusing first on identifying variability in wild populations using RFLPs of mitochondrial and ribosomal RNA genes (mtDNA and rDNA); followed by confirmation using a variety of other techniques. For rapid identification of wild individuals of field collections, we use a DNA dot blot assay. DNA probes can be isolated by differential screening, however we are currently focusing on the sequencing of the rDNA extragenic spacers. These regions are repeated several hundred times per genome in mosquitoes and evolve rapidly. Molecular drive tends to keen the individual genes homogeneous within a species. (author). 11 refs, 2 figs, 2 tabs.}
place = {IAEA}
year = {1998}
month = {Jan}
}