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Studies on a novel peptide isolated and purified from rat insulinoma tissue

Thesis/Dissertation:

Abstract

Rat insulinoma peptide (RIP) which appears to be either a fragment of, or an altered rat C-peptide was isolated and purified by dialysis. The purity of this peptide was investigated using polyacrylamide gel electrophoresis with sodium dodecyl sulfate, isoelectric focusing, and high performance liquid chromatography. RIP may contain two peptides similar to each other but differing in their isoelectric points. The molecular weight of RIP was found to be 1982 daltons by fast atoms bombardment mass spectrometry giving a chain length of approximately 22 amino acid residues. From information obtained using radioimmunoassay employing antiserum R901, RIP appears to share a common C-terminus with rat C-peptide. A radioimmunoassay for RIP was developed using the purified RIP as immunogen and for standards and tracers. An indirect enzyme linked immunosorbent assay (ELISA) for rat insulinoma peptide was developed using purified RIP for immunogen and semi-purified RIP as a standard.
Authors:
Publication Date:
Jan 01, 1987
Product Type:
Thesis/Dissertation
Reference Number:
EDB-89-094219
Resource Relation:
Other Information: Thesis (Ph. D.). BRDX-81,893
Subject:
59 BASIC BIOLOGICAL SCIENCES; PEPTIDES; RADIOIMMUNOASSAY; ELECTROPHORESIS; ENZYME IMMUNOASSAY; LIQUID COLUMN CHROMATOGRAPHY; MASS SPECTROSCOPY; MOLECULAR WEIGHT; RATS; ANIMALS; CHROMATOGRAPHY; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; MAMMALS; ORGANIC COMPOUNDS; PROTEINS; RADIOASSAY; RADIOIMMUNOLOGY; RODENTS; SEPARATION PROCESSES; SPECTROSCOPY; TRACER TECHNIQUES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques
OSTI ID:
6101511
Research Organizations:
Surrey Univ., Guildford (UK)
Country of Origin:
United Kingdom
Language:
English
Availability:
UMI, Dissertation Information Service, 300 North Zeeb Road, Ann Arbor, MI 48106.
Submitting Site:
JMT
Size:
Pages: 224
Announcement Date:
May 13, 2001

Thesis/Dissertation:

Citation Formats

Al-Akhras, G N. Studies on a novel peptide isolated and purified from rat insulinoma tissue. United Kingdom: N. p., 1987. Web.
Al-Akhras, G N. Studies on a novel peptide isolated and purified from rat insulinoma tissue. United Kingdom.
Al-Akhras, G N. 1987. "Studies on a novel peptide isolated and purified from rat insulinoma tissue." United Kingdom.
@misc{etde_6101511,
title = {Studies on a novel peptide isolated and purified from rat insulinoma tissue}
author = {Al-Akhras, G N}
abstractNote = {Rat insulinoma peptide (RIP) which appears to be either a fragment of, or an altered rat C-peptide was isolated and purified by dialysis. The purity of this peptide was investigated using polyacrylamide gel electrophoresis with sodium dodecyl sulfate, isoelectric focusing, and high performance liquid chromatography. RIP may contain two peptides similar to each other but differing in their isoelectric points. The molecular weight of RIP was found to be 1982 daltons by fast atoms bombardment mass spectrometry giving a chain length of approximately 22 amino acid residues. From information obtained using radioimmunoassay employing antiserum R901, RIP appears to share a common C-terminus with rat C-peptide. A radioimmunoassay for RIP was developed using the purified RIP as immunogen and for standards and tracers. An indirect enzyme linked immunosorbent assay (ELISA) for rat insulinoma peptide was developed using purified RIP for immunogen and semi-purified RIP as a standard.}
place = {United Kingdom}
year = {1987}
month = {Jan}
}