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Immune adherence: a quantitative and kinetic analysis

Journal Article:

Abstract

Quantitative and kinetic analysis of the immune-adherence reaction (IA) between C3b fragments and IA receptors as an agglutination reaction is difficult. Analysis is possible, however, by use of radio-iodinated bovine serum albumin as antigen at low concentrations (less than 200 ng/ml) and optimal concentration of antibody to avoid precipitation of antigen-antibody complexes with human erythrocytes without participation of complement. Antigen and antibody are reacted at 37/sup 0/C, complement is added, the mixture incubated and human erythrocytes added; after further incubation, ice-cold EDTA containing buffer is added and the erythrocytes centrifuged and assayed for radioactivity. Control cells reacted with heated guinea pig serum retained less than 5% of the added radioactivity. The method facilitates measurement of IA reactivity and permits more detailed analysis of the mechanism underlying the reaction.
Authors:
Sekine, T [1] 
  1. National Cancer Center, Tokyo (Japan). Research Inst.
Publication Date:
Sep 01, 1978
Product Type:
Journal Article
Reference Number:
AIX-10-430141; EDB-79-113258
Resource Relation:
Journal Name: J. Immunol. Methods; (Netherlands); Journal Volume: 23:1
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANTIGEN-ANTIBODY REACTIONS; TRACER TECHNIQUES; ALBUMINS; ANTIBODIES; ANTIGENS; BIOCHEMICAL REACTION KINETICS; BLOOD SERUM; CATTLE; ERYTHROCYTES; GUINEA PIGS; IMMUNITY; INCUBATION; IODINE 125; LABELLED COMPOUNDS; MAN; MEDIUM TEMPERATURE; QUANTITATIVE CHEMICAL ANALYSIS; RADIOASSAY; RECEPTORS; ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CHEMICAL ANALYSIS; DAYS LIVING RADIOISOTOPES; DOMESTIC ANIMALS; ELECTRON CAPTURE RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PRIMATES; PROTEINS; RADIOISOTOPES; REACTION KINETICS; RODENTS; RUMINANTS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques
OSTI ID:
6101485
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JIMMB
Submitting Site:
INIS
Size:
Pages: 99-108
Announcement Date:
May 01, 1979

Journal Article:

Citation Formats

Sekine, T. Immune adherence: a quantitative and kinetic analysis. Netherlands: N. p., 1978. Web.
Sekine, T. Immune adherence: a quantitative and kinetic analysis. Netherlands.
Sekine, T. 1978. "Immune adherence: a quantitative and kinetic analysis." Netherlands.
@misc{etde_6101485,
title = {Immune adherence: a quantitative and kinetic analysis}
author = {Sekine, T}
abstractNote = {Quantitative and kinetic analysis of the immune-adherence reaction (IA) between C3b fragments and IA receptors as an agglutination reaction is difficult. Analysis is possible, however, by use of radio-iodinated bovine serum albumin as antigen at low concentrations (less than 200 ng/ml) and optimal concentration of antibody to avoid precipitation of antigen-antibody complexes with human erythrocytes without participation of complement. Antigen and antibody are reacted at 37/sup 0/C, complement is added, the mixture incubated and human erythrocytes added; after further incubation, ice-cold EDTA containing buffer is added and the erythrocytes centrifuged and assayed for radioactivity. Control cells reacted with heated guinea pig serum retained less than 5% of the added radioactivity. The method facilitates measurement of IA reactivity and permits more detailed analysis of the mechanism underlying the reaction.}
journal = {J. Immunol. Methods; (Netherlands)}
volume = {23:1}
journal type = {AC}
place = {Netherlands}
year = {1978}
month = {Sep}
}