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Functional role of zinc in poly(A) synthesis catalyzed by nuclear poly(A) polymerase

Journal Article:

Abstract

The functional role of transition metals in poly(A) synthesis was elucidated by investigating the effect of the metal chelator o-Phenanthroline on purified nuclear poly(A) polymerase. This chelator inhibited the enzyme activity in a manner competitive with respect to the polynucleotide primer concentration. o-phenanthroline was a non-competitive inhibitor with regard to ATP concentration and an 'uncompetitive' inhibitor with regard to dithiothreitor levels. The metal content of the purified enzyme preparations from rat liver and Morris hepatoma 3924A was determined using atomic absorption spectrometry. Of the transition metals measured, only zinc was present in detectable quantities at levels less than 1 mol/mol of enzyme. Hepatoma enzyme contained 2-3 times as much zinc as the corresponding liver enzyme. Hepatoma poly(A) polymerase was also radioactively labelled in vivo by injection of tumor-bearing animals with /sup 65/Zn. Dialysis experiments with highly purified radiolabelled poly(A) polymerase showed that the enzyme-zinc complex was labile and that a reduction in /sup 65/Zn content correlated with a loss in enzyme activity.
Publication Date:
Jul 01, 1978
Product Type:
Journal Article
Reference Number:
AIX-10-420982; EDB-79-113265
Resource Relation:
Journal Name: Eur. J. Biochem.; (Germany, Federal Republic of); Journal Volume: 88:1
Subject:
59 BASIC BIOLOGICAL SCIENCES; CHELATES; BIOLOGICAL EFFECTS; ENZYME ACTIVITY; INHIBITION; NUCLEOTIDES; BIOSYNTHESIS; POLYMERASES; ZINC; ATP; HEPATOMAS; IN VIVO; LIVER; RATS; TRACER TECHNIQUES; TUMOR CELLS; ZINC 65; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; BODY; COMPLEXES; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; ELEMENTS; ENZYMES; EVEN-ODD NUCLEI; GLANDS; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; METALS; NEOPLASMS; NUCLEI; ORGANIC COMPOUNDS; ORGANS; RADIOISOTOPES; RODENTS; SYNTHESIS; VERTEBRATES; ZINC ISOTOPES; 550201* - Biochemistry- Tracer Techniques; 551001 - Physiological Systems- Tracer Techniques
OSTI ID:
6101432
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: EJBCA
Submitting Site:
INIS
Size:
Pages: 29-36
Announcement Date:
Feb 01, 1979

Journal Article:

Citation Formats

Rose, K M, Allen, M S, Crawford, I L, Jacob, S T, Pennsylvania State Univ., Hershey (USA). Specialized Cancer Research Center, Texas Univ., Dallas (USA). Dept. of Neurology, and Texas Univ., Dallas (USA). Dept. of Pharmacology). Functional role of zinc in poly(A) synthesis catalyzed by nuclear poly(A) polymerase. Germany: N. p., 1978. Web.
Rose, K M, Allen, M S, Crawford, I L, Jacob, S T, Pennsylvania State Univ., Hershey (USA). Specialized Cancer Research Center, Texas Univ., Dallas (USA). Dept. of Neurology, & Texas Univ., Dallas (USA). Dept. of Pharmacology). Functional role of zinc in poly(A) synthesis catalyzed by nuclear poly(A) polymerase. Germany.
Rose, K M, Allen, M S, Crawford, I L, Jacob, S T, Pennsylvania State Univ., Hershey (USA). Specialized Cancer Research Center, Texas Univ., Dallas (USA). Dept. of Neurology, and Texas Univ., Dallas (USA). Dept. of Pharmacology). 1978. "Functional role of zinc in poly(A) synthesis catalyzed by nuclear poly(A) polymerase." Germany.
@misc{etde_6101432,
title = {Functional role of zinc in poly(A) synthesis catalyzed by nuclear poly(A) polymerase}
author = {Rose, K M, Allen, M S, Crawford, I L, Jacob, S T, Pennsylvania State Univ., Hershey (USA). Specialized Cancer Research Center, Texas Univ., Dallas (USA). Dept. of Neurology, and Texas Univ., Dallas (USA). Dept. of Pharmacology)}
abstractNote = {The functional role of transition metals in poly(A) synthesis was elucidated by investigating the effect of the metal chelator o-Phenanthroline on purified nuclear poly(A) polymerase. This chelator inhibited the enzyme activity in a manner competitive with respect to the polynucleotide primer concentration. o-phenanthroline was a non-competitive inhibitor with regard to ATP concentration and an 'uncompetitive' inhibitor with regard to dithiothreitor levels. The metal content of the purified enzyme preparations from rat liver and Morris hepatoma 3924A was determined using atomic absorption spectrometry. Of the transition metals measured, only zinc was present in detectable quantities at levels less than 1 mol/mol of enzyme. Hepatoma enzyme contained 2-3 times as much zinc as the corresponding liver enzyme. Hepatoma poly(A) polymerase was also radioactively labelled in vivo by injection of tumor-bearing animals with /sup 65/Zn. Dialysis experiments with highly purified radiolabelled poly(A) polymerase showed that the enzyme-zinc complex was labile and that a reduction in /sup 65/Zn content correlated with a loss in enzyme activity.}
journal = {Eur. J. Biochem.; (Germany, Federal Republic of)}
volume = {88:1}
journal type = {AC}
place = {Germany}
year = {1978}
month = {Jul}
}