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Labelling of glycerolipids in the cotyledons of developing oilseeds by (1-/sup 14/C) acetate and (2-/sup 3/H) glycerol

Journal Article:

Abstract

3-sn-Phosphatidylcholine was identified as the major lipid in cotyledons from the developing seeds of soya bean, linseed and safflower when tissue was steamed before lipid extraction. Incubation for up to 3h changed the composition of this lipid and of diacylglycerol. Labelling studies, including pulse-chase experiments, were carried out with (1-/sup 14/C) acetate and (2-/sup 3/H) glycerol supplied to detached cotyledons. The results suggest a rapid turnover of 3-sn-phosphatidylcholine during triacylglycerol accumulation in developing oilseeds, and are consistent with the operation of a biosynthetic route whereby oleate initially esterified to the phospholipid is first desaturated, then polyunsaturated fatty acids transferred to triacylglycerol, via diacylglycerol. The possible role of oleoyl phosphatidylcholine as a substrate for oleate desaturation is discussed.
Authors:
Slack, C R; Roughan, P G; Balasingham, N [1] 
  1. Department of Scientific and Industrial Research, Palmerston North (New Zealand). Div. of Plant Physiology
Publication Date:
Feb 15, 1978
Product Type:
Journal Article
Reference Number:
AIX-10-421015; EDB-79-113280
Resource Relation:
Journal Name: Biochem. J.; (United Kingdom); Journal Volume: 170:2
Subject:
59 BASIC BIOLOGICAL SCIENCES; LIPIDS; BIOSYNTHESIS; SEEDS; ACETATES; BIOCHEMICAL REACTION KINETICS; CARBON 14 COMPOUNDS; CHOLINE; COMPARATIVE EVALUATIONS; FLAX PLANTS; GERMINATION; GLYCEROL; GLYCINE HISPIDA; LABELLING; METABOLISM; OLEIC ACID; PHOSPHOLIPIDS; TRACER TECHNIQUES; TRIGLYCERIDES; TRITIUM COMPOUNDS; ALCOHOLS; AMINES; AMMONIUM COMPOUNDS; CARBOXYLIC ACID SALTS; CARBOXYLIC ACIDS; ESTERS; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; KINETICS; LABELLED COMPOUNDS; LEGUMINOSAE; LIPOTROPIC FACTORS; MONOCARBOXYLIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; PLANTS; QUATERNARY COMPOUNDS; REACTION KINETICS; SYNTHESIS; 550201* - Biochemistry- Tracer Techniques
OSTI ID:
6101352
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BIJOA
Submitting Site:
INIS
Size:
Pages: 421-433
Announcement Date:

Journal Article:

Citation Formats

Slack, C R, Roughan, P G, and Balasingham, N. Labelling of glycerolipids in the cotyledons of developing oilseeds by (1-/sup 14/C) acetate and (2-/sup 3/H) glycerol. United Kingdom: N. p., 1978. Web.
Slack, C R, Roughan, P G, & Balasingham, N. Labelling of glycerolipids in the cotyledons of developing oilseeds by (1-/sup 14/C) acetate and (2-/sup 3/H) glycerol. United Kingdom.
Slack, C R, Roughan, P G, and Balasingham, N. 1978. "Labelling of glycerolipids in the cotyledons of developing oilseeds by (1-/sup 14/C) acetate and (2-/sup 3/H) glycerol." United Kingdom.
@misc{etde_6101352,
title = {Labelling of glycerolipids in the cotyledons of developing oilseeds by (1-/sup 14/C) acetate and (2-/sup 3/H) glycerol}
author = {Slack, C R, Roughan, P G, and Balasingham, N}
abstractNote = {3-sn-Phosphatidylcholine was identified as the major lipid in cotyledons from the developing seeds of soya bean, linseed and safflower when tissue was steamed before lipid extraction. Incubation for up to 3h changed the composition of this lipid and of diacylglycerol. Labelling studies, including pulse-chase experiments, were carried out with (1-/sup 14/C) acetate and (2-/sup 3/H) glycerol supplied to detached cotyledons. The results suggest a rapid turnover of 3-sn-phosphatidylcholine during triacylglycerol accumulation in developing oilseeds, and are consistent with the operation of a biosynthetic route whereby oleate initially esterified to the phospholipid is first desaturated, then polyunsaturated fatty acids transferred to triacylglycerol, via diacylglycerol. The possible role of oleoyl phosphatidylcholine as a substrate for oleate desaturation is discussed.}
journal = {Biochem. J.; (United Kingdom)}
volume = {170:2}
journal type = {AC}
place = {United Kingdom}
year = {1978}
month = {Feb}
}