Abstract
A solid state fermentation process was developed for the conversion of straw and cellulose under anaerobic conditions by a mixed culture of cellulolytic and methanogenic organisms. The bioconversion rate and efficiency were compared under mesophilic (35/sup 0/C) and thermophilic (55/sup 0/C) conditions. Cellulolytic activity was assayed in terms of sugar and overall soluble organic matter (chemical oxygen demand, COD) production. Maximum conversion rates were obtained under thermophilic conditions, i.e. 8.4 g and 14.2 g COD/kg.d, respectively, when wheat straw and cellulose were used as substrates. The cellulolytic activity of the reactor contents (23% dry matter), measured under substrate excess conditions, amounted to 50 g COD/kg.d. As a comparison, the activity of rumen contents (15 % dry matter) measured by the same assay amounted to 150 g COD/kg . d. The anaerobic cellulases appeared to be substrate bound. This and the relative low activity levels attained, limit the perspectives of producing cellulase enzymes by this type of process.
Citation Formats
Vandevoorde, L, and Verstraete, W.
Anaerobic solid state fermentation of cellulosic substrates with possible application to cellulase production.
Germany: N. p.,
1987.
Web.
Vandevoorde, L, & Verstraete, W.
Anaerobic solid state fermentation of cellulosic substrates with possible application to cellulase production.
Germany.
Vandevoorde, L, and Verstraete, W.
1987.
"Anaerobic solid state fermentation of cellulosic substrates with possible application to cellulase production."
Germany.
@misc{etde_6101327,
title = {Anaerobic solid state fermentation of cellulosic substrates with possible application to cellulase production}
author = {Vandevoorde, L, and Verstraete, W}
abstractNote = {A solid state fermentation process was developed for the conversion of straw and cellulose under anaerobic conditions by a mixed culture of cellulolytic and methanogenic organisms. The bioconversion rate and efficiency were compared under mesophilic (35/sup 0/C) and thermophilic (55/sup 0/C) conditions. Cellulolytic activity was assayed in terms of sugar and overall soluble organic matter (chemical oxygen demand, COD) production. Maximum conversion rates were obtained under thermophilic conditions, i.e. 8.4 g and 14.2 g COD/kg.d, respectively, when wheat straw and cellulose were used as substrates. The cellulolytic activity of the reactor contents (23% dry matter), measured under substrate excess conditions, amounted to 50 g COD/kg.d. As a comparison, the activity of rumen contents (15 % dry matter) measured by the same assay amounted to 150 g COD/kg . d. The anaerobic cellulases appeared to be substrate bound. This and the relative low activity levels attained, limit the perspectives of producing cellulase enzymes by this type of process.}
journal = []
volume = {26:5}
journal type = {AC}
place = {Germany}
year = {1987}
month = {Aug}
}
title = {Anaerobic solid state fermentation of cellulosic substrates with possible application to cellulase production}
author = {Vandevoorde, L, and Verstraete, W}
abstractNote = {A solid state fermentation process was developed for the conversion of straw and cellulose under anaerobic conditions by a mixed culture of cellulolytic and methanogenic organisms. The bioconversion rate and efficiency were compared under mesophilic (35/sup 0/C) and thermophilic (55/sup 0/C) conditions. Cellulolytic activity was assayed in terms of sugar and overall soluble organic matter (chemical oxygen demand, COD) production. Maximum conversion rates were obtained under thermophilic conditions, i.e. 8.4 g and 14.2 g COD/kg.d, respectively, when wheat straw and cellulose were used as substrates. The cellulolytic activity of the reactor contents (23% dry matter), measured under substrate excess conditions, amounted to 50 g COD/kg.d. As a comparison, the activity of rumen contents (15 % dry matter) measured by the same assay amounted to 150 g COD/kg . d. The anaerobic cellulases appeared to be substrate bound. This and the relative low activity levels attained, limit the perspectives of producing cellulase enzymes by this type of process.}
journal = []
volume = {26:5}
journal type = {AC}
place = {Germany}
year = {1987}
month = {Aug}
}