Abstract
The authors have isolated a series of 14 spontaneously arising and 28 X-ray-induced mutants at the hypoxanthine-guanine phosphoribosyltransferase (hgprt) locus in human lymphoblastoid cells. Among the spontaneous mutants, 5/14 (36%) had detectable alterations in their restriction fragment pattern after hybridization with a human cDNA probe for hgprt. Of the 10 remaining mutants, 4 had partial HGPRT enzyme activity, which suggested that they contained point mutations. Among the 28 mutants induced by 150 rad of X-rays, 15 (54%) had deletions of part or all of the hgprt gene. Of the remaining 13 (18% overall) 5 had partial HGPRT enzyme activity, which suggested that they contained point mutations. These data imply that in this human cell system, X-rays induce both point mutants which have residual enzyme activity as well as mutations involving relatively large deletions of DNA. 48 reference, 1 figure, 4 tables.
Citation Formats
Liber, H L, Call, K M, and Little, J B.
Molecular and biochemical analyses of spontaneous and X-ray-induced mutants in human lymphoblastoid cells.
Netherlands: N. p.,
1987.
Web.
Liber, H L, Call, K M, & Little, J B.
Molecular and biochemical analyses of spontaneous and X-ray-induced mutants in human lymphoblastoid cells.
Netherlands.
Liber, H L, Call, K M, and Little, J B.
1987.
"Molecular and biochemical analyses of spontaneous and X-ray-induced mutants in human lymphoblastoid cells."
Netherlands.
@misc{etde_6064695,
title = {Molecular and biochemical analyses of spontaneous and X-ray-induced mutants in human lymphoblastoid cells}
author = {Liber, H L, Call, K M, and Little, J B}
abstractNote = {The authors have isolated a series of 14 spontaneously arising and 28 X-ray-induced mutants at the hypoxanthine-guanine phosphoribosyltransferase (hgprt) locus in human lymphoblastoid cells. Among the spontaneous mutants, 5/14 (36%) had detectable alterations in their restriction fragment pattern after hybridization with a human cDNA probe for hgprt. Of the 10 remaining mutants, 4 had partial HGPRT enzyme activity, which suggested that they contained point mutations. Among the 28 mutants induced by 150 rad of X-rays, 15 (54%) had deletions of part or all of the hgprt gene. Of the remaining 13 (18% overall) 5 had partial HGPRT enzyme activity, which suggested that they contained point mutations. These data imply that in this human cell system, X-rays induce both point mutants which have residual enzyme activity as well as mutations involving relatively large deletions of DNA. 48 reference, 1 figure, 4 tables.}
journal = []
volume = {178:1}
journal type = {AC}
place = {Netherlands}
year = {1987}
month = {May}
}
title = {Molecular and biochemical analyses of spontaneous and X-ray-induced mutants in human lymphoblastoid cells}
author = {Liber, H L, Call, K M, and Little, J B}
abstractNote = {The authors have isolated a series of 14 spontaneously arising and 28 X-ray-induced mutants at the hypoxanthine-guanine phosphoribosyltransferase (hgprt) locus in human lymphoblastoid cells. Among the spontaneous mutants, 5/14 (36%) had detectable alterations in their restriction fragment pattern after hybridization with a human cDNA probe for hgprt. Of the 10 remaining mutants, 4 had partial HGPRT enzyme activity, which suggested that they contained point mutations. Among the 28 mutants induced by 150 rad of X-rays, 15 (54%) had deletions of part or all of the hgprt gene. Of the remaining 13 (18% overall) 5 had partial HGPRT enzyme activity, which suggested that they contained point mutations. These data imply that in this human cell system, X-rays induce both point mutants which have residual enzyme activity as well as mutations involving relatively large deletions of DNA. 48 reference, 1 figure, 4 tables.}
journal = []
volume = {178:1}
journal type = {AC}
place = {Netherlands}
year = {1987}
month = {May}
}