You need JavaScript to view this

Quantitative immunoelectrophoretic analysis of extract from cow hair and dander

Abstract

Quantitative immunoelectrophoresis used for the analysis of a dialysed, centrifuged and freeze-dried extract from cow hair and dander revealed 17 antigens. Five of these were identified as serum proteins. Partial identity to antigens of serum and extract from hair and dander of goat, sheep, swine, horse, dog, cat, and guinea pig, and to antigens of house dust was demonstrated. Sera from 36 patients with manifest allergy to cow hair and dander selected on the basis of case history, RAST, skin and provocation test, were examined in crossed radioimmunoelectrophoresis (CRIE); sera from five persons with high serum IgE, but without allergy to cow hair and dander, and sera from five normal individuals were controls. 31/36 of the sera contained IgE with specific affinity for two of the antigens of the extract. Further, two major and six minor allergens were identified. The control sera showed no specific IgE binding. A significant positive correlation was found between RAST and CRIE for the first group of patients. The approximate molecular weights of the four major allergens obtained by means of gel chromatography were: 2.4 x 10/sup 4/, 2 x 10/sup 4/, 2 x 10/sup 5/ dalton, respectively. Using Con-A and Con-A Sepharose in crossed  More>>
Authors:
Prahl, P; Weeke, B; Loewenstein, H [1] 
  1. Rigshospitalet, Copenhagen (Denmark)
Publication Date:
Jan 01, 1978
Product Type:
Journal Article
Reference Number:
AIX-10-441293; EDB-79-113289
Resource Relation:
Journal Name: Allergy (Copenhagen); (Denmark); Journal Volume: 33
Subject:
59 BASIC BIOLOGICAL SCIENCES; ALLERGY; RADIOIMMUNOASSAY; ANTIGENS; COWS; ELECTROPHORESIS; HAIR; PATIENTS; QUANTITATIVE CHEMICAL ANALYSIS; ANIMALS; BODY; CATTLE; CHEMICAL ANALYSIS; DISEASES; DOMESTIC ANIMALS; ISOTOPE APPLICATIONS; MAMMALS; ORGANS; PATHOLOGICAL CHANGES; RADIOASSAY; RUMINANTS; SKIN; TRACER TECHNIQUES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques
OSTI ID:
6057508
Country of Origin:
Denmark
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: LLRGD
Submitting Site:
INIS
Size:
Pages: 241-253
Announcement Date:
May 01, 1979

Citation Formats

Prahl, P, Weeke, B, and Loewenstein, H. Quantitative immunoelectrophoretic analysis of extract from cow hair and dander. Denmark: N. p., 1978. Web. doi:10.1111/j.1398-9995.1978.tb01544.x.
Prahl, P, Weeke, B, & Loewenstein, H. Quantitative immunoelectrophoretic analysis of extract from cow hair and dander. Denmark. doi:10.1111/j.1398-9995.1978.tb01544.x.
Prahl, P, Weeke, B, and Loewenstein, H. 1978. "Quantitative immunoelectrophoretic analysis of extract from cow hair and dander." Denmark. doi:10.1111/j.1398-9995.1978.tb01544.x. https://www.osti.gov/servlets/purl/10.1111/j.1398-9995.1978.tb01544.x.
@misc{etde_6057508,
title = {Quantitative immunoelectrophoretic analysis of extract from cow hair and dander}
author = {Prahl, P, Weeke, B, and Loewenstein, H}
abstractNote = {Quantitative immunoelectrophoresis used for the analysis of a dialysed, centrifuged and freeze-dried extract from cow hair and dander revealed 17 antigens. Five of these were identified as serum proteins. Partial identity to antigens of serum and extract from hair and dander of goat, sheep, swine, horse, dog, cat, and guinea pig, and to antigens of house dust was demonstrated. Sera from 36 patients with manifest allergy to cow hair and dander selected on the basis of case history, RAST, skin and provocation test, were examined in crossed radioimmunoelectrophoresis (CRIE); sera from five persons with high serum IgE, but without allergy to cow hair and dander, and sera from five normal individuals were controls. 31/36 of the sera contained IgE with specific affinity for two of the antigens of the extract. Further, two major and six minor allergens were identified. The control sera showed no specific IgE binding. A significant positive correlation was found between RAST and CRIE for the first group of patients. The approximate molecular weights of the four major allergens obtained by means of gel chromatography were: 2.4 x 10/sup 4/, 2 x 10/sup 4/, 2 x 10/sup 5/ dalton, respectively. Using Con-A and Con-A Sepharose in crossed immunoaffinoelectrophoresis, eight of the antigens were revealed to contain groups with affinity for Con-A.}
doi = {10.1111/j.1398-9995.1978.tb01544.x}
journal = {Allergy (Copenhagen); (Denmark)}
volume = {33}
journal type = {AC}
place = {Denmark}
year = {1978}
month = {Jan}
}