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Antibodies to lactalbumin interfere with its radioimmunoassay in human plasma

Abstract

Two radioimmunoassays for human lactalbumin have been established using a rabbit antiserum. One assay uses a second antibody to separate bound from free label; the other uses polyethylene glycol to precipitate gamma globulin non-specifically. It is confirmed that about half the normal human population have a substance in their blood which inhibits the binding of lactalbumin to the rabbit antibody. Comparison of the two assays has demonstrated that this material is not lactalbumin but a naturally occurring antibody. It is shown that it is in the IgG fraction of human plasma and is probably a cross-reacting antibody to bovine lactalbumin. None out of fifteen males and fourteen out of fifty eight non-pregnant, non-lacatating females had low levels of lactalbumin in their blood (0.6-2.0 ng/ml). The assay could not detect a statistically significant difference between normal women and those with either benign breast disease or metastatic mammary carcinoma.
Authors:
Stevens, U; Laurence, D J.R.; [1]  Ormerod, M G [2] 
  1. Royal Marsden Hospital, London (UK)
  2. Institute of Cancer Research, Sutton (UK). Surrey Branch
Publication Date:
Jan 01, 1978
Product Type:
Journal Article
Reference Number:
AIX-10-421064; EDB-79-113415
Resource Relation:
Journal Name: Clin. Chim. Acta; (Netherlands); Journal Volume: 87:1
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; ALBUMINS; RADIOIMMUNOASSAY; BLOOD PLASMA; MAMMARY GLANDS; NEOPLASMS; DIAGNOSTIC TECHNIQUES; ANTIBODIES; GLOBULINS-GAMMA; IMMUNE SERUMS; MEN; POLYETHYLENE GLYCOLS; RABBITS; WOMEN; ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BODY; BODY FLUIDS; DISEASES; FEMALES; GLANDS; GLOBULINS; ISOTOPE APPLICATIONS; MALES; MAMMALS; MAN; ORGANIC COMPOUNDS; ORGANIC POLYMERS; ORGANS; POLYMERS; PRIMATES; PROTEINS; RADIOASSAY; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics; 551001 - Physiological Systems- Tracer Techniques
OSTI ID:
6056521
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: CCATA
Submitting Site:
INIS
Size:
Pages: 149-157
Announcement Date:

Citation Formats

Stevens, U, Laurence, D J.R., and Ormerod, M G. Antibodies to lactalbumin interfere with its radioimmunoassay in human plasma. Netherlands: N. p., 1978. Web. doi:10.1016/0009-8981(78)90069-4.
Stevens, U, Laurence, D J.R., & Ormerod, M G. Antibodies to lactalbumin interfere with its radioimmunoassay in human plasma. Netherlands. doi:10.1016/0009-8981(78)90069-4.
Stevens, U, Laurence, D J.R., and Ormerod, M G. 1978. "Antibodies to lactalbumin interfere with its radioimmunoassay in human plasma." Netherlands. doi:10.1016/0009-8981(78)90069-4. https://www.osti.gov/servlets/purl/10.1016/0009-8981(78)90069-4.
@misc{etde_6056521,
title = {Antibodies to lactalbumin interfere with its radioimmunoassay in human plasma}
author = {Stevens, U, Laurence, D J.R., and Ormerod, M G}
abstractNote = {Two radioimmunoassays for human lactalbumin have been established using a rabbit antiserum. One assay uses a second antibody to separate bound from free label; the other uses polyethylene glycol to precipitate gamma globulin non-specifically. It is confirmed that about half the normal human population have a substance in their blood which inhibits the binding of lactalbumin to the rabbit antibody. Comparison of the two assays has demonstrated that this material is not lactalbumin but a naturally occurring antibody. It is shown that it is in the IgG fraction of human plasma and is probably a cross-reacting antibody to bovine lactalbumin. None out of fifteen males and fourteen out of fifty eight non-pregnant, non-lacatating females had low levels of lactalbumin in their blood (0.6-2.0 ng/ml). The assay could not detect a statistically significant difference between normal women and those with either benign breast disease or metastatic mammary carcinoma.}
doi = {10.1016/0009-8981(78)90069-4}
journal = {Clin. Chim. Acta; (Netherlands)}
volume = {87:1}
journal type = {AC}
place = {Netherlands}
year = {1978}
month = {Jan}
}