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Effect of radiation on the expression of tumor-associated antigens of human lung adenocarcinoma cells. Immunological study using monoclonal antibodies

Journal Article:

Abstract

We studied the effects of irradiation on the expression of a tumor-associated antigen (YH206 antigen) of cultured human lung adenocarcinoma A549 cells by using enzyme-linked immunosorbent assay (ELISA) and flow cytometry. YH206 antigen is preferentially expressed on adenocarcinoma cells. Irradiation of A549 cells remarkably increased the expression of YH206 antigen on the cell surface and the level of the antigen in the culture supernatant as well as in the cell lysate, whereas it significantly affected the expression of HLA (MHC-class I) antigen on the same cells. The expression of HLA antigen on the cell was also increased after treatment of the cells with interferon-..gamma... In an additional experiment, cells were stained simultaneously for surface antigens (fluorescein coupled antibodies) and for DNA content (propidium iodide), and then dual parameter measurements were performed by flow cytometry to analyse the relationship between antigen levels and the cell cycle. YH206 antigen and HLA antigen increased more in the S and G/sub 2//M phases of the cell cycle than in G/sub 0//G/sub 1/. The expression of YH206 antigen was enhanced in the S and G/sub 2//M phases by irradiation, whereas the expression of HLA antigen was enhanced in each phase of the cell cycle with  More>>
Authors:
Publication Date:
Dec 01, 1988
Product Type:
Journal Article
Reference Number:
JPN-89-008249; EDB-89-087702
Resource Relation:
Journal Name: Nippon Igaku Hoshasen Gakkai Zasshi; (Japan); Journal Volume: 48:12
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ANTIGENS; BIOLOGICAL RADIATION EFFECTS; TUMOR CELLS; CARCINOMAS; CELL CULTURES; CELL CYCLE; CELL FLOW SYSTEMS; ENZYME IMMUNOASSAY; GROWTH; IMMUNITY; INTERFERON; LUNGS; MONOCLONAL ANTIBODIES; X RADIATION; ANIMAL CELLS; ANTIBODIES; BIOLOGICAL EFFECTS; BODY; DISEASES; ELECTROMAGNETIC RADIATION; GROWTH FACTORS; IMMUNOASSAY; IONIZING RADIATIONS; LYMPHOKINES; MITOGENS; NEOPLASMS; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIATION EFFECTS; RADIATIONS; RESPIRATORY SYSTEM; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture
OSTI ID:
6045838
Research Organizations:
Sapporo Medical Coll. (Japan)
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: CODEN: NHGZA
Submitting Site:
JPN
Size:
Pages: 1553-1562
Announcement Date:
Apr 01, 1989

Journal Article:

Citation Formats

Hareyama, Masato. Effect of radiation on the expression of tumor-associated antigens of human lung adenocarcinoma cells. Immunological study using monoclonal antibodies. Japan: N. p., 1988. Web.
Hareyama, Masato. Effect of radiation on the expression of tumor-associated antigens of human lung adenocarcinoma cells. Immunological study using monoclonal antibodies. Japan.
Hareyama, Masato. 1988. "Effect of radiation on the expression of tumor-associated antigens of human lung adenocarcinoma cells. Immunological study using monoclonal antibodies." Japan.
@misc{etde_6045838,
title = {Effect of radiation on the expression of tumor-associated antigens of human lung adenocarcinoma cells. Immunological study using monoclonal antibodies}
author = {Hareyama, Masato}
abstractNote = {We studied the effects of irradiation on the expression of a tumor-associated antigen (YH206 antigen) of cultured human lung adenocarcinoma A549 cells by using enzyme-linked immunosorbent assay (ELISA) and flow cytometry. YH206 antigen is preferentially expressed on adenocarcinoma cells. Irradiation of A549 cells remarkably increased the expression of YH206 antigen on the cell surface and the level of the antigen in the culture supernatant as well as in the cell lysate, whereas it significantly affected the expression of HLA (MHC-class I) antigen on the same cells. The expression of HLA antigen on the cell was also increased after treatment of the cells with interferon-..gamma... In an additional experiment, cells were stained simultaneously for surface antigens (fluorescein coupled antibodies) and for DNA content (propidium iodide), and then dual parameter measurements were performed by flow cytometry to analyse the relationship between antigen levels and the cell cycle. YH206 antigen and HLA antigen increased more in the S and G/sub 2//M phases of the cell cycle than in G/sub 0//G/sub 1/. The expression of YH206 antigen was enhanced in the S and G/sub 2//M phases by irradiation, whereas the expression of HLA antigen was enhanced in each phase of the cell cycle with irradiation or IFN. These results suggest that irradiation plays a key role in the change of the expression of certain tumor-associated antigens.}
journal = {Nippon Igaku Hoshasen Gakkai Zasshi; (Japan)}
volume = {48:12}
journal type = {AC}
place = {Japan}
year = {1988}
month = {Dec}
}