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Studies by radioiodination of normal adult, fetal and leukemic cell membranes

Journal Article:

Abstract

A comparison was made between cord blood lymphocytes, normal adult lymphocytes and leukemic cells after membrane iodination with lactoperoxidase. A double-labeling technique using lactoperoxidase iodination with /sup 125/I and /sup 131/I followed by analysis on polyacrylamide gel electrophoresis revealed a number of membrane differences between leukemic, normal and fetal cells. There was a reduction in the 70,000 molecular weight component in cord blood cells compared to adult lymphocytes, and an increase in membrane peptides with molecular weights of 35,000, 20,000, 9,000 and 4,000. Although smaller molecular weight peptides were also present in chronic lymphatic leukemia as well as acute myeloid leukemia, these were shown to be distinct from fetal type membrane components.
Authors:
Kannourakis, G; Cauchi, M N [1] 
  1. Department of Pathology and Immunology, Monash Medical School, Melbourne, Australia
Publication Date:
Jan 01, 1978
Product Type:
Journal Article
Reference Number:
AIX-10-458669; EDB-79-132860
Resource Relation:
Journal Name: Exp. Hematol.; (Denmark); Journal Volume: 6
Subject:
59 BASIC BIOLOGICAL SCIENCES; CELL MEMBRANES; COMPARATIVE EVALUATIONS; DOUBLE LABELLING; FETUSES; IODINATION; IODINE 125; IODINE 131; LEUKEMIA; MAN; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CELL CONSTITUENTS; CHEMICAL REACTIONS; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; HALOGENATION; HEMIC DISEASES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; LABELLING; MAMMALS; MEMBRANES; NEOPLASMS; NUCLEI; ODD-EVEN NUCLEI; PRIMATES; RADIOISOTOPES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques; 550901 - Pathology- Tracer Techniques
OSTI ID:
6045424
Country of Origin:
Denmark
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: EXHEB
Submitting Site:
INIS
Size:
Pages: 655-660
Announcement Date:

Journal Article:

Citation Formats

Kannourakis, G, and Cauchi, M N. Studies by radioiodination of normal adult, fetal and leukemic cell membranes. Denmark: N. p., 1978. Web.
Kannourakis, G, & Cauchi, M N. Studies by radioiodination of normal adult, fetal and leukemic cell membranes. Denmark.
Kannourakis, G, and Cauchi, M N. 1978. "Studies by radioiodination of normal adult, fetal and leukemic cell membranes." Denmark.
@misc{etde_6045424,
title = {Studies by radioiodination of normal adult, fetal and leukemic cell membranes}
author = {Kannourakis, G, and Cauchi, M N}
abstractNote = {A comparison was made between cord blood lymphocytes, normal adult lymphocytes and leukemic cells after membrane iodination with lactoperoxidase. A double-labeling technique using lactoperoxidase iodination with /sup 125/I and /sup 131/I followed by analysis on polyacrylamide gel electrophoresis revealed a number of membrane differences between leukemic, normal and fetal cells. There was a reduction in the 70,000 molecular weight component in cord blood cells compared to adult lymphocytes, and an increase in membrane peptides with molecular weights of 35,000, 20,000, 9,000 and 4,000. Although smaller molecular weight peptides were also present in chronic lymphatic leukemia as well as acute myeloid leukemia, these were shown to be distinct from fetal type membrane components.}
journal = {Exp. Hematol.; (Denmark)}
volume = {6}
journal type = {AC}
place = {Denmark}
year = {1978}
month = {Jan}
}