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Histoenzymatic and ultrastructural changes in the hepatocytes of gamma-irradiated rabbits

Journal Article:

Abstract

Ultrastructural changes and intracellular enzyme activities in the hepatocytes were studied in rabbits irradiated with 550 rads of gamma rays at 1, 3, 6, 9, 15 and 30 days after irradiation. Swelling and marked rarefaction of the mitochondrial matrix observed on the first day were followed by gradual condensation of the matrix between the 6th and 9th day. This state was accompanied by marked reduction in the succinate dehydrogenase activity, which gradually returned to the normal by the 30th day of observation. In the hyaloplasm, the most intense changes developed between the third and sixth day and were manifested by clearing of the cytoplasm and marked fragmentation of the endoplasmic membranes, with concurrent negligible decline of the lactate dehydrogenase activity and unchanged glucose-6-phosphate activity. In the Golgi apparatus, vacuolization of the cytoplasm and fragmentation of smooth membranes were most pronounced on the 6th day and were correlated with a weakened and diffuse reaction for thiamine pyrophospatase. The alkaline phosphatase activity was irregularly distributed in the lobule. The activities of lysosomal hydrolases, i.e. acid phosphatase, ..beta..-glucuronidase and non-specific esterase, had various localizations within the lobules. The strongest deviations from the normal and of longest duration (up to 9 days) were seen  More>>
Authors:
Cieciura, L; Bartel, H; Kaczmarek, B; Harazna, J; Orkisz, S [1] 
  1. Wojskowa Akademia Medyczna, Lodz (Poland)
Publication Date:
Jan 01, 1976
Product Type:
Journal Article
Reference Number:
AIX-10-455078; EDB-79-133239
Resource Relation:
Journal Name: Folia Histochem. Cytochem. (Krakow); (Poland); Journal Volume: 14:3
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; MITOCHONDRIA; BIOLOGICAL RADIATION EFFECTS; CYTOPLASM; DEHYDROGENASES; GAMMA RADIATION; GLUCOSE; IRRADIATION; LIVER; LYSOSOMES; PHOSPHATASES; RABBITS; ULTRASTRUCTURAL CHANGES; ALDEHYDES; ANIMALS; BIOLOGICAL EFFECTS; BODY; CARBOHYDRATES; CELL CONSTITUENTS; DIGESTIVE SYSTEM; ELECTROMAGNETIC RADIATION; ENZYMES; ESTERASES; GLANDS; HEXOSES; HYDROLASES; IONIZING RADIATIONS; MAMMALS; MONOSACCHARIDES; MORPHOLOGICAL CHANGES; ORGANIC COMPOUNDS; ORGANOIDS; ORGANS; OXIDOREDUCTASES; RADIATION EFFECTS; RADIATIONS; SACCHARIDES; VERTEBRATES; 560152* - Radiation Effects on Animals- Animals
OSTI ID:
6041700
Country of Origin:
Poland
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: FHCYA
Submitting Site:
INIS
Size:
Pages: 165-180
Announcement Date:

Journal Article:

Citation Formats

Cieciura, L, Bartel, H, Kaczmarek, B, Harazna, J, and Orkisz, S. Histoenzymatic and ultrastructural changes in the hepatocytes of gamma-irradiated rabbits. Poland: N. p., 1976. Web.
Cieciura, L, Bartel, H, Kaczmarek, B, Harazna, J, & Orkisz, S. Histoenzymatic and ultrastructural changes in the hepatocytes of gamma-irradiated rabbits. Poland.
Cieciura, L, Bartel, H, Kaczmarek, B, Harazna, J, and Orkisz, S. 1976. "Histoenzymatic and ultrastructural changes in the hepatocytes of gamma-irradiated rabbits." Poland.
@misc{etde_6041700,
title = {Histoenzymatic and ultrastructural changes in the hepatocytes of gamma-irradiated rabbits}
author = {Cieciura, L, Bartel, H, Kaczmarek, B, Harazna, J, and Orkisz, S}
abstractNote = {Ultrastructural changes and intracellular enzyme activities in the hepatocytes were studied in rabbits irradiated with 550 rads of gamma rays at 1, 3, 6, 9, 15 and 30 days after irradiation. Swelling and marked rarefaction of the mitochondrial matrix observed on the first day were followed by gradual condensation of the matrix between the 6th and 9th day. This state was accompanied by marked reduction in the succinate dehydrogenase activity, which gradually returned to the normal by the 30th day of observation. In the hyaloplasm, the most intense changes developed between the third and sixth day and were manifested by clearing of the cytoplasm and marked fragmentation of the endoplasmic membranes, with concurrent negligible decline of the lactate dehydrogenase activity and unchanged glucose-6-phosphate activity. In the Golgi apparatus, vacuolization of the cytoplasm and fragmentation of smooth membranes were most pronounced on the 6th day and were correlated with a weakened and diffuse reaction for thiamine pyrophospatase. The alkaline phosphatase activity was irregularly distributed in the lobule. The activities of lysosomal hydrolases, i.e. acid phosphatase, ..beta..-glucuronidase and non-specific esterase, had various localizations within the lobules. The strongest deviations from the normal and of longest duration (up to 9 days) were seen in the Browicz-Kupffer cells. Complex studies on the same material conducted concurrently with the use of different methods showed that radiation damages structure and function in unequal degrees. Moreover, within the same organ the cellular response to ionizing radiation varies according to the character, localization and functional state of the cells. Deviations from the normal state occur between the first and ninth days, most of the structural and functional elements show signs of return to the normal about the 15th day after irradiation.}
journal = {Folia Histochem. Cytochem. (Krakow); (Poland)}
volume = {14:3}
journal type = {AC}
place = {Poland}
year = {1976}
month = {Jan}
}