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Accumulation of glycation products in. cap alpha. -H pig lens crystallin and its bearing to diabetic cataract genesis

Journal Article:

Abstract

The incorporation of /sup 11/C-glucose in native pig crystalline by in vitro incubation was found, after subsequent dialysis, to affect all 5 classes of crystallin separated by Sepharose CL-6B column chromatography. Though the radioactivity of the ..cap alpha..-H fraction was three times greater than that of any of the others, autoradiographs of SDS-PAGE gels showed /sup 11/C-glucose adducts to be present in all soluble protein subunits, without there being any evidence of preferential glycation of the ..cap alpha..-H subunits. The concentration of stable glycation products in the ..cap alpha..-H chromatographic fraction of soluble crystallins is suggested to be due the addition of glycated material to this fraction as result of glycation-induced hyperaggregation, and not because the ..cap alpha..-H subunits were especially susceptible to glycation.
Publication Date:
Jan 01, 1988
Product Type:
Journal Article
Reference Number:
AIX-20-041315; EDB-89-080716
Resource Relation:
Journal Name: Acta Ophthalmol.; (Denmark); Journal Volume: 66
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DIABETES MELLITUS; CRYSTALLINE LENS; GLUCOSE; AUTORADIOGRAPHY; CARBON 14 COMPOUNDS; SWINE; ALDEHYDES; ANIMALS; BODY; BODY AREAS; CARBOHYDRATES; DISEASES; DOMESTIC ANIMALS; ENDOCRINE DISEASES; EYES; FACE; HEAD; HEXOSES; LABELLED COMPOUNDS; MAMMALS; METABOLIC DISEASES; MONOSACCHARIDES; ORGANIC COMPOUNDS; ORGANS; SACCHARIDES; SENSE ORGANS; VERTEBRATES; 560162* - Radionuclide Effects, Kinetics, & Toxicology- Animals, Plants, Microorganisms, & Cells
OSTI ID:
6034752
Research Organizations:
Chair of Physiopathology. Service of Endocrinology and Nutrition and Instituto Universitario Iberoamericano de Endocrinologia, Metabolismo y Nutricion, Hospital General de Galicia, Clinico Universitario. University of Santiago de Compostela, Santiago de Compostela (Spain)
Country of Origin:
Denmark
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: ACOPA
Submitting Site:
DKN
Size:
Pages: 66
Announcement Date:

Journal Article:

Citation Formats

Vidal, P, and Cabezas-Cerrato, J. Accumulation of glycation products in. cap alpha. -H pig lens crystallin and its bearing to diabetic cataract genesis. Denmark: N. p., 1988. Web.
Vidal, P, & Cabezas-Cerrato, J. Accumulation of glycation products in. cap alpha. -H pig lens crystallin and its bearing to diabetic cataract genesis. Denmark.
Vidal, P, and Cabezas-Cerrato, J. 1988. "Accumulation of glycation products in. cap alpha. -H pig lens crystallin and its bearing to diabetic cataract genesis." Denmark.
@misc{etde_6034752,
title = {Accumulation of glycation products in. cap alpha. -H pig lens crystallin and its bearing to diabetic cataract genesis}
author = {Vidal, P, and Cabezas-Cerrato, J}
abstractNote = {The incorporation of /sup 11/C-glucose in native pig crystalline by in vitro incubation was found, after subsequent dialysis, to affect all 5 classes of crystallin separated by Sepharose CL-6B column chromatography. Though the radioactivity of the ..cap alpha..-H fraction was three times greater than that of any of the others, autoradiographs of SDS-PAGE gels showed /sup 11/C-glucose adducts to be present in all soluble protein subunits, without there being any evidence of preferential glycation of the ..cap alpha..-H subunits. The concentration of stable glycation products in the ..cap alpha..-H chromatographic fraction of soluble crystallins is suggested to be due the addition of glycated material to this fraction as result of glycation-induced hyperaggregation, and not because the ..cap alpha..-H subunits were especially susceptible to glycation.}
journal = {Acta Ophthalmol.; (Denmark)}
volume = {66}
journal type = {AC}
place = {Denmark}
year = {1988}
month = {Jan}
}