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Biochemistry of an olfactory purinergic system: dephosphorylation of excitatory nucleotides and uptake of adenosine

Abstract

The olfactory organ of the spiny lobster, Panulirus argus, is composed of chemosensory sensilla containing the dendrites of primary chemosensory neurons. Receptors on these dendrites are activated by the nucleotides AMP, ADP, and ATP but not by the nucleoside adenosine. It is shown here that the lobster chemosensory sensilla contain enzymes that dephosphorylate excitatory nucleotides and an uptake system that internalizes the nonexcitatory dephosphorylated product adenosine. The uptake of (/sup 3/H)-adenosine is saturable with increasing concentration, linear with time for up to 3 h, sodium dependent, insensitive to moderate pH changes and has a Km of 7.1 microM and a Vmax of 5.2 fmol/sensillum/min (573 fmol/micrograms of protein/min). Double-label experiments show that sensilla dephosphorylate nucleotides extracellularly; /sup 3/H from adenine-labeled AMP or ATP is internalized, whereas 32P from phosphate-labeled nucleotides is not. The dephosphorylation of AMP is very rapid; /sup 3/H from AMP is internalized at the same rate as /sup 3/H from adenosine. Sensillar 5'-ectonucleotidase activity is inhibited by ADP and the ADP analog alpha, beta-methylene ADP. Collectively, these results indicate that the enzymes and the uptake system whereby chemosensory sensilla of the lobster inactivate excitatory nucleotides and clear adenosine from extracellular spaces are very similar to those present  More>>
Publication Date:
Oct 01, 1987
Product Type:
Journal Article
Reference Number:
NLM-87-10467; EDB-87-172201
Resource Relation:
Journal Name: J. Neurochem.; (United Kingdom); Journal Volume: 49:4
Subject:
59 BASIC BIOLOGICAL SCIENCES; ADENOSINE; UPTAKE; ADP; PHOSPHORYLATION; AMP; ATP; OLFACTORY BULBS; BIOCHEMISTRY; BIOCHEMICAL REACTION KINETICS; CHEMORECEPTORS; LOBSTERS; PHOSPHORUS 32; PURINES; SENSE ORGANS; SODIUM; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ALKALI METALS; ANIMALS; AQUATIC ORGANISMS; AROMATICS; ARTHROPODS; AZAARENES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; BRAIN; CENTRAL NERVOUS SYSTEM; CHEMICAL REACTIONS; CHEMISTRY; CRUSTACEANS; DAYS LIVING RADIOISOTOPES; DECAPODS; ELEMENTS; HETEROCYCLIC COMPOUNDS; INVERTEBRATES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LABELLED COMPOUNDS; LIGHT NUCLEI; METALS; NERVOUS SYSTEM; NUCLEI; NUCLEOSIDES; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; RADIOISOTOPES; REACTION KINETICS; RIBOSIDES; 550201* - Biochemistry- Tracer Techniques
OSTI ID:
6030959
Research Organizations:
Univ. of Florida, St. Augustine
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JONRA
Submitting Site:
NLM
Size:
Pages: 1174-1182
Announcement Date:

Citation Formats

Trapido-Rosenthal, H G, Carr, W E, and Gleeson, R A. Biochemistry of an olfactory purinergic system: dephosphorylation of excitatory nucleotides and uptake of adenosine. United Kingdom: N. p., 1987. Web. doi:10.1111/j.1471-4159.1987.tb10008.x.
Trapido-Rosenthal, H G, Carr, W E, & Gleeson, R A. Biochemistry of an olfactory purinergic system: dephosphorylation of excitatory nucleotides and uptake of adenosine. United Kingdom. doi:10.1111/j.1471-4159.1987.tb10008.x.
Trapido-Rosenthal, H G, Carr, W E, and Gleeson, R A. 1987. "Biochemistry of an olfactory purinergic system: dephosphorylation of excitatory nucleotides and uptake of adenosine." United Kingdom. doi:10.1111/j.1471-4159.1987.tb10008.x. https://www.osti.gov/servlets/purl/10.1111/j.1471-4159.1987.tb10008.x.
@misc{etde_6030959,
title = {Biochemistry of an olfactory purinergic system: dephosphorylation of excitatory nucleotides and uptake of adenosine}
author = {Trapido-Rosenthal, H G, Carr, W E, and Gleeson, R A}
abstractNote = {The olfactory organ of the spiny lobster, Panulirus argus, is composed of chemosensory sensilla containing the dendrites of primary chemosensory neurons. Receptors on these dendrites are activated by the nucleotides AMP, ADP, and ATP but not by the nucleoside adenosine. It is shown here that the lobster chemosensory sensilla contain enzymes that dephosphorylate excitatory nucleotides and an uptake system that internalizes the nonexcitatory dephosphorylated product adenosine. The uptake of (/sup 3/H)-adenosine is saturable with increasing concentration, linear with time for up to 3 h, sodium dependent, insensitive to moderate pH changes and has a Km of 7.1 microM and a Vmax of 5.2 fmol/sensillum/min (573 fmol/micrograms of protein/min). Double-label experiments show that sensilla dephosphorylate nucleotides extracellularly; /sup 3/H from adenine-labeled AMP or ATP is internalized, whereas 32P from phosphate-labeled nucleotides is not. The dephosphorylation of AMP is very rapid; /sup 3/H from AMP is internalized at the same rate as /sup 3/H from adenosine. Sensillar 5'-ectonucleotidase activity is inhibited by ADP and the ADP analog alpha, beta-methylene ADP. Collectively, these results indicate that the enzymes and the uptake system whereby chemosensory sensilla of the lobster inactivate excitatory nucleotides and clear adenosine from extracellular spaces are very similar to those present in the internal tissues of vertebrates, where nucleotides have many neuroactive effects.}
doi = {10.1111/j.1471-4159.1987.tb10008.x}
journal = {J. Neurochem.; (United Kingdom)}
volume = {49:4}
journal type = {AC}
place = {United Kingdom}
year = {1987}
month = {Oct}
}