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Fate of exogenously supplied bacterial DNA in soybean

Journal Article:

Abstract

The fate of exogenously supplied radiolabelled DNA from agrobacterium tumefaciens and micrococcus lysodeikticus was investigated in soybean tissues growing under various physiological conditions. The following observations are made: (a) Rapid degradation and reutilization of the donor DNA was observed in callus tissue culture. (b) Germinating seeds and five-day old seedlings were shown to degrade DNA in the incubation medium and to ultilize these degradation products for their own DNA synthesis. Reutilization could be almost totally suppressed the addition of unlabelled thymidine as a competitor. This allowed a detection of significant amounts of residuel donor closely but transiently associated with the plant tissues. (c) In soybean shoots dipped into a solution of donor DNA, partly this DNA was found to first migrate to the leaves where mostly labelled endogenous DNA was later found. Very large amounts of polymerized exogenous DNA were found in the regenerated roots after 12 days of culture.
Authors:
Ndiku, Luyindula [1] 
  1. Commissariat des Sciences Nucleaires, Kinshasa (Zaire). Centre Regional d'Etudes Nucleaires
Publication Date:
Jan 01, 1980
Product Type:
Journal Article
Reference Number:
AIX-12-638205; EDB-82-032457
Resource Relation:
Journal Name: Rev. Zair. Sci. Nucl.; (Zaire); Journal Volume: 1:1
Subject:
59 BASIC BIOLOGICAL SCIENCES; DNA; METABOLISM; GLYCINE HISPIDA; BACTERIA; MICROCOCCUS LYSODEICTICUS; TRACER TECHNIQUES; ISOTOPE APPLICATIONS; LEGUMINOSAE; MICROCOCCUS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PLANTS; 550501* - Metabolism- Tracer Techniques
OSTI ID:
6029148
Country of Origin:
ZR
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: RZSND
Submitting Site:
INIS
Size:
Pages: 95-104
Announcement Date:

Journal Article:

Citation Formats

Ndiku, Luyindula. Fate of exogenously supplied bacterial DNA in soybean. ZR: N. p., 1980. Web.
Ndiku, Luyindula. Fate of exogenously supplied bacterial DNA in soybean. ZR.
Ndiku, Luyindula. 1980. "Fate of exogenously supplied bacterial DNA in soybean." ZR.
@misc{etde_6029148,
title = {Fate of exogenously supplied bacterial DNA in soybean}
author = {Ndiku, Luyindula}
abstractNote = {The fate of exogenously supplied radiolabelled DNA from agrobacterium tumefaciens and micrococcus lysodeikticus was investigated in soybean tissues growing under various physiological conditions. The following observations are made: (a) Rapid degradation and reutilization of the donor DNA was observed in callus tissue culture. (b) Germinating seeds and five-day old seedlings were shown to degrade DNA in the incubation medium and to ultilize these degradation products for their own DNA synthesis. Reutilization could be almost totally suppressed the addition of unlabelled thymidine as a competitor. This allowed a detection of significant amounts of residuel donor closely but transiently associated with the plant tissues. (c) In soybean shoots dipped into a solution of donor DNA, partly this DNA was found to first migrate to the leaves where mostly labelled endogenous DNA was later found. Very large amounts of polymerized exogenous DNA were found in the regenerated roots after 12 days of culture.}
journal = {Rev. Zair. Sci. Nucl.; (Zaire)}
volume = {1:1}
journal type = {AC}
place = {ZR}
year = {1980}
month = {Jan}
}