Abstract
Oligonucleotide probes were used to identify base substitutions in 1089 revertants of hisG46 in Salmonella typhimurium that arose spontaneously or following irradiation with UV- or ..gamma..-rays. The hisG46 allele, carrying a mutant CCC codon (Pro) in place of the wild-type codon CTC (Leu69) reverted via 6 distinguishable mutational events: C to T transitions at codon sites 1 or 2, C to A or C to G transversions at codon site 1, C to A at codon site 2, and an extragenic suppressor mutation. The distribution of hisG46 revertants differed among treatments and was influenced by the DNA-repair capacity of the bacteria. Plasmid pKM101 enhanced the frequencies of both spontaneous adn induced mutations; transversion events were enhanced more efficiently by pKM101 than were transition events. Compared to Uvr/sup +/ bacteria, Uvr/sup -/ bacteria had higher frequencies of spontaneous and induced mutations; transition mutations were enhanced more efficiently than were transversion mutations. The inflence of DNA-repair activiteis on the mutational spectra provides some insights on the origins of spontaneous and UV-induced mutations. (author). 75 refs.; 4 figs.; 4 tabs.
Citation Formats
Eisenstadt, E, Kahng, L -S, Miller, J K, and Barnes, W M.
Influence of uvrB and pKM101 on the spectrum of spontaneous, UV- and gamma-ray-induced base substitutions that revert hisG46 in Salmonella typhimurium.
Netherlands: N. p.,
1989.
Web.
doi:10.1016/0027-5107(89)90050-X.
Eisenstadt, E, Kahng, L -S, Miller, J K, & Barnes, W M.
Influence of uvrB and pKM101 on the spectrum of spontaneous, UV- and gamma-ray-induced base substitutions that revert hisG46 in Salmonella typhimurium.
Netherlands.
https://doi.org/10.1016/0027-5107(89)90050-X
Eisenstadt, E, Kahng, L -S, Miller, J K, and Barnes, W M.
1989.
"Influence of uvrB and pKM101 on the spectrum of spontaneous, UV- and gamma-ray-induced base substitutions that revert hisG46 in Salmonella typhimurium."
Netherlands.
https://doi.org/10.1016/0027-5107(89)90050-X.
@misc{etde_6023931,
title = {Influence of uvrB and pKM101 on the spectrum of spontaneous, UV- and gamma-ray-induced base substitutions that revert hisG46 in Salmonella typhimurium}
author = {Eisenstadt, E, Kahng, L -S, Miller, J K, and Barnes, W M}
abstractNote = {Oligonucleotide probes were used to identify base substitutions in 1089 revertants of hisG46 in Salmonella typhimurium that arose spontaneously or following irradiation with UV- or ..gamma..-rays. The hisG46 allele, carrying a mutant CCC codon (Pro) in place of the wild-type codon CTC (Leu69) reverted via 6 distinguishable mutational events: C to T transitions at codon sites 1 or 2, C to A or C to G transversions at codon site 1, C to A at codon site 2, and an extragenic suppressor mutation. The distribution of hisG46 revertants differed among treatments and was influenced by the DNA-repair capacity of the bacteria. Plasmid pKM101 enhanced the frequencies of both spontaneous adn induced mutations; transversion events were enhanced more efficiently by pKM101 than were transition events. Compared to Uvr/sup +/ bacteria, Uvr/sup -/ bacteria had higher frequencies of spontaneous and induced mutations; transition mutations were enhanced more efficiently than were transversion mutations. The inflence of DNA-repair activiteis on the mutational spectra provides some insights on the origins of spontaneous and UV-induced mutations. (author). 75 refs.; 4 figs.; 4 tabs.}
doi = {10.1016/0027-5107(89)90050-X}
journal = []
volume = {210:1}
journal type = {AC}
place = {Netherlands}
year = {1989}
month = {Jan}
}
title = {Influence of uvrB and pKM101 on the spectrum of spontaneous, UV- and gamma-ray-induced base substitutions that revert hisG46 in Salmonella typhimurium}
author = {Eisenstadt, E, Kahng, L -S, Miller, J K, and Barnes, W M}
abstractNote = {Oligonucleotide probes were used to identify base substitutions in 1089 revertants of hisG46 in Salmonella typhimurium that arose spontaneously or following irradiation with UV- or ..gamma..-rays. The hisG46 allele, carrying a mutant CCC codon (Pro) in place of the wild-type codon CTC (Leu69) reverted via 6 distinguishable mutational events: C to T transitions at codon sites 1 or 2, C to A or C to G transversions at codon site 1, C to A at codon site 2, and an extragenic suppressor mutation. The distribution of hisG46 revertants differed among treatments and was influenced by the DNA-repair capacity of the bacteria. Plasmid pKM101 enhanced the frequencies of both spontaneous adn induced mutations; transversion events were enhanced more efficiently by pKM101 than were transition events. Compared to Uvr/sup +/ bacteria, Uvr/sup -/ bacteria had higher frequencies of spontaneous and induced mutations; transition mutations were enhanced more efficiently than were transversion mutations. The inflence of DNA-repair activiteis on the mutational spectra provides some insights on the origins of spontaneous and UV-induced mutations. (author). 75 refs.; 4 figs.; 4 tabs.}
doi = {10.1016/0027-5107(89)90050-X}
journal = []
volume = {210:1}
journal type = {AC}
place = {Netherlands}
year = {1989}
month = {Jan}
}