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Activation of human B lymphocytes. 8. Differential radiosensitivity of subpopulations of lymphoid cells involved in the polyclonally-induced PFC responses of peripheral blood B lymphocytes

Journal Article:

Abstract

The differential effect of various doses of irradiation on subpopulations of human peripheral blood lymphoid cells involved in the pokeweed mitogen induced PFC response against sheep red blood cells was studied. The plaque forming B cells were quite sensitive to low doses of irradiation with complete suppression of responses at 300 to 500 rad. On the contrary, helper T-cell function was resistant to 2000 rad. Co-culture of irradiated T cells with autologous or allogeneic B cells resulted in marked enhancement of PFC responses consistent with the suppression of naturally occurring suppressor cells with a resulting pure helper effect. Irradiated T-cell-depleted suspensions failed to produce this effect as did heat killed T cells, whereas mitomycin C treated T cells gave effects similar to irradiated T cells. These findings are consistent with a lack of requirement of cell division for a T-cell helper effect and a requirement of mitosis or another irradiation sensitive, mitomycin C sensitive process for a T-suppressor cell effect. These studies have potential relevance in the evaluation of subpopulations of human lymphoid cells involved in antibody production in normal individuals and in disease states.
Authors:
Fauci, A S; Pratt, K R; Whalen, G [1] 
  1. National Inst. of Allergy and Infectious Diseases, Bethesda, MD (USA)
Publication Date:
Nov 01, 1978
Product Type:
Journal Article
Reference Number:
AIX-10-444905; EDB-79-120346
Resource Relation:
Journal Name: Immunology; (United Kingdom); Journal Volume: 35:5
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ERYTHROCYTES; RADIOSENSITIVITY; CLONE CELLS; DOSE-RESPONSE RELATIONSHIPS; IMMUNE REACTIONS; IMMUNOGLOBULINS; IRRADIATION; LYMPHOCYTES; MAN; MITOMYCIN; MITOSIS; PLAQUE FORMATION; RADIATIONS; SHEEP; ANIMAL CELLS; ANIMALS; ANTIBIOTICS; ANTIMITOTIC DRUGS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CELL CULTURES; CELL DIVISION; CONNECTIVE TISSUE CELLS; DOMESTIC ANIMALS; DRUGS; GLOBULINS; LEUKOCYTES; MAMMALS; ORGANIC COMPOUNDS; PRIMATES; PROTEINS; RUMINANTS; SOMATIC CELLS; VERTEBRATES; 560114* - Radiation Effects on Biochemicals- In Animals- (-1987)
OSTI ID:
6000565
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: IMMUA
Submitting Site:
INIS
Size:
Pages: 715-720
Announcement Date:
May 01, 1979

Journal Article:

Citation Formats

Fauci, A S, Pratt, K R, and Whalen, G. Activation of human B lymphocytes. 8. Differential radiosensitivity of subpopulations of lymphoid cells involved in the polyclonally-induced PFC responses of peripheral blood B lymphocytes. United Kingdom: N. p., 1978. Web.
Fauci, A S, Pratt, K R, & Whalen, G. Activation of human B lymphocytes. 8. Differential radiosensitivity of subpopulations of lymphoid cells involved in the polyclonally-induced PFC responses of peripheral blood B lymphocytes. United Kingdom.
Fauci, A S, Pratt, K R, and Whalen, G. 1978. "Activation of human B lymphocytes. 8. Differential radiosensitivity of subpopulations of lymphoid cells involved in the polyclonally-induced PFC responses of peripheral blood B lymphocytes." United Kingdom.
@misc{etde_6000565,
title = {Activation of human B lymphocytes. 8. Differential radiosensitivity of subpopulations of lymphoid cells involved in the polyclonally-induced PFC responses of peripheral blood B lymphocytes}
author = {Fauci, A S, Pratt, K R, and Whalen, G}
abstractNote = {The differential effect of various doses of irradiation on subpopulations of human peripheral blood lymphoid cells involved in the pokeweed mitogen induced PFC response against sheep red blood cells was studied. The plaque forming B cells were quite sensitive to low doses of irradiation with complete suppression of responses at 300 to 500 rad. On the contrary, helper T-cell function was resistant to 2000 rad. Co-culture of irradiated T cells with autologous or allogeneic B cells resulted in marked enhancement of PFC responses consistent with the suppression of naturally occurring suppressor cells with a resulting pure helper effect. Irradiated T-cell-depleted suspensions failed to produce this effect as did heat killed T cells, whereas mitomycin C treated T cells gave effects similar to irradiated T cells. These findings are consistent with a lack of requirement of cell division for a T-cell helper effect and a requirement of mitosis or another irradiation sensitive, mitomycin C sensitive process for a T-suppressor cell effect. These studies have potential relevance in the evaluation of subpopulations of human lymphoid cells involved in antibody production in normal individuals and in disease states.}
journal = {Immunology; (United Kingdom)}
volume = {35:5}
journal type = {AC}
place = {United Kingdom}
year = {1978}
month = {Nov}
}