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Active oxygen participation in chlorophyll destruction and lipid peroxidation in SO/sub 2/-fumigated leaves of spinach

Journal Article:

Abstract

Chlorophyll a and carotenoids of spinach plants began to be destroyed in 2 to 3 hr after the initiation of fumigation with 2.0 ppM sulfur dioxide (SO/sub 2/) in light, whereas chlorophyll b was apparently undamaged during 8 hr of exposure to SO/sub 2/. The content of pheophytin a, chromatographically determined, was not changed by SO/sub 2/ fumigation. When leaf disks (phi = 10 mm), excised from the leaves fumigated with SO/sub 2/ at 2.0 ppM for 2 hr, were illuminated, chlorophyll a and carotenoids were broken down, but they were not destroyed in darkness. The destruction of chlorophyll a and carotenoids was suppressed under a stream of nitrogen. Chlorophyll a destruction was inhibited by free radical scavengers, 1,2-dihydroxbenzene-3,5-disulfonate (tiron), hydroquinone and ascorbate. The singlet oxygen scavengers, 1,4-diazabicyclo-(2,2,2)-octane (DABCO), methionine and histidine, and hydroxyl radical scavengers, benzoate and formate were without effect on the destruction of chlorophyll a. Chlorophyll a destruction was inhibited by the addition of superoxide dismutase (SOD) to the homogenate of SO/sub 2/-fumigated leaves. SO/sub 2/ fumigation for 2 hr reduced the activity of superoxide dismutase to 40% without producing the significant loss of chlorophyll. From these results we concluded that chlorophyll a destruction by SO/sub 2/  More>>
Publication Date:
Jan 01, 1980
Product Type:
Journal Article
Reference Number:
EDB-85-056292
Resource Relation:
Journal Name: Kokuritsu Kogai Kenkyusho Kenkyu Hokoku; (Japan); Journal Volume: 11
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CAROTENOIDS; DECOMPOSITION; CHLOROPHYLL; LIPIDS; OXIDATION; SULFUR DIOXIDE; BIOLOGICAL EFFECTS; AIR POLLUTION; BIOCHEMISTRY; COMPARATIVE EVALUATIONS; DAILY VARIATIONS; EXPERIMENTAL DATA; SPINACH; SUPEROXIDE RADICALS; CARBOXYLIC ACIDS; CHALCOGENIDES; CHEMICAL REACTIONS; CHEMISTRY; DATA; FOOD; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HYDROCARBONS; INFORMATION; NUMERICAL DATA; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; OXIDES; OXYGEN COMPOUNDS; PHYTOCHROMES; PIGMENTS; PLANTS; POLLUTION; PORPHYRINS; RADICALS; SULFUR COMPOUNDS; SULFUR OXIDES; TERPENES; VARIATIONS; VEGETABLES; 560303* - Chemicals Metabolism & Toxicology- Plants- (-1987)
OSTI ID:
5996210
Research Organizations:
National Inst. for Environmental Studies, Yatabe, Japan
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: KKOKD
Submitting Site:
HEDB
Size:
Pages: 91-101
Announcement Date:
Feb 01, 1985

Journal Article:

Citation Formats

Shimazaki, K, Sakaki, T, and Sugahara, K. Active oxygen participation in chlorophyll destruction and lipid peroxidation in SO/sub 2/-fumigated leaves of spinach. Japan: N. p., 1980. Web.
Shimazaki, K, Sakaki, T, & Sugahara, K. Active oxygen participation in chlorophyll destruction and lipid peroxidation in SO/sub 2/-fumigated leaves of spinach. Japan.
Shimazaki, K, Sakaki, T, and Sugahara, K. 1980. "Active oxygen participation in chlorophyll destruction and lipid peroxidation in SO/sub 2/-fumigated leaves of spinach." Japan.
@misc{etde_5996210,
title = {Active oxygen participation in chlorophyll destruction and lipid peroxidation in SO/sub 2/-fumigated leaves of spinach}
author = {Shimazaki, K, Sakaki, T, and Sugahara, K}
abstractNote = {Chlorophyll a and carotenoids of spinach plants began to be destroyed in 2 to 3 hr after the initiation of fumigation with 2.0 ppM sulfur dioxide (SO/sub 2/) in light, whereas chlorophyll b was apparently undamaged during 8 hr of exposure to SO/sub 2/. The content of pheophytin a, chromatographically determined, was not changed by SO/sub 2/ fumigation. When leaf disks (phi = 10 mm), excised from the leaves fumigated with SO/sub 2/ at 2.0 ppM for 2 hr, were illuminated, chlorophyll a and carotenoids were broken down, but they were not destroyed in darkness. The destruction of chlorophyll a and carotenoids was suppressed under a stream of nitrogen. Chlorophyll a destruction was inhibited by free radical scavengers, 1,2-dihydroxbenzene-3,5-disulfonate (tiron), hydroquinone and ascorbate. The singlet oxygen scavengers, 1,4-diazabicyclo-(2,2,2)-octane (DABCO), methionine and histidine, and hydroxyl radical scavengers, benzoate and formate were without effect on the destruction of chlorophyll a. Chlorophyll a destruction was inhibited by the addition of superoxide dismutase (SOD) to the homogenate of SO/sub 2/-fumigated leaves. SO/sub 2/ fumigation for 2 hr reduced the activity of superoxide dismutase to 40% without producing the significant loss of chlorophyll. From these results we concluded that chlorophyll a destruction by SO/sub 2/ was due to superoxide radicals. Moreover, malondialdehyde (MDA), an indicator of lipid peroxidation, was accumulated in SO/sub 2/-fumigated leaves in light. MDA formation was inhibited by tiron and hydroquinone, and by DABCO but was not inhibited by benzoate and formate. MDA formation was increased by D/sub 2/O. From these results it was concluded that /sup 1/O/sub 2/ was involved in lipid peroxidation in SO/sub 2/-fumigated leaves.}
journal = {Kokuritsu Kogai Kenkyusho Kenkyu Hokoku; (Japan)}
volume = {11}
journal type = {AC}
place = {Japan}
year = {1980}
month = {Jan}
}