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Cell biological effects of total body irradiation on growth and differentiation of acute myelogenous leukemia cells compared to normal bone marrow

Journal Article:

Abstract

Radiation therapy is used as total body treatment in preparation of the acute myelogenous leukemia (AML) patient for bone marrow transplantation. Many AML patients will have residual leukemia cells at the time of total body irradiation (TBI). In the present study, the effect of TBI on leukemic myeloid cells was compared to the effect on normal marrow granulocytic stem cells (CFUc) in vitro. Little difference from that of normal CFUc was found in the radiosensitivity of two mouse myeloid leukemia cell lines. The effect of TBI on growth of WEHI-3 or J774 cells in millipore diffusion chambers was stimulatory. These AML cell lines as well as others derived from Friend or Abelson virus infected in vitro long term mouse marrow cultures showed some morphologic differentiation by 7 days growth in diffusion chambers in irradiated heterologous rat hosts, but immature cells predominated by day 21. Thus, evidence in murine models of AML indicates that residual AML cells surviving chemotherapy will show no greater susceptibility to radiation killing compared to normal stem cells and will rapidly repopulate the irradiated host.
Publication Date:
Jan 01, 1979
Product Type:
Journal Article
Reference Number:
EDB-83-114650
Resource Relation:
Journal Name: Exp. Hematol. (Copenhagen); (Denmark); Journal Volume: 7 Suppl 5
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BONE MARROW CELLS; BIOLOGICAL RADIATION EFFECTS; MYELOID LEUKEMIA; RADIOTHERAPY; PATIENTS; WHOLE-BODY IRRADIATION; BONE MARROW; CELL CULTURES; CHEMOTHERAPY; LEUKOCYTES; RADIOSENSITIVITY EFFECTS; TRANSPLANTS; ANIMAL CELLS; ANIMAL TISSUES; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DISEASES; EXTERNAL IRRADIATION; HEMATOPOIETIC SYSTEM; HEMIC DISEASES; IRRADIATION; LEUKEMIA; MATERIALS; MEDICINE; NEOPLASMS; NUCLEAR MEDICINE; ORGANS; RADIATION EFFECTS; RADIOLOGY; SOMATIC CELLS; THERAPY; TISSUES; 550603* - Medicine- External Radiation in Therapy- (1980-); 560121 - Radiation Effects on Cells- External Source- (-1987)
OSTI ID:
5994664
Research Organizations:
Joint Center for Radiation Therapy, Department of Radiation Therapy, Harvard Medical School, Boston, MA
Country of Origin:
Denmark
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: EXHMA
Submitting Site:
HEDB
Size:
Pages: 279-289
Announcement Date:

Journal Article:

Citation Formats

Greenberger, J S, Weichselbaum, R R, Botnick, L E, Sakakeeny, M, and Moloney, W C. Cell biological effects of total body irradiation on growth and differentiation of acute myelogenous leukemia cells compared to normal bone marrow. Denmark: N. p., 1979. Web.
Greenberger, J S, Weichselbaum, R R, Botnick, L E, Sakakeeny, M, & Moloney, W C. Cell biological effects of total body irradiation on growth and differentiation of acute myelogenous leukemia cells compared to normal bone marrow. Denmark.
Greenberger, J S, Weichselbaum, R R, Botnick, L E, Sakakeeny, M, and Moloney, W C. 1979. "Cell biological effects of total body irradiation on growth and differentiation of acute myelogenous leukemia cells compared to normal bone marrow." Denmark.
@misc{etde_5994664,
title = {Cell biological effects of total body irradiation on growth and differentiation of acute myelogenous leukemia cells compared to normal bone marrow}
author = {Greenberger, J S, Weichselbaum, R R, Botnick, L E, Sakakeeny, M, and Moloney, W C}
abstractNote = {Radiation therapy is used as total body treatment in preparation of the acute myelogenous leukemia (AML) patient for bone marrow transplantation. Many AML patients will have residual leukemia cells at the time of total body irradiation (TBI). In the present study, the effect of TBI on leukemic myeloid cells was compared to the effect on normal marrow granulocytic stem cells (CFUc) in vitro. Little difference from that of normal CFUc was found in the radiosensitivity of two mouse myeloid leukemia cell lines. The effect of TBI on growth of WEHI-3 or J774 cells in millipore diffusion chambers was stimulatory. These AML cell lines as well as others derived from Friend or Abelson virus infected in vitro long term mouse marrow cultures showed some morphologic differentiation by 7 days growth in diffusion chambers in irradiated heterologous rat hosts, but immature cells predominated by day 21. Thus, evidence in murine models of AML indicates that residual AML cells surviving chemotherapy will show no greater susceptibility to radiation killing compared to normal stem cells and will rapidly repopulate the irradiated host.}
journal = {Exp. Hematol. (Copenhagen); (Denmark)}
volume = {7 Suppl 5}
journal type = {AC}
place = {Denmark}
year = {1979}
month = {Jan}
}