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Chromosome aberration assays in barley (Hordeum vulgare)

Journal Article:

Abstract

Barley is an exceellent organism for studies of induced chromosome aberrations because of its few (2n = 2x = 14) relatively large chromosomes. Root-tip and shoot-tip cells have been used extensively for the study of ionizing radiation-induced chromosome aberrations. The general procedures are well known, the technology is simple and easy to learn, and the assays are relatively quick and inexpensive. Both root tips and shoot tips can be used for the study of chemical mutagens as well as ionizing radiations. Pollen mother cells are well suited for studying the effects of mutagens on meiotic chromosomes. The literature review for the Gene-Tox Program reported on 61 chemicals tested for their effects on barley chromosomes. Of these, 90% were reported to be either positive or positive dose-related, while 7% were negative and 3% were questionable. Barley assays based on chromosomal aberrations are useful to detect the clastogenic potency of chemicals under laboratory conditions. Indications are that the data from barley can be used to corroborate data obtained from other organisms. Among the classes of chemicals assayed were: alcohols and phenols; alkaloids; epoxides; alkyl sulfates; amides and sulfonamides; aromatic amines; aryl halides; aziridines; alkenes; carbamates; hydroazides; nitroaromatics; nitrosamides; nitrosources; phenothiazines; and polycyclic  More>>
Authors:
Constantin, M J; [1]  Nilan, R A
  1. Univ. of Tennessee, Knoxville
Publication Date:
Jan 01, 1982
Product Type:
Journal Article
Reference Number:
ERA-08-044809; EDB-83-143164
Resource Relation:
Journal Name: Mutat. Res.; (Netherlands); Journal Volume: 99
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ALCOHOLS; MUTAGEN SCREENING; AMIDES; AROMATICS; BARLEY; SENSITIVITY; EPOXIDES; HALOGENATED AROMATIC HYDROCARBONS; NITROSO COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PHENOLS; POLYCYCLIC AROMATIC HYDROCARBONS; BIOASSAY; CHROMOSOMAL ABERRATIONS; COMPARATIVE EVALUATIONS; GENETIC EFFECTS; MUTAGENS; ROOTS; TOXICITY; BIOLOGICAL EFFECTS; CEREALS; GRAMINEAE; GRASS; HYDROCARBONS; HYDROXY COMPOUNDS; MUTATIONS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; PLANTS; SCREENING; 560303* - Chemicals Metabolism & Toxicology- Plants- (-1987)
OSTI ID:
5986208
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: MUREA
Submitting Site:
HEDB
Size:
Pages: 13-36
Announcement Date:

Journal Article:

Citation Formats

Constantin, M J, and Nilan, R A. Chromosome aberration assays in barley (Hordeum vulgare). Netherlands: N. p., 1982. Web.
Constantin, M J, & Nilan, R A. Chromosome aberration assays in barley (Hordeum vulgare). Netherlands.
Constantin, M J, and Nilan, R A. 1982. "Chromosome aberration assays in barley (Hordeum vulgare)." Netherlands.
@misc{etde_5986208,
title = {Chromosome aberration assays in barley (Hordeum vulgare)}
author = {Constantin, M J, and Nilan, R A}
abstractNote = {Barley is an exceellent organism for studies of induced chromosome aberrations because of its few (2n = 2x = 14) relatively large chromosomes. Root-tip and shoot-tip cells have been used extensively for the study of ionizing radiation-induced chromosome aberrations. The general procedures are well known, the technology is simple and easy to learn, and the assays are relatively quick and inexpensive. Both root tips and shoot tips can be used for the study of chemical mutagens as well as ionizing radiations. Pollen mother cells are well suited for studying the effects of mutagens on meiotic chromosomes. The literature review for the Gene-Tox Program reported on 61 chemicals tested for their effects on barley chromosomes. Of these, 90% were reported to be either positive or positive dose-related, while 7% were negative and 3% were questionable. Barley assays based on chromosomal aberrations are useful to detect the clastogenic potency of chemicals under laboratory conditions. Indications are that the data from barley can be used to corroborate data obtained from other organisms. Among the classes of chemicals assayed were: alcohols and phenols; alkaloids; epoxides; alkyl sulfates; amides and sulfonamides; aromatic amines; aryl halides; aziridines; alkenes; carbamates; hydroazides; nitroaromatics; nitrosamides; nitrosources; phenothiazines; and polycyclic aromatic hydrocarbons.}
journal = {Mutat. Res.; (Netherlands)}
volume = {99}
journal type = {AC}
place = {Netherlands}
year = {1982}
month = {Jan}
}