Abstract
The extent of strand breakage and repair in irradiated E. coli B/r and Bsub(s-l) was studied using a DNA-unwinding technique in denaturing conditions of weak alkali. Although these two strains showed widely different response to the lethal effects of ionizing radiation, they both had an equal capacity to repair radiation-induced breaks in DNA. Oxygen enhancement ratios for the killing of B/r and Bsub(s-l) were respectively 4 and 2; but after repair in non-nutrient or nutrient post-irradiation conditions, the oxygen enhancement values for the residual strand breaks were always the same for the two strains. The equal abilities of E.coli B/r and E.coli Bsub(s-l) to remove the strand breaks measured by this weak-alkali technqiue has led to the suggestion that some other type of damage to either DNA or another macromolecule may play a major role in determining whether or not the cells survive to proliferate.
Citation Formats
Ahnstroem, G, George, A M, and Cramp, W A.
Extensive and equivalent repair in both radiation-resistant and radiation-sensitive E. coli determined by a DNA-unwinding technique.
United Kingdom: N. p.,
1978.
Web.
doi:10.1080/09553007814550951.
Ahnstroem, G, George, A M, & Cramp, W A.
Extensive and equivalent repair in both radiation-resistant and radiation-sensitive E. coli determined by a DNA-unwinding technique.
United Kingdom.
https://doi.org/10.1080/09553007814550951
Ahnstroem, G, George, A M, and Cramp, W A.
1978.
"Extensive and equivalent repair in both radiation-resistant and radiation-sensitive E. coli determined by a DNA-unwinding technique."
United Kingdom.
https://doi.org/10.1080/09553007814550951.
@misc{etde_5974707,
title = {Extensive and equivalent repair in both radiation-resistant and radiation-sensitive E. coli determined by a DNA-unwinding technique}
author = {Ahnstroem, G, George, A M, and Cramp, W A}
abstractNote = {The extent of strand breakage and repair in irradiated E. coli B/r and Bsub(s-l) was studied using a DNA-unwinding technique in denaturing conditions of weak alkali. Although these two strains showed widely different response to the lethal effects of ionizing radiation, they both had an equal capacity to repair radiation-induced breaks in DNA. Oxygen enhancement ratios for the killing of B/r and Bsub(s-l) were respectively 4 and 2; but after repair in non-nutrient or nutrient post-irradiation conditions, the oxygen enhancement values for the residual strand breaks were always the same for the two strains. The equal abilities of E.coli B/r and E.coli Bsub(s-l) to remove the strand breaks measured by this weak-alkali technqiue has led to the suggestion that some other type of damage to either DNA or another macromolecule may play a major role in determining whether or not the cells survive to proliferate.}
doi = {10.1080/09553007814550951}
journal = []
volume = {34:4}
journal type = {AC}
place = {United Kingdom}
year = {1978}
month = {Oct}
}
title = {Extensive and equivalent repair in both radiation-resistant and radiation-sensitive E. coli determined by a DNA-unwinding technique}
author = {Ahnstroem, G, George, A M, and Cramp, W A}
abstractNote = {The extent of strand breakage and repair in irradiated E. coli B/r and Bsub(s-l) was studied using a DNA-unwinding technique in denaturing conditions of weak alkali. Although these two strains showed widely different response to the lethal effects of ionizing radiation, they both had an equal capacity to repair radiation-induced breaks in DNA. Oxygen enhancement ratios for the killing of B/r and Bsub(s-l) were respectively 4 and 2; but after repair in non-nutrient or nutrient post-irradiation conditions, the oxygen enhancement values for the residual strand breaks were always the same for the two strains. The equal abilities of E.coli B/r and E.coli Bsub(s-l) to remove the strand breaks measured by this weak-alkali technqiue has led to the suggestion that some other type of damage to either DNA or another macromolecule may play a major role in determining whether or not the cells survive to proliferate.}
doi = {10.1080/09553007814550951}
journal = []
volume = {34:4}
journal type = {AC}
place = {United Kingdom}
year = {1978}
month = {Oct}
}