Abstract
Various investigators have cultured murine bone marrow or peritoneal cells in vitro on glass or plastic surfaces with the ultimate aim of retrieving adherent macrophages for morphologic and functional evaluation. The removal of these adherent macrophages by conventional techniques has been consistently accompanied by low yield and significant cell damage. The authors report here a simple technique for culturing murine bone marrow cells in gelatin sponges (Spongostan and Gelfoam) in growth medium containing 10% fetal bovine serum and 10% L-cell conditioned medium. Viable cells were retrieved from the sponges in 10 min by digestion with collagenase. The in situ growth kinetics were similar to those found for cells cultured on plastic dishes. The recovered cells were adherent, phagocytic, positive for Fc ..gamma.. receptors, and had esterase activity. 23 references, 1 figure, 1 table.
Citation Formats
Akporiaye, E T, Stewart, S, and Stewart, C C.
Cultivation of murine bone marrow macrophages in sponges: a method that permits recovery of viable cultured cells.
Netherlands: N. p.,
1984.
Web.
Akporiaye, E T, Stewart, S, & Stewart, C C.
Cultivation of murine bone marrow macrophages in sponges: a method that permits recovery of viable cultured cells.
Netherlands.
Akporiaye, E T, Stewart, S, and Stewart, C C.
1984.
"Cultivation of murine bone marrow macrophages in sponges: a method that permits recovery of viable cultured cells."
Netherlands.
@misc{etde_5965785,
title = {Cultivation of murine bone marrow macrophages in sponges: a method that permits recovery of viable cultured cells}
author = {Akporiaye, E T, Stewart, S, and Stewart, C C}
abstractNote = {Various investigators have cultured murine bone marrow or peritoneal cells in vitro on glass or plastic surfaces with the ultimate aim of retrieving adherent macrophages for morphologic and functional evaluation. The removal of these adherent macrophages by conventional techniques has been consistently accompanied by low yield and significant cell damage. The authors report here a simple technique for culturing murine bone marrow cells in gelatin sponges (Spongostan and Gelfoam) in growth medium containing 10% fetal bovine serum and 10% L-cell conditioned medium. Viable cells were retrieved from the sponges in 10 min by digestion with collagenase. The in situ growth kinetics were similar to those found for cells cultured on plastic dishes. The recovered cells were adherent, phagocytic, positive for Fc ..gamma.. receptors, and had esterase activity. 23 references, 1 figure, 1 table.}
journal = []
volume = {75}
journal type = {AC}
place = {Netherlands}
year = {1984}
month = {Jan}
}
title = {Cultivation of murine bone marrow macrophages in sponges: a method that permits recovery of viable cultured cells}
author = {Akporiaye, E T, Stewart, S, and Stewart, C C}
abstractNote = {Various investigators have cultured murine bone marrow or peritoneal cells in vitro on glass or plastic surfaces with the ultimate aim of retrieving adherent macrophages for morphologic and functional evaluation. The removal of these adherent macrophages by conventional techniques has been consistently accompanied by low yield and significant cell damage. The authors report here a simple technique for culturing murine bone marrow cells in gelatin sponges (Spongostan and Gelfoam) in growth medium containing 10% fetal bovine serum and 10% L-cell conditioned medium. Viable cells were retrieved from the sponges in 10 min by digestion with collagenase. The in situ growth kinetics were similar to those found for cells cultured on plastic dishes. The recovered cells were adherent, phagocytic, positive for Fc ..gamma.. receptors, and had esterase activity. 23 references, 1 figure, 1 table.}
journal = []
volume = {75}
journal type = {AC}
place = {Netherlands}
year = {1984}
month = {Jan}
}