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Behavior of isolated nuclei and chromosomes of Rhynchosciara in vitro

Journal Article:

Abstract

Nuclei and chromosomes were isolated from salivary glands of Rhynchosciara by a method involving micropipette manipulation, after diluting the intercellular cement with a mixture of citric acid, tween 80 and sucrose. Culture media supplemented and not supplemented with different concentrations of lobster hemolymph or calf serum, were compared. In the best medium, isolated nuclei and chromosomes treated with tritiated precursors showed nucleic acid and protein synthesis.
Authors:
Cestari, A N; Simoes, L C.G. [1] 
  1. Sao Paulo Univ. (Brazil). Inst. de Biociencias
Publication Date:
Sep 01, 1980
Product Type:
Journal Article
Reference Number:
AIX-12-621961; EDB-82-004768
Resource Relation:
Journal Name: Rev. Bras. Genet.; (Brazil); Journal Volume: 3:3
Subject:
59 BASIC BIOLOGICAL SCIENCES; DNA; BIOSYNTHESIS; NUCLEOPROTEINS; RNA; AUTORADIOGRAPHY; CELL NUCLEI; CULTURE MEDIA; FLIES; IN VITRO; PRECURSOR; SALIVARY GLANDS; TRITIUM; ANIMALS; ARTHROPODS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CELL CONSTITUENTS; DIPTERA; GLANDS; HYDROGEN ISOTOPES; INSECTS; INVERTEBRATES; ISOTOPES; LIGHT NUCLEI; NUCLEI; NUCLEIC ACIDS; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOISOTOPES; SYNTHESIS; YEARS LIVING RADIOISOTOPES; 550301* - Cytology- Tracer Techniques
OSTI ID:
5952801
Country of Origin:
Brazil
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: RBGED
Submitting Site:
INIS
Size:
Pages: 303-315
Announcement Date:
Aug 01, 1981

Journal Article:

Citation Formats

Cestari, A N, and Simoes, L C.G. Behavior of isolated nuclei and chromosomes of Rhynchosciara in vitro. Brazil: N. p., 1980. Web.
Cestari, A N, & Simoes, L C.G. Behavior of isolated nuclei and chromosomes of Rhynchosciara in vitro. Brazil.
Cestari, A N, and Simoes, L C.G. 1980. "Behavior of isolated nuclei and chromosomes of Rhynchosciara in vitro." Brazil.
@misc{etde_5952801,
title = {Behavior of isolated nuclei and chromosomes of Rhynchosciara in vitro}
author = {Cestari, A N, and Simoes, L C.G.}
abstractNote = {Nuclei and chromosomes were isolated from salivary glands of Rhynchosciara by a method involving micropipette manipulation, after diluting the intercellular cement with a mixture of citric acid, tween 80 and sucrose. Culture media supplemented and not supplemented with different concentrations of lobster hemolymph or calf serum, were compared. In the best medium, isolated nuclei and chromosomes treated with tritiated precursors showed nucleic acid and protein synthesis.}
journal = {Rev. Bras. Genet.; (Brazil)}
volume = {3:3}
journal type = {AC}
place = {Brazil}
year = {1980}
month = {Sep}
}