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Cerenkov light and the production of photoreactivatable damage in X-irradiated E. coli

Journal Article:

Abstract

Survival curve data for oxygenated E. coli AB2480 irradiated with 6 MVp photons in the absence and presence of DNA are presented for bacteria which have or have not received photoreactivation treatment following x-ray exposure. At the concentration of DNA used (OD = 4.4 at 260 nm) partial protection against induction of photoreactivatable damage was attained. Following photoreactivation the survival curves had the same slope, irrespective of the presence or absence of DNA. Survival data for oxygenated E.coli AB2480 irradiated with 50 Gy of 6 MVp photons in the presence of DNA at varying concentrations (OD range 0.5 to 12) and then processed with or without exposure to photoreactivating light are also presented. Survival increased with DNA concentration in the absence, but not in the presence, of photoreactivation. It is concluded that theoretical considerations and experimental data are consistent with Cerenkov light being responsible for the production of a major part of the photoreactivatable damage induced in E. coli DNA by high energy X-, ..gamma..- or electron irradiation, but that the data obtained with low energy X-rays (300 kVp) and with high energy X-rays (6 MVp) plus DNA as a scavenger of Cerenkov light, are indicative of a component of  More>>
Authors:
Redpath, J L; Zabilansky, E; Morgan, T; [1]  Ward, J F [2] 
  1. California Univ., Irvine (USA). Dept. of Radiological Sciences
  2. California Univ., San Diego, La Jolla (USA). Dept. of Radiology
Publication Date:
May 01, 1981
Product Type:
Journal Article
Reference Number:
AIX-12-618510; EDB-82-005159
Resource Relation:
Journal Name: Int. J. Radiat. Biol. Relat. Stud. Phys., Chem. Med.; (United Kingdom); Journal Volume: 39:5
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CHERENKOV RADIATION; BIOLOGICAL RADIATION EFFECTS; ESCHERICHIA COLI; PHOTOREACTIVATION; DILUTION; DNA; ELECTRONS; GAMMA RADIATION; KEV RANGE 100-1000; MEV RANGE 01-10; OPACITY; RESPONSE MODIFYING FACTORS; SURVIVAL CURVES; ULTRAVIOLET RADIATION; VISIBLE RADIATION; X RADIATION; BACTERIA; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; ELECTROMAGNETIC RADIATION; ELEMENTARY PARTICLES; ENERGY RANGE; FERMIONS; IONIZING RADIATIONS; KEV RANGE; LEPTONS; MEV RANGE; MICROORGANISMS; NUCLEIC ACIDS; OPTICAL PROPERTIES; ORGANIC COMPOUNDS; PHYSICAL PROPERTIES; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
OSTI ID:
5950337
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: IJRBA
Submitting Site:
INIS
Size:
Pages: 569-575
Announcement Date:

Journal Article:

Citation Formats

Redpath, J L, Zabilansky, E, Morgan, T, and Ward, J F. Cerenkov light and the production of photoreactivatable damage in X-irradiated E. coli. United Kingdom: N. p., 1981. Web.
Redpath, J L, Zabilansky, E, Morgan, T, & Ward, J F. Cerenkov light and the production of photoreactivatable damage in X-irradiated E. coli. United Kingdom.
Redpath, J L, Zabilansky, E, Morgan, T, and Ward, J F. 1981. "Cerenkov light and the production of photoreactivatable damage in X-irradiated E. coli." United Kingdom.
@misc{etde_5950337,
title = {Cerenkov light and the production of photoreactivatable damage in X-irradiated E. coli}
author = {Redpath, J L, Zabilansky, E, Morgan, T, and Ward, J F}
abstractNote = {Survival curve data for oxygenated E. coli AB2480 irradiated with 6 MVp photons in the absence and presence of DNA are presented for bacteria which have or have not received photoreactivation treatment following x-ray exposure. At the concentration of DNA used (OD = 4.4 at 260 nm) partial protection against induction of photoreactivatable damage was attained. Following photoreactivation the survival curves had the same slope, irrespective of the presence or absence of DNA. Survival data for oxygenated E.coli AB2480 irradiated with 50 Gy of 6 MVp photons in the presence of DNA at varying concentrations (OD range 0.5 to 12) and then processed with or without exposure to photoreactivating light are also presented. Survival increased with DNA concentration in the absence, but not in the presence, of photoreactivation. It is concluded that theoretical considerations and experimental data are consistent with Cerenkov light being responsible for the production of a major part of the photoreactivatable damage induced in E. coli DNA by high energy X-, ..gamma..- or electron irradiation, but that the data obtained with low energy X-rays (300 kVp) and with high energy X-rays (6 MVp) plus DNA as a scavenger of Cerenkov light, are indicative of a component of the photoreactivatable damage being induced by a mechanism not involving Cerenkov light.}
journal = {Int. J. Radiat. Biol. Relat. Stud. Phys., Chem. Med.; (United Kingdom)}
volume = {39:5}
journal type = {AC}
place = {United Kingdom}
year = {1981}
month = {May}
}