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Fructose production by Zymomonas mobilis in fed-batch culture with minimal sorbitol formation

Abstract

Fed-batch cultures of Zymomonas mobilis (UQM 2864), a mutant unable to metabolise fructose, grown on diluted sugar cane syrup (200 g/l sucrose) achieved yields of 90.5 g/l fructose and 48.3 g/l ethanol with minimal sorbitol formation and complete utilization of the substrate. The effect of inoculum size on sorbitol formation in the batch stage of fed-batch fermentation are reported. Fermentation of sucrose (350 g/l) supplemented with nutrients yielded 142 g/l fructose and 76.5 g/l ethanol. Some fructose product loss at high fructose concentrations was observed. The fed-batch fermentation process offers a method for obtaining high concentrations of fructose and ethanol from sucrose materials. (orig.).
Publication Date:
Aug 01, 1989
Product Type:
Journal Article
Reference Number:
DE-89-009279; EDB-89-113977
Resource Relation:
Journal Name: Appl. Microbiol. Biotechnol.; (Germany, Federal Republic of); Journal Volume: 31:2
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; ETHANOL; PRODUCTION; FRUCTOSE; ZYMOMONAS MOBILIS; BATCH CULTURE; FERMENTATION; GLUCOSE; PILOT PLANTS; QUANTITATIVE CHEMICAL ANALYSIS; SACCHAROSE; SORBITOL; SUGAR CANE; YIELDS; ALCOHOLS; ALDEHYDES; BACTERIA; BIOCONVERSION; CARBOHYDRATES; CHEMICAL ANALYSIS; DISACCHARIDES; DIURETICS; DRUGS; FUNCTIONAL MODELS; GRASS; HEXOSES; HYDROXY COMPOUNDS; KETONES; LILIOPSIDA; MAGNOLIOPHYTA; MICROORGANISMS; MONOSACCHARIDES; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; PLANTS; REEDS; SACCHARIDES; 140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 090222 - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 550200 - Biochemistry
OSTI ID:
5945275
Research Organizations:
Queensland Univ., St. Lucia (Australia). Dept. of Microbiology; Queensland Univ., St. Lucia (Australia). Dept. of Chemical Engineering; Queensland Science and Technology Ltd., Brisbane (Australia)
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: AMBID
Submitting Site:
DE
Size:
Pages: 129-133
Announcement Date:
Dec 01, 1989

Citation Formats

Edye, L A, Johns, M R, and Ewings, K N. Fructose production by Zymomonas mobilis in fed-batch culture with minimal sorbitol formation. Germany: N. p., 1989. Web.
Edye, L A, Johns, M R, & Ewings, K N. Fructose production by Zymomonas mobilis in fed-batch culture with minimal sorbitol formation. Germany.
Edye, L A, Johns, M R, and Ewings, K N. 1989. "Fructose production by Zymomonas mobilis in fed-batch culture with minimal sorbitol formation." Germany.
@misc{etde_5945275,
title = {Fructose production by Zymomonas mobilis in fed-batch culture with minimal sorbitol formation}
author = {Edye, L A, Johns, M R, and Ewings, K N}
abstractNote = {Fed-batch cultures of Zymomonas mobilis (UQM 2864), a mutant unable to metabolise fructose, grown on diluted sugar cane syrup (200 g/l sucrose) achieved yields of 90.5 g/l fructose and 48.3 g/l ethanol with minimal sorbitol formation and complete utilization of the substrate. The effect of inoculum size on sorbitol formation in the batch stage of fed-batch fermentation are reported. Fermentation of sucrose (350 g/l) supplemented with nutrients yielded 142 g/l fructose and 76.5 g/l ethanol. Some fructose product loss at high fructose concentrations was observed. The fed-batch fermentation process offers a method for obtaining high concentrations of fructose and ethanol from sucrose materials. (orig.).}
journal = []
volume = {31:2}
journal type = {AC}
place = {Germany}
year = {1989}
month = {Aug}
}