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Radioimmunoassay and characterization of woodchuck hepatitis virus core antigen and antibody

Journal Article:

Abstract

Solid-phase radioimmunoassays for woodchuck hepatitis virus core antigen (WHcAg) and antibody (anti-WHc) were developed. WHcAg in woodchuck liver homogenates was characterized by ultracentrifugation in CsCl gradients; both heavy and light cores were obtained from the liver of an animal during acute WHV infection, which is consistent with observations in hepatitis B virus infection in man. Endpoint titers of anti-WHc were higher in chronic WHV carriers than in animals recovered from acute infections. Both IgM and IgG anti-WHc antibodies were produced by infected woodchucks. A survey of colony woodchucks demonstrated that 88/89 animals having one or more markers of past or ongoing WHV infection were positive for anti-WHc. Thus, serum anti-WHc appears to be a sensitive marker of WHV infection.
Publication Date:
Jun 01, 1985
Product Type:
Journal Article
Reference Number:
AIX-17-016899; EDB-86-066503
Resource Relation:
Journal Name: Virus Res.; (Netherlands); Journal Volume: 2:4
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; HEPATITIS; RADIOIMMUNOASSAY; ANTIBODIES; ANTIGENS; RODENTS; VIRAL DISEASES; ANIMALS; DIGESTIVE SYSTEM DISEASES; DISEASES; IMMUNOASSAY; IMMUNOLOGY; INFECTIOUS DISEASES; ISOTOPE APPLICATIONS; MAMMALS; RADIOASSAY; RADIOIMMUNOLOGY; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5941468
Research Organizations:
Georgetown Univ., Rockville, MD, USA. Schools of Medicine and Dentistry; Meloy Laboratories, Inc., Rockville, MD, USA; National Inst. of Allergy and Infectious Diseases, Bethesda, MD, USA
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: VIRED
Submitting Site:
INIS
Size:
Pages: 301-315
Announcement Date:
Feb 01, 1986

Journal Article:

Citation Formats

Ponzetto, A, Cote, P J, Ford, E C, Engle, R, Gerin, J L, Cicmanec, J, Shapiro, M, and Purcell, R H. Radioimmunoassay and characterization of woodchuck hepatitis virus core antigen and antibody. Netherlands: N. p., 1985. Web.
Ponzetto, A, Cote, P J, Ford, E C, Engle, R, Gerin, J L, Cicmanec, J, Shapiro, M, & Purcell, R H. Radioimmunoassay and characterization of woodchuck hepatitis virus core antigen and antibody. Netherlands.
Ponzetto, A, Cote, P J, Ford, E C, Engle, R, Gerin, J L, Cicmanec, J, Shapiro, M, and Purcell, R H. 1985. "Radioimmunoassay and characterization of woodchuck hepatitis virus core antigen and antibody." Netherlands.
@misc{etde_5941468,
title = {Radioimmunoassay and characterization of woodchuck hepatitis virus core antigen and antibody}
author = {Ponzetto, A, Cote, P J, Ford, E C, Engle, R, Gerin, J L, Cicmanec, J, Shapiro, M, and Purcell, R H}
abstractNote = {Solid-phase radioimmunoassays for woodchuck hepatitis virus core antigen (WHcAg) and antibody (anti-WHc) were developed. WHcAg in woodchuck liver homogenates was characterized by ultracentrifugation in CsCl gradients; both heavy and light cores were obtained from the liver of an animal during acute WHV infection, which is consistent with observations in hepatitis B virus infection in man. Endpoint titers of anti-WHc were higher in chronic WHV carriers than in animals recovered from acute infections. Both IgM and IgG anti-WHc antibodies were produced by infected woodchucks. A survey of colony woodchucks demonstrated that 88/89 animals having one or more markers of past or ongoing WHV infection were positive for anti-WHc. Thus, serum anti-WHc appears to be a sensitive marker of WHV infection.}
journal = {Virus Res.; (Netherlands)}
volume = {2:4}
journal type = {AC}
place = {Netherlands}
year = {1985}
month = {Jun}
}