Abstract
The determination of serum pepsinogen A (=pepsinogen I) levels is of clinical importance in the study of duodenal ulcer, atrophic gastritis and gastric cancer. In the present study two different quantitative immunological techniques for serum pepsinogen A were compared: a radioimmunoassay (RIA) (Helsinki) and an enzyme-linked immunosorbent assay (ELISA) (Amsterdam). Serum samples of 177 subjects with various gastric diseases were tested in a double blind study. The correlation was excellent (r=0.954 in the range 0-760 ..mu..g/l and r=0.971 in the range 0-100 ..mu..g/l). The functional relationship between ELISA (x) and RIA (y), determined by weighted model II regression, was y=1.12x-0.54. Initially the use of goat anti-PGA in the ELISA resulted in falsely high values in about 10% of the individuals. This was caused by circulating antibodies cross-reacting with goat IgG. This artefact was eliminated by pre-incubation of all samples with non-immune goat serum.
Citation Formats
Pals, G, Meuwissen, S G.M., Frants, R R, Kostense, P J, Eriksson, A W, and Raesaenen, V.
Enzyme-linked immunosorbent assay and radioimmunoassay of serum pepsinogen A.
United Kingdom: N. p.,
1987.
Web.
Pals, G, Meuwissen, S G.M., Frants, R R, Kostense, P J, Eriksson, A W, & Raesaenen, V.
Enzyme-linked immunosorbent assay and radioimmunoassay of serum pepsinogen A.
United Kingdom.
Pals, G, Meuwissen, S G.M., Frants, R R, Kostense, P J, Eriksson, A W, and Raesaenen, V.
1987.
"Enzyme-linked immunosorbent assay and radioimmunoassay of serum pepsinogen A."
United Kingdom.
@misc{etde_5931998,
title = {Enzyme-linked immunosorbent assay and radioimmunoassay of serum pepsinogen A}
author = {Pals, G, Meuwissen, S G.M., Frants, R R, Kostense, P J, Eriksson, A W, and Raesaenen, V}
abstractNote = {The determination of serum pepsinogen A (=pepsinogen I) levels is of clinical importance in the study of duodenal ulcer, atrophic gastritis and gastric cancer. In the present study two different quantitative immunological techniques for serum pepsinogen A were compared: a radioimmunoassay (RIA) (Helsinki) and an enzyme-linked immunosorbent assay (ELISA) (Amsterdam). Serum samples of 177 subjects with various gastric diseases were tested in a double blind study. The correlation was excellent (r=0.954 in the range 0-760 ..mu..g/l and r=0.971 in the range 0-100 ..mu..g/l). The functional relationship between ELISA (x) and RIA (y), determined by weighted model II regression, was y=1.12x-0.54. Initially the use of goat anti-PGA in the ELISA resulted in falsely high values in about 10% of the individuals. This was caused by circulating antibodies cross-reacting with goat IgG. This artefact was eliminated by pre-incubation of all samples with non-immune goat serum.}
journal = []
volume = {47:1}
journal type = {AC}
place = {United Kingdom}
year = {1987}
month = {Feb}
}
title = {Enzyme-linked immunosorbent assay and radioimmunoassay of serum pepsinogen A}
author = {Pals, G, Meuwissen, S G.M., Frants, R R, Kostense, P J, Eriksson, A W, and Raesaenen, V}
abstractNote = {The determination of serum pepsinogen A (=pepsinogen I) levels is of clinical importance in the study of duodenal ulcer, atrophic gastritis and gastric cancer. In the present study two different quantitative immunological techniques for serum pepsinogen A were compared: a radioimmunoassay (RIA) (Helsinki) and an enzyme-linked immunosorbent assay (ELISA) (Amsterdam). Serum samples of 177 subjects with various gastric diseases were tested in a double blind study. The correlation was excellent (r=0.954 in the range 0-760 ..mu..g/l and r=0.971 in the range 0-100 ..mu..g/l). The functional relationship between ELISA (x) and RIA (y), determined by weighted model II regression, was y=1.12x-0.54. Initially the use of goat anti-PGA in the ELISA resulted in falsely high values in about 10% of the individuals. This was caused by circulating antibodies cross-reacting with goat IgG. This artefact was eliminated by pre-incubation of all samples with non-immune goat serum.}
journal = []
volume = {47:1}
journal type = {AC}
place = {United Kingdom}
year = {1987}
month = {Feb}
}