Abstract
A procedure is described for tritium labelling of human insulin in position Tyr/sup B26/ by means of trypsin catalyzed condensation of DiBoc-DOI with (N/sup /epsilon//-Boc, /sup 3/H-Tyr/sup B26/)-IOP, subsequent deprotection and purification by HPLC. The tritium labelling of the octapeptide was accomplished by dehalotritiation of the corresponding Dit/sup B26/-octapeptide which was obtained both by iodination of N/sup /epsilon//-Boc-IOP and by total synthesis. (author). 2 figs., 1 tab., 17 refs.
Citation Formats
Haensicke, A, Kaufmann, K D, Beyermann, M, Oehlke, J, Kertscher, U, Bienert, M, Niedrich, H, Mittag, E, Bespalova, Zh D, and Titov, M I.
Synthesis of (/sup 3/H-Tyr/sup B26/)-human insulin by enzymic semisynthesis.
Serbia and Montenegro: N. p.,
1988.
Web.
Haensicke, A, Kaufmann, K D, Beyermann, M, Oehlke, J, Kertscher, U, Bienert, M, Niedrich, H, Mittag, E, Bespalova, Zh D, & Titov, M I.
Synthesis of (/sup 3/H-Tyr/sup B26/)-human insulin by enzymic semisynthesis.
Serbia and Montenegro.
Haensicke, A, Kaufmann, K D, Beyermann, M, Oehlke, J, Kertscher, U, Bienert, M, Niedrich, H, Mittag, E, Bespalova, Zh D, and Titov, M I.
1988.
"Synthesis of (/sup 3/H-Tyr/sup B26/)-human insulin by enzymic semisynthesis."
Serbia and Montenegro.
@misc{etde_5926046,
title = {Synthesis of (/sup 3/H-Tyr/sup B26/)-human insulin by enzymic semisynthesis}
author = {Haensicke, A, Kaufmann, K D, Beyermann, M, Oehlke, J, Kertscher, U, Bienert, M, Niedrich, H, Mittag, E, Bespalova, Zh D, and Titov, M I}
abstractNote = {A procedure is described for tritium labelling of human insulin in position Tyr/sup B26/ by means of trypsin catalyzed condensation of DiBoc-DOI with (N/sup /epsilon//-Boc, /sup 3/H-Tyr/sup B26/)-IOP, subsequent deprotection and purification by HPLC. The tritium labelling of the octapeptide was accomplished by dehalotritiation of the corresponding Dit/sup B26/-octapeptide which was obtained both by iodination of N/sup /epsilon//-Boc-IOP and by total synthesis. (author). 2 figs., 1 tab., 17 refs.}
journal = []
volume = {53:11B}
journal type = {AC}
place = {Serbia and Montenegro}
year = {1988}
month = {Nov}
}
title = {Synthesis of (/sup 3/H-Tyr/sup B26/)-human insulin by enzymic semisynthesis}
author = {Haensicke, A, Kaufmann, K D, Beyermann, M, Oehlke, J, Kertscher, U, Bienert, M, Niedrich, H, Mittag, E, Bespalova, Zh D, and Titov, M I}
abstractNote = {A procedure is described for tritium labelling of human insulin in position Tyr/sup B26/ by means of trypsin catalyzed condensation of DiBoc-DOI with (N/sup /epsilon//-Boc, /sup 3/H-Tyr/sup B26/)-IOP, subsequent deprotection and purification by HPLC. The tritium labelling of the octapeptide was accomplished by dehalotritiation of the corresponding Dit/sup B26/-octapeptide which was obtained both by iodination of N/sup /epsilon//-Boc-IOP and by total synthesis. (author). 2 figs., 1 tab., 17 refs.}
journal = []
volume = {53:11B}
journal type = {AC}
place = {Serbia and Montenegro}
year = {1988}
month = {Nov}
}