Abstract
A direct solid-phase radioimmunoassy (dRIA) was developed in order to demonstrate antibodies against Aujeszky's disease virus (ADV) in sera obtained from pigs and rabbits. In the presence of guinea-pig complement the above test is 160-fold to 1500-fold more sensitive than the neutralization test (NT) and 320-fold to 150 000-fold more sensitive than sera obtained from an ADV-infected farm, which were found to be negative in the complement assisted NT. It is possible to test a single dilution of unknown serum by dRIA by comparing same with a standard curve and to make a statement regarding its ADV-specific binding capacity to /sup 125/I-labelled ADV antigen. The advantages of dRIA in comparison to the indirect RIA and the advantages and disadvantages with regard to ELISA were discussed.
Citation Formats
Doeller, G, and Jakubik, J.
Direct solid-phase radioimmunoassay for the detection of Aujeszky's disease antibodies.
Germany: N. p.,
1980.
Web.
Doeller, G, & Jakubik, J.
Direct solid-phase radioimmunoassay for the detection of Aujeszky's disease antibodies.
Germany.
Doeller, G, and Jakubik, J.
1980.
"Direct solid-phase radioimmunoassay for the detection of Aujeszky's disease antibodies."
Germany.
@misc{etde_5925322,
title = {Direct solid-phase radioimmunoassay for the detection of Aujeszky's disease antibodies}
author = {Doeller, G, and Jakubik, J}
abstractNote = {A direct solid-phase radioimmunoassy (dRIA) was developed in order to demonstrate antibodies against Aujeszky's disease virus (ADV) in sera obtained from pigs and rabbits. In the presence of guinea-pig complement the above test is 160-fold to 1500-fold more sensitive than the neutralization test (NT) and 320-fold to 150 000-fold more sensitive than sera obtained from an ADV-infected farm, which were found to be negative in the complement assisted NT. It is possible to test a single dilution of unknown serum by dRIA by comparing same with a standard curve and to make a statement regarding its ADV-specific binding capacity to /sup 125/I-labelled ADV antigen. The advantages of dRIA in comparison to the indirect RIA and the advantages and disadvantages with regard to ELISA were discussed.}
journal = []
volume = {247:1}
journal type = {AC}
place = {Germany}
year = {1980}
month = {Jun}
}
title = {Direct solid-phase radioimmunoassay for the detection of Aujeszky's disease antibodies}
author = {Doeller, G, and Jakubik, J}
abstractNote = {A direct solid-phase radioimmunoassy (dRIA) was developed in order to demonstrate antibodies against Aujeszky's disease virus (ADV) in sera obtained from pigs and rabbits. In the presence of guinea-pig complement the above test is 160-fold to 1500-fold more sensitive than the neutralization test (NT) and 320-fold to 150 000-fold more sensitive than sera obtained from an ADV-infected farm, which were found to be negative in the complement assisted NT. It is possible to test a single dilution of unknown serum by dRIA by comparing same with a standard curve and to make a statement regarding its ADV-specific binding capacity to /sup 125/I-labelled ADV antigen. The advantages of dRIA in comparison to the indirect RIA and the advantages and disadvantages with regard to ELISA were discussed.}
journal = []
volume = {247:1}
journal type = {AC}
place = {Germany}
year = {1980}
month = {Jun}
}