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Direct solid-phase radioimmunoassay for the detection of Aujeszky's disease antibodies

Journal Article:

Abstract

A direct solid-phase radioimmunoassy (dRIA) was developed in order to demonstrate antibodies against Aujeszky's disease virus (ADV) in sera obtained from pigs and rabbits. In the presence of guinea-pig complement the above test is 160-fold to 1500-fold more sensitive than the neutralization test (NT) and 320-fold to 150 000-fold more sensitive than sera obtained from an ADV-infected farm, which were found to be negative in the complement assisted NT. It is possible to test a single dilution of unknown serum by dRIA by comparing same with a standard curve and to make a statement regarding its ADV-specific binding capacity to /sup 125/I-labelled ADV antigen. The advantages of dRIA in comparison to the indirect RIA and the advantages and disadvantages with regard to ELISA were discussed.
Publication Date:
Jun 01, 1980
Product Type:
Journal Article
Reference Number:
EDB-82-042583
Resource Relation:
Journal Name: Zentralbl. Bakteriol., Parasitenkd., Infektionskr. Hyg., Abt. 1: Orig., Reihe A; (Germany, Federal Republic of); Journal Volume: 247:1
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 60 APPLIED LIFE SCIENCES; ANTIBODIES; RADIOIMMUNOASSAY; INFECTIOUS DISEASES; DIAGNOSIS; COMPARATIVE EVALUATIONS; RABBITS; SENSITIVITY; SWINE; VIRUSES; ANIMALS; DISEASES; DOMESTIC ANIMALS; ISOTOPE APPLICATIONS; MAMMALS; MICROORGANISMS; PARASITES; RADIOASSAY; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics; 553001 - Agriculture & Food Technology- Tracer Techniques- (-1987)
OSTI ID:
5925322
Country of Origin:
Germany
Language:
German
Other Identifying Numbers:
Journal ID: CODEN: ZMMPA
Submitting Site:
DE
Size:
Pages: 1-7
Announcement Date:

Journal Article:

Citation Formats

Doeller, G, and Jakubik, J. Direct solid-phase radioimmunoassay for the detection of Aujeszky's disease antibodies. Germany: N. p., 1980. Web.
Doeller, G, & Jakubik, J. Direct solid-phase radioimmunoassay for the detection of Aujeszky's disease antibodies. Germany.
Doeller, G, and Jakubik, J. 1980. "Direct solid-phase radioimmunoassay for the detection of Aujeszky's disease antibodies." Germany.
@misc{etde_5925322,
title = {Direct solid-phase radioimmunoassay for the detection of Aujeszky's disease antibodies}
author = {Doeller, G, and Jakubik, J}
abstractNote = {A direct solid-phase radioimmunoassy (dRIA) was developed in order to demonstrate antibodies against Aujeszky's disease virus (ADV) in sera obtained from pigs and rabbits. In the presence of guinea-pig complement the above test is 160-fold to 1500-fold more sensitive than the neutralization test (NT) and 320-fold to 150 000-fold more sensitive than sera obtained from an ADV-infected farm, which were found to be negative in the complement assisted NT. It is possible to test a single dilution of unknown serum by dRIA by comparing same with a standard curve and to make a statement regarding its ADV-specific binding capacity to /sup 125/I-labelled ADV antigen. The advantages of dRIA in comparison to the indirect RIA and the advantages and disadvantages with regard to ELISA were discussed.}
journal = {Zentralbl. Bakteriol., Parasitenkd., Infektionskr. Hyg., Abt. 1: Orig., Reihe A; (Germany, Federal Republic of)}
volume = {247:1}
journal type = {AC}
place = {Germany}
year = {1980}
month = {Jun}
}