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Enhanced biological phosphorus removal. Carbon sources, nitrate as electron acceptor, and characterization of the sludge community

Abstract

Enhanced biological phosphorus removal (EBPR) was studied in laboratory scale experiments as well as in a full scale EBPR process. The studies were focused on carbon source transformations, the use of nitrate as an electron acceptor and characterisation of the microflora. A continuous anaerobic/aerobic laboratory system was operated on synthetic wastewater with acetate as sole carbon source. An efficient EBPR was obtained and mass balances over the anaerobic reactor showed a production of 1.45 g poly-{beta}-hydroxyalcanoic acids (PHA), measured as chemical oxygen demand (COD), per g of acetic acid (as COD) taken up. Furthermore, phosphate was released in the anaerobic reactor in a ratio of 0.33 g phosphorus (P) per g PHA (COD) formed and 0.64 g of glycogen (COD) was consumed per g of acetic acid (COD) taken up. Microscopic investigations revealed a high amount of polyphosphate accumulating organisms (PAO) in the sludge. Isolation and characterisation of bacteria indicated Acinetobacter spp. to be abundant in the sludge, while sequencing of clones obtained in a 16S rDNA clone library showed a large part of the bacteria to be related to the high mole % G+C Gram-positive bacteria and only a minor fraction to be related to the gamma-subclass of proteobacteria  More>>
Authors:
Publication Date:
Oct 01, 1997
Product Type:
Thesis/Dissertation
Report Number:
LUTKDH-TKBT-97-1034
Reference Number:
SCA: 540320; PA: SWD-98:007100; EDB-98:054070; NTS-98:005134; SN: 98001944091
Resource Relation:
Other Information: TH: Diss. (TeknD); PBD: Oct 1997
Subject:
54 ENVIRONMENTAL SCIENCES; REMOVAL; PHOSPHORUS; WASTE WATER; SEWAGE SLUDGE; CARBON SOURCES; BENCH-SCALE EXPERIMENTS; WATER POLLUTION CONTROL; NITRATES
OSTI ID:
591494
Research Organizations:
Lund Univ. (Sweden). Dept. of Biotechnology
Country of Origin:
Sweden
Language:
English
Other Identifying Numbers:
Other: ON: DE98750758; TRN: SE9807100
Availability:
OSTI as DE98750758
Submitting Site:
SWD
Size:
88 p.
Announcement Date:
Jun 02, 1998

Citation Formats

Christensson, M. Enhanced biological phosphorus removal. Carbon sources, nitrate as electron acceptor, and characterization of the sludge community. Sweden: N. p., 1997. Web.
Christensson, M. Enhanced biological phosphorus removal. Carbon sources, nitrate as electron acceptor, and characterization of the sludge community. Sweden.
Christensson, M. 1997. "Enhanced biological phosphorus removal. Carbon sources, nitrate as electron acceptor, and characterization of the sludge community." Sweden.
@misc{etde_591494,
title = {Enhanced biological phosphorus removal. Carbon sources, nitrate as electron acceptor, and characterization of the sludge community}
author = {Christensson, M}
abstractNote = {Enhanced biological phosphorus removal (EBPR) was studied in laboratory scale experiments as well as in a full scale EBPR process. The studies were focused on carbon source transformations, the use of nitrate as an electron acceptor and characterisation of the microflora. A continuous anaerobic/aerobic laboratory system was operated on synthetic wastewater with acetate as sole carbon source. An efficient EBPR was obtained and mass balances over the anaerobic reactor showed a production of 1.45 g poly-{beta}-hydroxyalcanoic acids (PHA), measured as chemical oxygen demand (COD), per g of acetic acid (as COD) taken up. Furthermore, phosphate was released in the anaerobic reactor in a ratio of 0.33 g phosphorus (P) per g PHA (COD) formed and 0.64 g of glycogen (COD) was consumed per g of acetic acid (COD) taken up. Microscopic investigations revealed a high amount of polyphosphate accumulating organisms (PAO) in the sludge. Isolation and characterisation of bacteria indicated Acinetobacter spp. to be abundant in the sludge, while sequencing of clones obtained in a 16S rDNA clone library showed a large part of the bacteria to be related to the high mole % G+C Gram-positive bacteria and only a minor fraction to be related to the gamma-subclass of proteobacteria to which Acinetobacter belongs. Operation of a similar anaerobic/aerobic laboratory system with ethanol as sole carbon source showed that a high EBPR can be achieved with this compound as carbon source. However, a prolonged detention time in the anaerobic reactor was required. PHA were produced in the anaerobic reactor in an amount of 1.24 g COD per g of soluble DOC taken up, phosphate was released in an amount of 0.4-0.6 g P per g PHA (COD) produced and 0.46 g glycogen (COD) was consumed per g of soluble COD taken up. Studies of the EBPR in the UCT process at the sewage treatment plant in Helsingborg, Sweden, showed the amount of volatile fatty acids (VFA) available to the PAO in the anaerobic stage to be limiting phosphorous removal. Operating the primary clarifier to obtain an enhanced hydrolysis and fermentation of primary sludge was shown to generate extra VFA and improve EBPR. VFA were shown to be transformed to PHA in the anaerobic stage of the plant, giving a PHA production of 1.5 g COD per g VFA (COD). Phosphate-P was released in an amount of 0.31 g per g PHA (COD) produced. Nitrate was used as an electron acceptor for oxidation of stored PHA in the anoxic stage of the plant during simultaneous P-uptake. Approximately 30% of the P-uptake could be accounted for by this reaction 209 refs, 25 figs, 7 tabs}
place = {Sweden}
year = {1997}
month = {Oct}
}