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Repair of ultraviolet-light damaged ColE1 factor carrying Escherichia coli genes for guanine synthesis

Journal Article:

Abstract

Hybrid ColE1 plasmids called ColE1-cos lambda-guaA or ColE1-cos lambda-gal can be efficiently transduced into various E.coli K-12 cells through packaging into lambda phage particles. Using these plasmids, repair of ultraviolet-light (UV) damaged ColE1 DNAs was studied in various UV sensitive E.coli K-12 mutants. The host mutations uvrA and uvrB markedly reduced host-cell reactivation of UV-irradiated ColE1-cos lambda-guaA. Pre-existing hybrid ColE1 plasmids had no effect on the frequency of lambda phage-mediated transduction of another differentially marked hybrid ColE1 DNAs. ColE1-cos lambda-guaA and ColE1-cos lambda-gal DNAs could temporarily but not stably co-exist in E.coli K-12 recA cells. The presence of ColE1-cos lambda-gal in uvrB cells promoted the repair of super-infected UV-irradiated ColE1-cos lambda-guaA about 7-fold. The same ColE1-cos lambda-gal plasmid in a uvrB recA double mutant did not have this promoting effect. These results indicate that the effect of resident hybrid ColE1 plasmids is manifested by the host recA/sup +/ gene function(s) and suggest that ColE1 plasmit itself provides no recA/sup +/-like functions.
Authors:
Kibe, A; Shimada, K; Tagaki, Y [1] 
  1. Kyushu Univ., Fukuoka (Japan). Dept. of Biochemistry
Publication Date:
Jan 01, 1979
Product Type:
Journal Article
Reference Number:
AIX-10-455055; EDB-79-133124
Resource Relation:
Journal Name: Mol. Gen. Genet.; (Germany, Federal Republic of); Journal Volume: 168:3
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; BIOLOGICAL REPAIR; BACTERIOPHAGES; BIOSYNTHESIS; ESCHERICHIA COLI; GENETIC RADIATION EFFECTS; GUANINE; IN VIVO; RADIATION DOSES; RADIOSENSITIVITY; SURVIVAL CURVES; ULTRAVIOLET RADIATION; AMINES; BACTERIA; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; DOSES; ELECTROMAGNETIC RADIATION; GENETIC EFFECTS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PARASITES; PURINES; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; SYNTHESIS; VIRUSES; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
OSTI ID:
5909009
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: MGGEA
Submitting Site:
INIS
Size:
Pages: 293-298
Announcement Date:

Journal Article:

Citation Formats

Kibe, A, Shimada, K, and Tagaki, Y. Repair of ultraviolet-light damaged ColE1 factor carrying Escherichia coli genes for guanine synthesis. Germany: N. p., 1979. Web.
Kibe, A, Shimada, K, & Tagaki, Y. Repair of ultraviolet-light damaged ColE1 factor carrying Escherichia coli genes for guanine synthesis. Germany.
Kibe, A, Shimada, K, and Tagaki, Y. 1979. "Repair of ultraviolet-light damaged ColE1 factor carrying Escherichia coli genes for guanine synthesis." Germany.
@misc{etde_5909009,
title = {Repair of ultraviolet-light damaged ColE1 factor carrying Escherichia coli genes for guanine synthesis}
author = {Kibe, A, Shimada, K, and Tagaki, Y}
abstractNote = {Hybrid ColE1 plasmids called ColE1-cos lambda-guaA or ColE1-cos lambda-gal can be efficiently transduced into various E.coli K-12 cells through packaging into lambda phage particles. Using these plasmids, repair of ultraviolet-light (UV) damaged ColE1 DNAs was studied in various UV sensitive E.coli K-12 mutants. The host mutations uvrA and uvrB markedly reduced host-cell reactivation of UV-irradiated ColE1-cos lambda-guaA. Pre-existing hybrid ColE1 plasmids had no effect on the frequency of lambda phage-mediated transduction of another differentially marked hybrid ColE1 DNAs. ColE1-cos lambda-guaA and ColE1-cos lambda-gal DNAs could temporarily but not stably co-exist in E.coli K-12 recA cells. The presence of ColE1-cos lambda-gal in uvrB cells promoted the repair of super-infected UV-irradiated ColE1-cos lambda-guaA about 7-fold. The same ColE1-cos lambda-gal plasmid in a uvrB recA double mutant did not have this promoting effect. These results indicate that the effect of resident hybrid ColE1 plasmids is manifested by the host recA/sup +/ gene function(s) and suggest that ColE1 plasmit itself provides no recA/sup +/-like functions.}
journal = {Mol. Gen. Genet.; (Germany, Federal Republic of)}
volume = {168:3}
journal type = {AC}
place = {Germany}
year = {1979}
month = {Jan}
}