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ATP regeneration with enzymes of the alcohol fermentation pathway and kinases of yeast and its computer simulation

Journal Article:

Abstract

Enzymes of the alcohol fermentation pathway, adenosine kinase and adenylate kinase were extracted by incubating acetone-dried yeast cells with a simple reaction medium containing glucose, and subjected to gel filtration to remove the intermediates of alcohol fermentation formed during incubation. By using the enzyme systems as catalysts and glucose as an energy source, ATP was regenerated from adenosine. A minimum concentration of fructose 1,6-bisphosphate (FBP) of 7 mM or of ATP of 10 mM was necessary to initiate the alcohol fermentation; and concentrations of nucleotides changed abruptly with reaction time. These nonlinear phenomena might be due to action of FBPase and/or ATPase as well as the complex multienzyme systems. To understand the experimentally observed phenomena, a mathematical model of the reaction system was proposed which takes into account ATP regeneration. The calculated time-dependent concentrations of glucose, FBP, adenosine and nucleotides were in agreement with experimental values qualitatively as well as quantitatively. Moreover, the nonlinear phenomena, that is, the existence of threshold concentrations of FBP and ATP below which the reaction can not proceed and the steep changes of nucleotide concentrations were also accounted for. These results indicate that the model was quite suitable for this reaction system and useful for  More>>
Publication Date:
Aug 01, 1981
Product Type:
Journal Article
Reference Number:
EDB-82-046205
Resource Relation:
Journal Name: J. Ferment. Technol.; (Japan); Journal Volume: 59:4
Subject:
09 BIOMASS FUELS; ATP; REGENERATION; FERMENTATION; BIOLOGICAL PATHWAYS; COMPUTERIZED SIMULATION; YEASTS; PHOSPHOTRANSFERASES; ALCOHOLS; FILTRATION; GLUCOSE; MATHEMATICAL MODELS; PRODUCTION; ALDEHYDES; BIOCONVERSION; CARBOHYDRATES; ENZYMES; FUNGI; HEXOSES; HYDROXY COMPOUNDS; MICROORGANISMS; MONOSACCHARIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; PLANTS; SACCHARIDES; SEPARATION PROCESSES; SIMULATION; TRANSFERASES; 090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
OSTI ID:
5901729
Research Organizations:
Kyoto Univ., Japan
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JFTED
Submitting Site:
SAI
Size:
Pages: 239-245
Announcement Date:

Journal Article:

Citation Formats

Asada, M, Shirai, Y, Nakanishi, K, Matsuno, R, Kimura, A, and Kamikubo, T. ATP regeneration with enzymes of the alcohol fermentation pathway and kinases of yeast and its computer simulation. Japan: N. p., 1981. Web.
Asada, M, Shirai, Y, Nakanishi, K, Matsuno, R, Kimura, A, & Kamikubo, T. ATP regeneration with enzymes of the alcohol fermentation pathway and kinases of yeast and its computer simulation. Japan.
Asada, M, Shirai, Y, Nakanishi, K, Matsuno, R, Kimura, A, and Kamikubo, T. 1981. "ATP regeneration with enzymes of the alcohol fermentation pathway and kinases of yeast and its computer simulation." Japan.
@misc{etde_5901729,
title = {ATP regeneration with enzymes of the alcohol fermentation pathway and kinases of yeast and its computer simulation}
author = {Asada, M, Shirai, Y, Nakanishi, K, Matsuno, R, Kimura, A, and Kamikubo, T}
abstractNote = {Enzymes of the alcohol fermentation pathway, adenosine kinase and adenylate kinase were extracted by incubating acetone-dried yeast cells with a simple reaction medium containing glucose, and subjected to gel filtration to remove the intermediates of alcohol fermentation formed during incubation. By using the enzyme systems as catalysts and glucose as an energy source, ATP was regenerated from adenosine. A minimum concentration of fructose 1,6-bisphosphate (FBP) of 7 mM or of ATP of 10 mM was necessary to initiate the alcohol fermentation; and concentrations of nucleotides changed abruptly with reaction time. These nonlinear phenomena might be due to action of FBPase and/or ATPase as well as the complex multienzyme systems. To understand the experimentally observed phenomena, a mathematical model of the reaction system was proposed which takes into account ATP regeneration. The calculated time-dependent concentrations of glucose, FBP, adenosine and nucleotides were in agreement with experimental values qualitatively as well as quantitatively. Moreover, the nonlinear phenomena, that is, the existence of threshold concentrations of FBP and ATP below which the reaction can not proceed and the steep changes of nucleotide concentrations were also accounted for. These results indicate that the model was quite suitable for this reaction system and useful for predicting the experimental results.}
journal = {J. Ferment. Technol.; (Japan)}
volume = {59:4}
journal type = {AC}
place = {Japan}
year = {1981}
month = {Aug}
}