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Molecular characterization of thymidine kinase mutants of human cells induced by densely ionizing radiation

Abstract

In order to characterize the nature of mutants induced by densely ionizing radiations at an autosomal locus, the authors have isolated a series of 99 thymidine kinase (tk) mutants of human TK6 lymphoblastoid cells iraadiated with either fast neutrons or accelerated argon ions. Individual muant clones were examined for alterations in their restriction fragment pattern after hybridization with a human cDNA probe for tk. A restriction fragment length polymorphism (RFLP) allowed identification of the active tk allele. Among the neutron-induced mutants, 34/52 exhibited loss of the previously active allele while 6/52 exhibited intragenic rearrangements. Among the argon-induced mutants 27/46 exhibited allele loses and 10/46 showed rearrangements within the tk locus. The remaining mutants had restriction patterns indistinguishable from the TK6 parent. Each of the mutant clones was further examined for structural alterations within the c-erbAl locus which has been localized to chromosome 17q11-q22, at some unknown distance from the human tk locus at chromosome 17q21-q22. A substantial proportion (54%) of tk mutants induced by densely ionizing radiation showed loss of the c-erb locus on the homologous chromosome, suggesting that the mutations involve large-scale genetic changes. (author). 51 refs.; 2 figs.; 6 tabs.
Publication Date:
Apr 01, 1989
Product Type:
Journal Article
Reference Number:
AIX-20-059872; EDB-89-118580
Resource Relation:
Journal Name: Mutat. Res., Fundam. Mol. Mech. Mutagen.; (Netherlands); Journal Volume: 211:2
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; ARGON IONS; RADIOBIOLOGY; FAST NEUTRONS; PHOSPHOTRANSFERASES; GENE MUTATIONS; DNA HYBRIDIZATION; ENZYME INDUCTION; GENE OPERONS; GENETIC RADIATION EFFECTS; HUMAN CHROMOSOME 17; LYMPHOCYTES; MAN; RADIATION INDUCED MUTANTS; RFLPS; ANIMAL CELLS; ANIMALS; BARYONS; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BIOLOGICAL RADIATION EFFECTS; BIOLOGY; BLOOD; BLOOD CELLS; BODY FLUIDS; CHARGED PARTICLES; CHROMOSOMES; CONNECTIVE TISSUE CELLS; ELEMENTARY PARTICLES; ENZYMES; FERMIONS; GENE REGULATION; GENETIC EFFECTS; HADRONS; HYBRIDIZATION; IONS; LEUKOCYTES; MAMMALS; MATERIALS; MUTANTS; MUTATIONS; NEUTRONS; NUCLEONS; PHOSPHORUS-GROUP TRANSFERASES; PRIMATES; RADIATION EFFECTS; SOMATIC CELLS; TRANSFERASES; VERTEBRATES; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture; 550200 - Biochemistry
OSTI ID:
5893767
Research Organizations:
Harvard School of Public Health, Boston, MA (USA)
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: MUREA
Submitting Site:
INIS
Size:
Pages: 215-224
Announcement Date:

Citation Formats

Kronenberg, A, and Little, J B. Molecular characterization of thymidine kinase mutants of human cells induced by densely ionizing radiation. Netherlands: N. p., 1989. Web. doi:10.1016/0027-5107(89)90004-3.
Kronenberg, A, & Little, J B. Molecular characterization of thymidine kinase mutants of human cells induced by densely ionizing radiation. Netherlands. doi:10.1016/0027-5107(89)90004-3.
Kronenberg, A, and Little, J B. 1989. "Molecular characterization of thymidine kinase mutants of human cells induced by densely ionizing radiation." Netherlands. doi:10.1016/0027-5107(89)90004-3. https://www.osti.gov/servlets/purl/10.1016/0027-5107(89)90004-3.
@misc{etde_5893767,
title = {Molecular characterization of thymidine kinase mutants of human cells induced by densely ionizing radiation}
author = {Kronenberg, A, and Little, J B}
abstractNote = {In order to characterize the nature of mutants induced by densely ionizing radiations at an autosomal locus, the authors have isolated a series of 99 thymidine kinase (tk) mutants of human TK6 lymphoblastoid cells iraadiated with either fast neutrons or accelerated argon ions. Individual muant clones were examined for alterations in their restriction fragment pattern after hybridization with a human cDNA probe for tk. A restriction fragment length polymorphism (RFLP) allowed identification of the active tk allele. Among the neutron-induced mutants, 34/52 exhibited loss of the previously active allele while 6/52 exhibited intragenic rearrangements. Among the argon-induced mutants 27/46 exhibited allele loses and 10/46 showed rearrangements within the tk locus. The remaining mutants had restriction patterns indistinguishable from the TK6 parent. Each of the mutant clones was further examined for structural alterations within the c-erbAl locus which has been localized to chromosome 17q11-q22, at some unknown distance from the human tk locus at chromosome 17q21-q22. A substantial proportion (54%) of tk mutants induced by densely ionizing radiation showed loss of the c-erb locus on the homologous chromosome, suggesting that the mutations involve large-scale genetic changes. (author). 51 refs.; 2 figs.; 6 tabs.}
doi = {10.1016/0027-5107(89)90004-3}
journal = {Mutat. Res., Fundam. Mol. Mech. Mutagen.; (Netherlands)}
volume = {211:2}
journal type = {AC}
place = {Netherlands}
year = {1989}
month = {Apr}
}