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Clinical study on insulin receptors of mononuclear cells in diabetes

Journal Article:

Abstract

/sup 125/I-insulin binding activity to mononuclear cells was studied in 75 noninsulin-dependent diabetic subjects and 31 normal subjects and the following results were obtained. 1. /sup 125/I-insulin binding is directly proportional to the mononuclear cell concentrations. There is a linear increase of specific /sup 125/I-insulin binding. 2. The binding of /sup 125/I-insulin to mononuclear cells is displaced by the increasing concentration of native insulin. 3. The /sup 125/I-insulin degradation in the incubation medium after incubation of mononuclear cells for 24 hours at 4/sup 0/C was almost 5% in this study. 4. The insulin binding activity in diabetic subjects was lower than that in normal subjects (P < 0.001) without any significant difference in affinity constant. 5. The relationship of binding activity to age of diabetics (r = 0.06, N.S), relative body weitht (r = 0.06, N.S) and duration of diabetes from onset was not significant. 6. In untreated noninsulin-dependent diabetics the insulin binding activity was inversely correlated to fasting blood glucose level (r = 0.78, P < 0.001) and slightly inversely correlated to serum insulin level (r = 0.47, P < 0.01). A slight inverse correlation was also observed in serum triglyceride level (r = 0.53, P < 0.01) and  More>>
Authors:
Dalimunthe, D [1] 
  1. Hiroshima Univ. (Japan). School of Medicine
Publication Date:
Dec 01, 1980
Product Type:
Journal Article
Reference Number:
AIX-12-634861; EDB-82-022848
Resource Relation:
Journal Name: Hiroshima J. Med. Sci.; (Japan); Journal Volume: 29:4
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; INSULIN; RADIORECEPTOR ASSAY; RECEPTORS; MONOCYTES; AFFINITY; CELL CULTURES; CHOLESTEROL; DIABETES MELLITUS; GLUCOSE; IODINE 125; LABELLED COMPOUNDS; METABOLISM; PATIENTS; RETINA; TRIGLYCERIDES; ALDEHYDES; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY AREAS; BODY FLUIDS; CARBOHYDRATES; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; ENDOCRINE DISEASES; ESTERS; EYES; FACE; HEAD; HEXOSES; HORMONES; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LEUKOCYTES; LIPIDS; MATERIALS; METABOLIC DISEASES; MONOSACCHARIDES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; RADIOISOTOPES; SACCHARIDES; SENSE ORGANS; STEROIDS; STEROLS; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5878356
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: HIJMA
Submitting Site:
INIS
Size:
Pages: 203-218
Announcement Date:

Journal Article:

Citation Formats

Dalimunthe, D. Clinical study on insulin receptors of mononuclear cells in diabetes. Japan: N. p., 1980. Web.
Dalimunthe, D. Clinical study on insulin receptors of mononuclear cells in diabetes. Japan.
Dalimunthe, D. 1980. "Clinical study on insulin receptors of mononuclear cells in diabetes." Japan.
@misc{etde_5878356,
title = {Clinical study on insulin receptors of mononuclear cells in diabetes}
author = {Dalimunthe, D}
abstractNote = {/sup 125/I-insulin binding activity to mononuclear cells was studied in 75 noninsulin-dependent diabetic subjects and 31 normal subjects and the following results were obtained. 1. /sup 125/I-insulin binding is directly proportional to the mononuclear cell concentrations. There is a linear increase of specific /sup 125/I-insulin binding. 2. The binding of /sup 125/I-insulin to mononuclear cells is displaced by the increasing concentration of native insulin. 3. The /sup 125/I-insulin degradation in the incubation medium after incubation of mononuclear cells for 24 hours at 4/sup 0/C was almost 5% in this study. 4. The insulin binding activity in diabetic subjects was lower than that in normal subjects (P < 0.001) without any significant difference in affinity constant. 5. The relationship of binding activity to age of diabetics (r = 0.06, N.S), relative body weitht (r = 0.06, N.S) and duration of diabetes from onset was not significant. 6. In untreated noninsulin-dependent diabetics the insulin binding activity was inversely correlated to fasting blood glucose level (r = 0.78, P < 0.001) and slightly inversely correlated to serum insulin level (r = 0.47, P < 0.01). A slight inverse correlation was also observed in serum triglyceride level (r = 0.53, P < 0.01) and in total cholesterol level (r = 0.29, P < 0.05). 7. No significant difference between the binding activity was observed by grade of diabetic retinopathy. 8. After treatment with diet and/or sulfonylurea, the diabetics exhibited a significant increase in insulin binding activity (P < 0.005) but no significant difference in plasma insulin level, body weight and plasma lipid levels was observed.}
journal = {Hiroshima J. Med. Sci.; (Japan)}
volume = {29:4}
journal type = {AC}
place = {Japan}
year = {1980}
month = {Dec}
}