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In vitro study on chromosomal abnormality, proliferation and differentiation of hemopoietic stem cells from atomic bomb exposures and patients with acute leukemia and related disorders

Journal Article:

Abstract

By using in vitro cloning method and cytogenetic analysis from single hemopoietic colonies, the kinetics of differentiation and proliferation, and chromosomal abnormalities of hemopoietic stem cells (CFU-GM, BFU-E) from atomic bomb exposures and patients with acute leukemia and myelodysplastic syndrome (MDS) were investigated. High incidence (24 colonies/ 206 total colonies; 11.7%) of irradiation-induced chromosomal abnormalities was observed in circulating hemopoietic stem cells from 21 high dose exposures. In two individuals, karyotypic abnormalities closely resembling those observed in the peripheral T lymphocytes were also seen in the myeloid stem cells (BFU-E). This finding suggests that totipotential stem cells common to myeloid and lymphoid lineages exist in healthy human adults and atomic bomb irradiation produced chromosomal aberrations in them. In each of 5 MDS cases with both GM colony and erythroid burst formation, identical marker chromosomes were seen in CFU-GM and BFU-E. This finding demonstrates that MDS is a clonal hemopathy that originates in a pluripotential stem cell. Whether acute leukemia is also a clonal hemopathy originating in a pluripotential stem cell remains to be determined. But there was a considerable heterogeneity in kinetics of proliferation and in responsiveness to colony stimulating factors among these cases. (author) 54 refs.
Publication Date:
Dec 01, 1988
Product Type:
Journal Article
Reference Number:
JPN-89-010345; EDB-89-127146
Resource Relation:
Journal Name: Nagasaki Igakkai Zasshi; (Japan); Journal Volume: 63:4
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; A-BOMB SURVIVORS; CHROMOSOMAL ABERRATIONS; BONE MARROW; CELL CULTURES; CELL DIFFERENTIATION; CELL PROLIFERATION; COLONY FORMATION; LEUKEMIA; LYMPHOCYTES; PATIENTS; RADIATION DOSES; ANIMAL CELLS; ANIMAL TISSUES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DISEASES; DOSES; HEMATOPOIETIC SYSTEM; HEMIC DISEASES; HUMAN POPULATIONS; IMMUNE SYSTEM DISEASES; LEUKOCYTES; MATERIALS; MUTATIONS; NEOPLASMS; ORGANS; POPULATIONS; SOMATIC CELLS; TISSUES; 560151* - Radiation Effects on Animals- Man
OSTI ID:
5874877
Research Organizations:
Nagasaki Univ. (Japan). School of Medicine
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: CODEN: NAGZA
Submitting Site:
JPN
Size:
Pages: 414-429
Announcement Date:
Apr 01, 1989

Journal Article:

Citation Formats

Amenomori, Tatsuhiko. In vitro study on chromosomal abnormality, proliferation and differentiation of hemopoietic stem cells from atomic bomb exposures and patients with acute leukemia and related disorders. Japan: N. p., 1988. Web.
Amenomori, Tatsuhiko. In vitro study on chromosomal abnormality, proliferation and differentiation of hemopoietic stem cells from atomic bomb exposures and patients with acute leukemia and related disorders. Japan.
Amenomori, Tatsuhiko. 1988. "In vitro study on chromosomal abnormality, proliferation and differentiation of hemopoietic stem cells from atomic bomb exposures and patients with acute leukemia and related disorders." Japan.
@misc{etde_5874877,
title = {In vitro study on chromosomal abnormality, proliferation and differentiation of hemopoietic stem cells from atomic bomb exposures and patients with acute leukemia and related disorders}
author = {Amenomori, Tatsuhiko}
abstractNote = {By using in vitro cloning method and cytogenetic analysis from single hemopoietic colonies, the kinetics of differentiation and proliferation, and chromosomal abnormalities of hemopoietic stem cells (CFU-GM, BFU-E) from atomic bomb exposures and patients with acute leukemia and myelodysplastic syndrome (MDS) were investigated. High incidence (24 colonies/ 206 total colonies; 11.7%) of irradiation-induced chromosomal abnormalities was observed in circulating hemopoietic stem cells from 21 high dose exposures. In two individuals, karyotypic abnormalities closely resembling those observed in the peripheral T lymphocytes were also seen in the myeloid stem cells (BFU-E). This finding suggests that totipotential stem cells common to myeloid and lymphoid lineages exist in healthy human adults and atomic bomb irradiation produced chromosomal aberrations in them. In each of 5 MDS cases with both GM colony and erythroid burst formation, identical marker chromosomes were seen in CFU-GM and BFU-E. This finding demonstrates that MDS is a clonal hemopathy that originates in a pluripotential stem cell. Whether acute leukemia is also a clonal hemopathy originating in a pluripotential stem cell remains to be determined. But there was a considerable heterogeneity in kinetics of proliferation and in responsiveness to colony stimulating factors among these cases. (author) 54 refs.}
journal = {Nagasaki Igakkai Zasshi; (Japan)}
volume = {63:4}
journal type = {AC}
place = {Japan}
year = {1988}
month = {Dec}
}