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Double-label autoradiographic deoxyglucose method for sequential measurement of regional cerebral glucose utilization

Abstract

A new double-label autoradiographic glucose analog method for the sequential measurement of altered regional cerebral metabolic rates for glucose in the same animal is presented. This method is based on the sequential injection of two boluses of glucose tracer labeled with two different isotopes (short-lived /sup 18/F and long-lived /sup 3/H, respectively). An operational equation is derived which allows the determination of glucose utilization for the time period before the injection of the second tracer; this equation corrects for accumulation and loss of the first tracer from the metabolic pool occurring after the injection of the second tracer. An error analysis of this operational equation is performed. The double-label deoxyglucose method is validated in the primary somatosensory (''barrel'') cortex of the anesthetized rat. Two different rows of whiskers were stimulated sequentially in each rat; the two periods of stimulation were each preceded by an injection of glucose tracer. After decapitation, dried brain slices were first exposed, in direct contact, to standard X-ray film and then to uncoated, ''tritium-sensitive'' film. Results show that the double-label deoxyglucose method proposed in this paper allows the quantification and complete separation of glucose utilization patterns elicited by two different stimulations sequentially applied in the same  More>>
Publication Date:
Aug 01, 1987
Product Type:
Journal Article
Reference Number:
GBN-87-007107; EDB-88-021270
Resource Relation:
Journal Name: Neuroscience; (United Kingdom); Journal Volume: 22:2
Subject:
60 APPLIED LIFE SCIENCES; GLUCOSE; AUTORADIOGRAPHY; CEREBRUM; METABOLISM; FLUORINE 18; RATS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ALDEHYDES; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; BODY; BRAIN; CARBOHYDRATES; CENTRAL NERVOUS SYSTEM; FLUORINE ISOTOPES; HEXOSES; HOURS LIVING RADIOISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; LIGHT NUCLEI; MAMMALS; MONOSACCHARIDES; NERVOUS SYSTEM; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; RADIOISOTOPES; RODENTS; SACCHARIDES; VERTEBRATES; 553006* - Agriculture & Food Technology- Other Agricultural Applications- (1987-)
OSTI ID:
5849085
Research Organizations:
Montreal Neurological Inst., Quebec, Canada; McGill Univ., Montreal, Quebec, Canada
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: NRSCD
Submitting Site:
GBN
Size:
Pages: 601-619
Announcement Date:

Citation Formats

Redies, C, Diksic, M, Evans, A C, Gjedde, A, and Yamamoto, Y L. Double-label autoradiographic deoxyglucose method for sequential measurement of regional cerebral glucose utilization. United Kingdom: N. p., 1987. Web. doi:10.1016/0306-4522(87)90357-5.
Redies, C, Diksic, M, Evans, A C, Gjedde, A, & Yamamoto, Y L. Double-label autoradiographic deoxyglucose method for sequential measurement of regional cerebral glucose utilization. United Kingdom. doi:10.1016/0306-4522(87)90357-5.
Redies, C, Diksic, M, Evans, A C, Gjedde, A, and Yamamoto, Y L. 1987. "Double-label autoradiographic deoxyglucose method for sequential measurement of regional cerebral glucose utilization." United Kingdom. doi:10.1016/0306-4522(87)90357-5. https://www.osti.gov/servlets/purl/10.1016/0306-4522(87)90357-5.
@misc{etde_5849085,
title = {Double-label autoradiographic deoxyglucose method for sequential measurement of regional cerebral glucose utilization}
author = {Redies, C, Diksic, M, Evans, A C, Gjedde, A, and Yamamoto, Y L}
abstractNote = {A new double-label autoradiographic glucose analog method for the sequential measurement of altered regional cerebral metabolic rates for glucose in the same animal is presented. This method is based on the sequential injection of two boluses of glucose tracer labeled with two different isotopes (short-lived /sup 18/F and long-lived /sup 3/H, respectively). An operational equation is derived which allows the determination of glucose utilization for the time period before the injection of the second tracer; this equation corrects for accumulation and loss of the first tracer from the metabolic pool occurring after the injection of the second tracer. An error analysis of this operational equation is performed. The double-label deoxyglucose method is validated in the primary somatosensory (''barrel'') cortex of the anesthetized rat. Two different rows of whiskers were stimulated sequentially in each rat; the two periods of stimulation were each preceded by an injection of glucose tracer. After decapitation, dried brain slices were first exposed, in direct contact, to standard X-ray film and then to uncoated, ''tritium-sensitive'' film. Results show that the double-label deoxyglucose method proposed in this paper allows the quantification and complete separation of glucose utilization patterns elicited by two different stimulations sequentially applied in the same animal.}
doi = {10.1016/0306-4522(87)90357-5}
journal = {Neuroscience; (United Kingdom)}
volume = {22:2}
journal type = {AC}
place = {United Kingdom}
year = {1987}
month = {Aug}
}